The Role of the House Dust Mite-Induced Innate Immunity in Development of Allergic Response

Jacquet, Alain

doi:10.1159/000320375

Cited by 48 publications

(45 citation statements)

References 182 publications

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“…24,25) GM-CSF released from bronchial epithelial cells of asthmatic patients can prolong the survival of eosinophils and enhance the release of mediators from those cells, contributing to the pathogenesis of airway hypersensitiveness. 26,27) HDM-stimulated airway epithelial cells also secrete large amounts of GM-CSF, RANTES and Eotaxin. These pro-inflammatory, pro-Th2 cytokines as well as chemokines recruit and activate Th2 cells, granulocytes (eosinophils, neutrophils, and basophils) and dendritic cells.…”

Section: Discussionmentioning

confidence: 99%

Effect of 1.8-Cineole in Dermatophagoides pteronyssinus-Stimulated Bronchial Epithelial Cells and Mouse Model of Asthma

Lee

Song

Park

et al. 2016

Biological & Pharmaceutical Bulletin

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1.8-Cineole (eucalyptol) is a phytoncide, a volatile organic compound derived from plants. Phytoncides are known to have an anti-inflammatory effect. However, the effects of 1.8-cineole in house dust mite (HDM)-stimulated bronchial epithelial cells are poorly understood. The objective of this study was to assess the effect of 1.8-cineole in HDM-stimulated bronchial epithelial cells and in the HDM-induced murine asthma model. The purpose of the present study is to evaluate the anti-inflammatory effects and mechanism of 1.8-cineole action in HDM-induced airway inflammation. Human bronchial epithelial cells (HBECs) were cultured with Dermatophagoides pteronyssinus (Der p) and 1.8-cineole. Cytokine protein levels, phosphorylation of protein kinases, and intracellular Toll-like receptor 4 (TLR4) expressions were measured. In the murine model, BALB/C mice were sensitized with Der p and were exposed to Der p via intranasal route during the challenge period. 1.8-Cineole was given by inhalation 6 h before the each challenge. Treatment with 1.8-cineole inhibited the Der p-induced cytokine protein expression, phosphorylation of p38 mitogen-activated protein kinase (MAPK) and Akt and intracellular TLR4 expression in HBECs. In the Der p-induced mouse model, airway hyper-responsiveness (AHR) and the number of eosinophils in bronchoalveolar lavage fluid (BALF) was also significantly reduced by 1.8-cineole treatment. The treatment of 1.8-cineole inhibited the increased production of interleukin (IL)-4, IL-13 and IL-17A in BALF after Der p challenge. These results suggest that 1.8-cineole suppresses Der p-induced IL-8, IL-6 and granulocyte macrophage-colony stimulating factor (GM-CSF) production in HBECs. Finally, we confirmed that 1.8-cineole decreases AHR and eosinophilic airway inflammation in Der p-induced asthma mice.

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Section: Discussionmentioning

confidence: 99%

Effect of 1.8-Cineole in Dermatophagoides pteronyssinus-Stimulated Bronchial Epithelial Cells and Mouse Model of Asthma

Lee

Song

Park

et al. 2016

Biological & Pharmaceutical Bulletin

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“…The theory is that persistent type 2 responses are a symptom of erroneous-instructed antigen-presenting cells combined with a Th2-biased microenvironment in both the local environment and the lymphatic tissues of the airways. Whether homeostasis in non-allergic individuals is maintained due to the triggering of mechanisms that are not present in certain individuals genetically susceptible to allergy, or if allergic individuals have an exaggerated response towards antigens, is still debated [7,8]. There are now emerging data supporting the belief that epithelial cell barrier dysfunction leads to an overzealous immune activation [9,10,11,12,13].…”

