The Role of the Extracellular Matrix Components in Cutaneous Wound Healing

Olczyk, Paweł; Mencner, Łukasz; Komosińska-Vassev, Katarzyna

doi:10.1155/2014/747584

Cited by 305 publications

(334 citation statements)

References 87 publications

(178 reference statements)

Supporting

Mentioning

291

Contrasting

Unclassified

Order By: Relevance

“…It is a secreted serine protease that converts the proenzyme plasminogen to plasmin and plays a critical role in cell migration and tissue remodeling. Keratinocytes use plasmin activated by plasminogen activators for paving their way through phase of the wound-healing process (47), and its inhibition, as seen in our study and also reported by others (34,48), is one of the main reason for the delay in wound closure. Ctgf and transforming growth factor b1 (Tgfb1) were significantly upregulated.…”

Section: Resultssupporting

confidence: 86%

Downregulation of miRNAs during Delayed Wound Healing in Diabetes: Role of Dicer

Bhattacharya

Aggarwal

Singh

et al. 2015

Mol Med

View full text Add to dashboard Cite

Delayed wound healing is a major complication associated with diabetes and is a result of a complex interplay among diverse deregulated cellular parameters. Although several genes and pathways have been identified to be mediating impaired wound closure, the role of microRNAs (miRNAs) in these events is not very well understood. Here, we identify an altered miRNA signature in the prolonged inflammatory phase in a wound during diabetes, with increased infiltration of inflammatory cells in the basal layer of the epidermis. Nineteen miRNAs were downregulated in diabetic rat wounds (as compared with normal rat wound, d 7 postwounding) together with inhibited levels of the central miRNA biosynthesis enzyme, Dicer, suggesting that in wounds of diabetic rats, the decreased levels of Dicer are presumably responsible for miRNA downregulation. Compared with unwounded skin, Dicer levels were significantly upregulated 12 d postwounding in normal rats, and this result was notably absent in diabetic rats that showed impaired wound closure. In a wound-healing specific quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) array, 10 genes were significantly altered in the diabetic rat wound and included growth factors and collagens. Network analyses demonstrated significant interactions and correlations between the miRNA predicted targets (regulators) and the 10 wound-healing specific genes, suggesting altered miRNAs might fine-tune the levels of these genes that determine wound closure. Dicer inhibition prevented HaCaT cell migration and affected wound closure. Altered levels of Dicer and miRNAs are critical during delayed wound closure and offer promising targets to address the issue of impaired wound healing.

show abstract

Section: Resultssupporting

confidence: 86%

Downregulation of miRNAs during Delayed Wound Healing in Diabetes: Role of Dicer

Bhattacharya

Aggarwal

Singh

et al. 2015

Mol Med

View full text Add to dashboard Cite

show abstract

“…Triolein is a major form of triglyceride in human skin, which presents as subcutaneous fat and epidermal surface lipid. The apparent decrease in triolein as cancer progresses could be due to: (1) the reduction of subcutaneous fat sampled by the probe, caused by the thickening epidermis during lesion formation; and/or (2) the reduction of membrane lipid synthesis induced by UV damage [34]. Because subcutaneous fat exists in a substantial amount and has large Raman scattering cross section [41] we believe (1) is the major reason.…”

Section: Discussionmentioning

confidence: 99%

“…Collagen fibers account for about 70% of the weight of dry dermis, while elastin maintains skin elasticity through a durable cross-linked array. Large diameter elastin-rich elastic fibers reside in the reticular dermis [34]. Figure 4 illustrates Raman imaging performed on a BCC skin section to extract dermal ECM proteins.…”

Section: Dermal Ecmmentioning

confidence: 99%

Raman active components of skin cancer

Feng¹,

Moy²,

Nguyen³

et al. 2017

Biomed. Opt. Express

View full text Add to dashboard Cite

Raman spectroscopy (RS) has shown great potential in noninvasive cancer screening. Statistically based algorithms, such as principal component analysis, are commonly employed to provide tissue classification; however, they are difficult to relate to the chemical and morphological basis of the spectroscopic features and underlying disease. As a result, we propose the first Raman biophysical model applied to in vivo skin cancer screening data. We expand upon previous models by utilizing in situ skin constituents as the building blocks, and validate the model using previous clinical screening data collected from a Raman optical fiber probe. We built an 830nm confocal Raman microscope integrated with a confocal laser-scanning microscope. Raman imaging was performed on skin sections spanning various disease states, and multivariate curve resolution (MCR) analysis was used to resolve the Raman spectra of individual in situ skin constituents. The basis spectra of the most relevant skin constituents were combined linearly to fit in vivo human skin spectra. Our results suggest collagen, elastin, keratin, cell nucleus, triolein, ceramide, melanin and water are the most important model components. We make available for download (see supplemental information) a database of Raman spectra for these eight components for others to use as a reference. Our model reveals the biochemical and structural makeup of normal, nonmelanoma and melanoma skin cancers, and precancers and paves the way for future development of this approach to noninvasive skin cancer diagnosis.

show abstract

“…Interface with ECM components is regulated by mediators such as cytokines and growth factors (e.g. interleukins, interferons and TNF-α) (Olczyk et al 2014). The inflammatory response is triggered, and components such as growth factors facilitate proliferation, differentiation and metabolism of cells involved in the healing process.…”

Section: Wound Carementioning

confidence: 99%

“…The inflammatory response is triggered, and components such as growth factors facilitate proliferation, differentiation and metabolism of cells involved in the healing process. Others help regulate inflammatory processes and play a chemotactic role (encouraging cell movement) for neutrophils, macrophages, fibroblasts and epithelial cells (keratinocytes) to stimulate angiogenesis and the formation of ECM (Olczyk et al 2014).…”

Section: Wound Carementioning

confidence: 99%

Graft-Versus-Host Disease (GvHD)

Murray

Stringer

Hutt

2017

The European Blood and Marrow Transplantation Textbook for Nurses

View full text Add to dashboard Cite

Acute and chronic graft-versus-host disease (GvHD) is a major cause of morbidity and mortality in patients who undergo allogeneic haematopoietic cell transplantation (HCT) and affects approximately 30-40% of recipients. Its diagnosis is complicated, and staging of the disease varies dependent upon the transplant centre involved. Standardisation through the use of National Institute of Health (NIH) guidelines helps clinicians diagnose and treat their patients more effectively. For the majority of patients who go on to develop GvHD, corticosteroids remain the first-line treatment for both acute and chronic GvHD. Recipients that are refractory to systemic steroids have a plethora of second-and third-line options available to them. A 'standard of care' approach has not yet become agreed globally due to poor evidence from small and limited randomised control trials. Supportive care is paramount, and the nurse is often at the centre of the patients care and in the best position to guide and advise the patient and family through this often long-term complication.

show abstract

The Role of the Extracellular Matrix Components in Cutaneous Wound Healing

Cited by 305 publications

References 87 publications

Downregulation of miRNAs during Delayed Wound Healing in Diabetes: Role of Dicer

Downregulation of miRNAs during Delayed Wound Healing in Diabetes: Role of Dicer

Raman active components of skin cancer

Graft-Versus-Host Disease (GvHD)

Contact Info

Product

Resources

About