The role of the endothelium in the control of venous tone: studies on isolated human veins

Thulesius, Olav; Ugaily-Thulesius, L.; Neglén, Peter; H, Shuhaiber

doi:10.1111/j.1475-097x.1988.tb00279.x

Cited by 23 publications

(6 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Endothelium-dependent relaxation in porcine vena cordis magna elicited by substance P was rather low compared to the almost complete relaxation observed in porcine coronary artery after the same experimental procedure (Kojda et al, 1990;1992c Vanhoutte & Miller, 1985) and human coronary (Ku et al, 1992) and peripheral blood vessels (Thom et al, 1987;Thulesius et al, 1988;Lischer et al, 1988). In human coronary veins the magnitude of endothelium-dependent relaxation has been shown to be dependent on the agonist used and varied between 59 and 41% (Ku et al, 1992 oxide derived from SNAP seems to decrease the activity of GTN by an additional mechanism.…”

Section: Discussionmentioning

confidence: 98%

Nitrovasodilator‐induced relaxation and tolerance development in porcine vena cordis magna: dependence on intact endothelium

Kojda

Beck

Meyer

et al. 1994

British J Pharmacology

View full text Add to dashboard Cite

1 Isolated segments of porcine vena cordis magna exhibited a reproducible contractile activity upon application of prostaglandin F2, (PGF2.) or KCl, that was independent of the presence of intact endothelium. Substance P (3nM) elicited strictly endothelium-dependent relaxations amounting to 46.1 ± 1.4% (n = 206) of contractions induced by 10 jOM PGF2,. 2 S-nitroso-N-acetyl-D,L-penicillamine (SNAP), a compound that spontaneously liberates nitric oxide, concentration-dependently relaxed PGF2.-precontracted (501iM) venous segments. Tolerance induction (incubation with 100 LM SNAP for 30 min) within the same segments resulted in a 3 fold attenuation of this effect, which was not further reduced after additional preincubation with glyceryl trinitrate (GTN). Removal of endothelium or the presence of N0-nitro-L-arginine methylester (L-NAME) significantly improved the potency of SNAP before and after tolerance induction. 3 Concentration-dependent relaxations induced by GTN in non-tolerant veins were similar in the presence and absence of endothelium but much more reduced in tolerant endothelium-denuded (75 fold) compared to intact (20 fold) segments. In contrast, the presence of L-NAME significantly improved GTN-activity solely in non-tolerant veins, which, therefore, also resulted in a more pronounced attenuation of activity due to tolerance induction (100 fold). Preincubation of intact veins with SNAP also reduced GTN-activity but to a lesser extent (10 fold). 4 The more delayed but much longer, and compared to GTN somewhat weaker, acting new nitrovasodilator N-(3-nitrato-pivaloyl)-1-cysteineethylester (SPM 3672) was more potent in denuded than intact non-tolerant venous segments. Induction of tolerance by GTN resulted in a 2 fold-attenuation of potency. This effect was increased to 15 fold in denuded veins but solely due to enhanced potency of SPM 3672 caused by removal of endothelium. 5 These data demonstrate that intact endothelium of porcine vena cordis magna attenuates the relaxant potency of nitrovasodilators but also probably participates in vascular bioactivation of GTN. We suggest that the reduced potency of nitrovasodilators is due to endogenous production of nitric oxide, which may affect the soluble guanylate cyclase/cyclic GMP-system or inhibit nitrate bioactivation pathways.

show abstract

Section: Discussionmentioning

confidence: 98%

Nitrovasodilator‐induced relaxation and tolerance development in porcine vena cordis magna: dependence on intact endothelium

Kojda

Beck

Meyer

et al. 1994

British J Pharmacology

View full text Add to dashboard Cite

show abstract

“…One ring served as control and the other was de-endothelialized by inserting the tip of a small forceps and gently rolling the ring for 15 s on a flat cork-board. This procedure has been developed in our laboratory and is shown to remove completely the endothelium without causing any visible damage to the underlying smooth muscle coat as demonstrated in our previous ultrastructural study (Thulesius et al, 1988a).…”

Section: Methodsmentioning

confidence: 99%

Endothelial mediated enhancement of noradrenaline induced vasoconstriction in normal and varicose veins

Thulesius

Said

et al. 1991

Clinical Physiology

Self Cite

View full text Add to dashboard Cite

The role of the endothelium to influence smooth muscle contraction in normal and varicose veins was investigated in vitro using saphenous vein preparations obtained at vascular surgery. Paired control and endothelium-denuded rings were tested simultaneously and contracted with noradrenaline (10(-9)-10(-4) M). Identical experiments were also performed on sheep femoral veins and arteries. Noradrenaline induced maximal tension was 30% lower in varicose compared to normal veins. In normal veins removal of endothelium significantly reduced the maximal response to noradrenaline by 40% whereas in varicose veins no significant reduction could be seen. In the sheep femoral vein removal of the endothelium also resulted in decrease of noradrenaline-induced contraction. It can be concluded that in the human saphenous vein the endothelium has a contraction facilitating effect in response to stimulation with noradrenaline. In varicose veins the endothelial-mediated enhancement of noradrenaline-induced vasoconstriction is reduced probably because of endothelial damage. This observation may be of importance in the pathogenesis of varicose veins.

show abstract

“…of Clinical Physiology, University Hospital, S-58185 Linkoping, Sweden shown to remove endothelial cells without damaging the underlying smooth muscle layer [7]. The left anterior descending coronary artery (LAD) was identified and dissected free at the origin, and the vessel was cut into rings of 4-5 mm length.…”

Section: Blood Vesselsmentioning

confidence: 99%

Effect of the molsidomine metabolite SIN-1 on coronary arteries and peripheral vessels of sheep with special reference to tolerance and endothelium

Mariam

Yousif

Thulesius

1991

Cardiovasc Drug Ther

View full text Add to dashboard Cite

In vitro experiments on rings from coronary arteries, femoral arteries, and femoral veins of sheep were performed, and cumulative concentration-relaxation responses were established for glyceryl trinitrate (GTN) and the molsidomine metabolite SIN-1. Paired preparations of control and deendothelialized coronary artery rings were used, and the vessels were precontracted with different agonists at a concentration that elicited 30% of maximal contractions (EC-30). In coronary arteries, the responses for GTN and SIN-1 on normal and deendothelialized preparations were not significantly different. In coronary arteries preincubated with 0.44 mM GTN or SIN-1 to study tolerance development, there was a significant loss of efficacy to the relaxant effect of GTN, whereas the effect SIN-1 was essentially maintained. Femoral arteries and veins were readily relaxed with GTN. and SIN-1. In veins relaxation in relation to resting tone was much more pronounced than in coronary or femoral arteries. In conclusion, the molsidomine metabolite SIN-1 is a potent coronary and venous vasodilator that does not induce tolerance.

show abstract

The role of the endothelium in the control of venous tone: studies on isolated human veins

Cited by 23 publications

References 11 publications

Nitrovasodilator‐induced relaxation and tolerance development in porcine vena cordis magna: dependence on intact endothelium

Nitrovasodilator‐induced relaxation and tolerance development in porcine vena cordis magna: dependence on intact endothelium

Endothelial mediated enhancement of noradrenaline induced vasoconstriction in normal and varicose veins

Effect of the molsidomine metabolite SIN-1 on coronary arteries and peripheral vessels of sheep with special reference to tolerance and endothelium

Contact Info

Product

Resources

About