Section: Introductionmentioning

confidence: 99%

Polarized Airway Epithelial Models for Immunological Co-Culture Studies

Papazian

Würtzen

Hansen

2016

Int Arch Allergy Immunol

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Epithelial cells line all cavities and surfaces throughout the body and play a substantial role in maintaining tissue homeostasis. Asthma and other atopic diseases are increasing worldwide and allergic disorders are hypothesized to be a consequence of a combination of dysregulation of the epithelial response towards environmental antigens and genetic susceptibility, resulting in inflammation and T cell-derived immune responses. In vivo animal models have long been used to study immune homeostasis of the airways but are limited by species restriction and lack of exposure to a natural environment of both potential allergens and microflora. Limitations of these models prompt a need to develop new human cell-based in vitro models. A variety of co-culture systems for modelling the respiratory epithelium exist and are available to the scientific community. The models have become increasingly sophisticated and specific care needs to be taken with regard to cell types, culture medium and culture models, depending on the aim of the study. Although great strides have been made, there is still a need for further optimization, and optimally also for standardization, in order for in vitro co-culture models to become powerful tools in the discovery of key molecules dictating immunity and/or tolerance, and for understanding the complex interplay that takes place between mucosa, airway epithelium and resident or infiltrating immune cells. This review focuses on current knowledge and the advantages and limitations of the different cell types and culture methods used in co-culture models of the human airways.

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“…Der p 1 is a 25-kDa cysteine protease belonging to the papain-like protease family (CA1) and is a major mite allergen, as more than 80% of the HDM allergic patients develop sensitizations to this protease [2,3]. Moreover, the proteolytic activity of Der p 1 has been demonstrated to be closely related to the development of allergic diseases, notably through cleavages of tight junction proteins, immune receptors, but also the maturation of other HDM protease allergens, such as Der p 3 and Der p 6 [4,5,6,7,8,9,10,11,12]. Although the biological roles of these proteases are not well understood, they have been found in the mites’ gut and feces, suggesting their possible role in digestion [7].…”

Section: Introductionmentioning

confidence: 99%

The Lys-Asp-Tyr Triad within the Mite Allergen Der p 1 Propeptide Is a Critical Structural Element for the pH-Dependent Initiation of the Protease Maturation

Chevigné

Campizi

Szpakowska

et al. 2017

IJMS

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The major house dust mite allergen, Der p 1, is a papain-like cysteine protease expressed as an inactive precursor, proDer p 1, carrying an N-terminal propeptide with a unique structure. The maturation of the zymogen into an enzymatically-active form of Der p 1 is a multistep autocatalytic process initiated under acidic conditions through conformational changes of the propeptide, leading to the loss of its inhibitory ability and its subsequent gradual cleavage. The aims of this study were to characterize the residues present in the Der p 1 propeptide involved in the initiation of the zymogen maturation process, but also to assess the impact of acidic pH on the propeptide structure, the activity of Der p 1 and the fate of the propeptide. Using various complementary enzymatic and structural approaches, we demonstrated that a structural triad K17p-D51p-Y19p within the N-terminal domain of the propeptide is essential for its stabilization and the sensing of pH changes. Particularly, the protonation of D51p under acidic conditions unfolds the propeptide through disruption of the K17p-D51p salt bridge, reduces its inhibition capacity and unmasks the buried residues K17p and Y19p constituting the first maturation cleavage site of the zymogen. Our results also evidenced that this triad acts in a cooperative manner with other propeptide pH-responsive elements, including residues E56p and E80p, to promote the propeptide unfolding and/or to facilitate its proteolysis. Furthermore, we showed that acidic conditions modify Der p 1 proteolytic specificity and confirmed that the formation of the first intermediate represents the limiting step of the in vitro Der p 1 maturation process. Altogether, our results provide new insights into the early events of the mechanism of proDer p 1 maturation and identify a unique structural triad acting as a stabilizing and a pH-sensing regulatory element.

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The Role of the House Dust Mite-Induced Innate Immunity in Development of Allergic Response

Cited by 48 publications

References 182 publications

Effect of <i>1.8-Cineole</i> in <i>Dermatophagoides pteronyssinus</i>-Stimulated Bronchial Epithelial Cells and Mouse Model of Asthma

Effect of <i>1.8-Cineole</i> in <i>Dermatophagoides pteronyssinus</i>-Stimulated Bronchial Epithelial Cells and Mouse Model of Asthma

Polarized Airway Epithelial Models for Immunological Co-Culture Studies

The Lys-Asp-Tyr Triad within the Mite Allergen Der p 1 Propeptide Is a Critical Structural Element for the pH-Dependent Initiation of the Protease Maturation

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