The Role of the Laboratory in the Management of Tuberculosis Patients

Gangadharam, P. R. J.

doi:10.1055/s-2007-1012156

Semin Respir Crit Care Med

1981

DOI: 10.1055/s-2007-1012156

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The Role of the Laboratory in the Management of Tuberculosis Patients

P. R. J. Gangadharam¹

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1982

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Cited by 6 publications

(1 citation statement)

References 19 publications

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“…Currently, sputum smear and culture are used to monitor the response to therapy for patients with pulmonary tuberculosis. Conversion of positive results to negative results after 1 to 2 months of therapy is indicative of effective treatment (7,30). The objective of this work was to determine whether quantitative changes in the amount of mycobacterial DNA present in sputum reflect bactericidal drug activity.…”

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confidence: 99%

Comparison of the ABI 7700 System (TaqMan) and Competitive PCR for Quantification of IS 6110 DNA in Sputum during Treatment of Tuberculosis

et al. 1998

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Mycobacterium tuberculosis can persist in sputum for long periods of time after the initiation of antituberculosis chemotherapy. The purpose of this study was to determine whether quantitative estimates of M. tuberculosis DNA in sputum correlate with the numbers of viable bacilli and thus measure the therapeutic response of patients during treatment. Two methods of M. tuberculosis DNA quantification were examined by using DNA isolated from sputum specimens serially collected during the course of chemotherapy. A competitive PCR assay was compared to an automated system of real-time quantification with the ABI Prism 7700 Sequence Detection System (TaqMan). The ABI 7700 system uses standard PCR in conjunction with a fluorogenic probe in which the intensity of fluorescence is proportional to the amount of target DNA present. The results showed that both PCR systems are reproducible and accurate. The amounts of M. tuberculosis DNA quantified in sputum corresponded well with the numbers of acid-fast bacilli (AFB) counted by microscopy. Before initiation of antituberculosis therapy, measures of AFB, M. tuberculosis DNA, and cultivable bacilli were similar, suggesting that quantification of DNA is a good method for measuring the initial bacillary load. However, the rate of disappearance of both AFB and M. tuberculosis DNA did not correlate with the decline in cultivable bacilli in the specimen; therefore, these tests are not appropriate for monitoring treatment efficacy.

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Comparison of the ABI 7700 System (TaqMan) and Competitive PCR for Quantification of IS 6110 DNA in Sputum during Treatment of Tuberculosis

et al. 1998

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Acridine orange staining of smears for demonstration ofMycobacterium tuberculosis

Katila

Mäntyjärvi

1982

Eur. J, Clin. Microbiol.

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Acridine orange staining for the detection of mycobacteria was compared with staining by auramine O and with mycobacterial culture in a series of 1071 clinical specimens. A total of 78 (7%) specimens were positive by staining. No false positive or negative findings were recorded by the acridine orange method. The two fluorochromes proved equal in their ability to detect mycobacteria in specimens from culture positive cases of tuberculosis. In the rapid bacteriological diagnosis of tuberculosis, acridine orange offers a good alternative to auramine O which is considered carcinogenic.

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A single step culture technique for tubercle bacilli

Vasanthakumari

1990

Tubercle

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The Role of the Laboratory in the Management of Tuberculosis Patients

Cited by 6 publications

References 19 publications

Comparison of the ABI 7700 System (TaqMan) and Competitive PCR for Quantification of IS 6110 DNA in Sputum during Treatment of Tuberculosis

Comparison of the ABI 7700 System (TaqMan) and Competitive PCR for Quantification of IS 6110 DNA in Sputum during Treatment of Tuberculosis

Acridine orange staining of smears for demonstration ofMycobacterium tuberculosis

A single step culture technique for tubercle bacilli

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