The role of the lateral dimension of graphene oxide in the regulation of cellular responses

Yue, Hua; Wei, Wei; Yue, Zhanguo; Wang, Bin; Luo, Nana; Gao, Yongjun; Ma, Ding; Ma, Guanghui; Su, Zhiguo

doi:10.1016/j.biomaterials.2012.02.021

Cited by 354 publications

(309 citation statements)

References 45 publications

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“…GO has smoother edges and hydrophilic properties, and seems to be less potent in terms of penetrating cell compartments and interacting with DNA, resulting in the absence of genotoxic effects. Furthermore, it has been demonstrated that GO particles smaller than 500 nm may be internalized via clathrin-mediated 34 On the other hand, Yue et al 35 showed that 350 nm GO are wrapped by active filopodia of macrophages, while 2 µm GO enter cells nearly perpendicularly. This mechanism may trap GO inside vesicles, making it impossible for GO to penetrate into the nucleus and interact directly with DNA.…”

Section: Discussionmentioning

confidence: 99%

Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells

Hinzmann¹,

Jaworski²,

Kutwin³

et al. 2014

IJN

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The carbon-based nanomaterial family consists of nanoparticles containing allotropes of carbon, which may have a number of interactions with biological systems. The objective of this study was to evaluate the toxicity of nanoparticles comprised of pristine graphene, reduced graphene oxide, graphene oxide, graphite, and ultradispersed detonation diamond in a U87 cell line. The scope of the work consisted of structural analysis of the nanoparticles using transmission electron microscopy, evaluation of cell morphology, and assessment of cell viability by Trypan blue assay and level of DNA fragmentation of U87 cells after 24 hours of incubation with 50 μg/mL carbon nanoparticles. DNA fragmentation was studied using single-cell gel electrophoresis. Incubation with nanoparticles containing the allotropes of carbon did not alter the morphology of the U87 cancer cells. However, incubation with pristine graphene and reduced graphene oxide led to a significant decrease in cell viability, whereas incubation with graphene oxide, graphite, and ultradispersed detonation diamond led to a smaller decrease in cell viability. The results of a comet assay demonstrated that pristine graphene, reduced graphene oxide, graphite, and ultradispersed detonation diamond caused DNA damage and were therefore genotoxic in U87 cells, whereas graphene oxide was not.

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Section: Discussionmentioning

confidence: 99%

Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells

Hinzmann¹,

Jaworski²,

Kutwin³

et al. 2014

IJN

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“…The cytotoxicity strongly depends on the type of graphene materials as well as on physical and chemical properties and the type and level of chemical functionalization. 10,14,16,24,44,95,96 Figure 9 effect of gO, rgO, rgO-ag nanocomposite, and agNPs on the leakage of lDh to culture supernatant of human ovarian cancer cells. Notes: lDh activity is measured at 490 nm using the lDh cytotoxicity kit.…”

Section: Effect Of Rgo-ag Nanocomposite On Membrane Integritymentioning

confidence: 99%

Reduced graphene oxide–silver nanoparticle nanocomposite: a potential anticancer nanotherapy

Gurunathan¹,

Han²,

Park³

et al. 2015

IJN

233

218

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Background Graphene and graphene-based nanocomposites are used in various research areas including sensing, energy storage, and catalysis. The mechanical, thermal, electrical, and biological properties render graphene-based nanocomposites of metallic nanoparticles useful for several biomedical applications. Epithelial ovarian carcinoma is the fifth most deadly cancer in women; most tumors initially respond to chemotherapy, but eventually acquire chemoresistance. Consequently, the development of novel molecules for cancer therapy is essential. This study was designed to develop a simple, non-toxic, environmentally friendly method for the synthesis of reduced graphene oxide–silver (rGO–Ag) nanoparticle nanocomposites using Tilia amurensis plant extracts as reducing and stabilizing agents. The anticancer properties of rGO–Ag were evaluated in ovarian cancer cells. Methods The synthesized rGO–Ag nanocomposite was characterized using various analytical techniques. The anticancer properties of the rGO–Ag nanocomposite were evaluated using a series of assays such as cell viability, lactate dehydrogenase leakage, reactive oxygen species generation, cellular levels of malonaldehyde and glutathione, caspase-3 activity, and DNA fragmentation in ovarian cancer cells (A2780). Results AgNPs with an average size of 20 nm were uniformly dispersed on graphene sheets. The data obtained from the biochemical assays indicate that the rGO–Ag nanocomposite significantly inhibited cell viability in A2780 ovarian cancer cells and increased lactate dehydrogenase leakage, reactive oxygen species generation, caspase-3 activity, and DNA fragmentation compared with other tested nanomaterials such as graphene oxide, rGO, and AgNPs. Conclusion T. amurensis plant extract-mediated rGO–Ag nanocomposites could facilitate the large-scale production of graphene-based nanocomposites; rGO–Ag showed a significant inhibiting effect on cell viability compared to graphene oxide, rGO, and silver nanoparticles. The nanocomposites could be effective non-toxic therapeutic agents for the treatment of both cancer and cancer stem cells.

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“…However, the underlying mechanisms of cellular internalization of GBMs remain enigmatic and several pathways have been proposed. The two main working hypotheses include phagocytosis and clathrin-mediated endocytosis 30,31 but the possibility of membrane translocation through a "piercing effect" has also been revealed from computational studies 32,33 . Remarkably, photothermal effect due to the ability of graphene to absorb NIR light was suggested to enhance the transfection efficiency thanks to induced heating which locally disrupts the organization of the lipid bilayer cell membrane, hence rendering it more permeable and facilitating endosomal escape 34 .…”

Section: What Can Gbms Offer As Gene Delivery Platforms?mentioning

confidence: 99%

Graphene materials as 2D non-viral gene transfer vector platforms

2016

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Advances in genomics and gene therapy could offer solutions to many diseases that remain incurable today, however one of the critical reasons halting clinical progress is due to the difficulty in designing efficient and safe delivery vectors for the appropriate genetic cargo. Safety and largescale production concerns counter-balance the high gene transfer efficiency achieved with viral vectors, while non-viral strategies have yet to become sufficiently efficient. The extraordinary physicochemical, optical and photothermal properties of graphene-based materials (GBMs) could offer two-dimensional (2D) components for the design of nucleic acid carrier systems. We discuss here such properties and their implications for the optimization of gene delivery. While the design of such vectors is still in its infancy, we provide here an exhaustive and up-to-date analysis of the studies that have explored GBMs as gene transfer vectors, focusing on the functionalization strategies followed to improve vector performance and on the biological effects attained.

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The role of the lateral dimension of graphene oxide in the regulation of cellular responses

Cited by 354 publications

References 45 publications

Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells

Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells

Reduced graphene oxide–silver nanoparticle nanocomposite: a potential anticancer nanotherapy

Graphene materials as 2D non-viral gene transfer vector platforms

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The role of the lateral dimension of graphene oxide in the regulation of cellular responses

Cited by 354 publications

References 45 publications

Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells

Nanoparticles containing allotropes of carbon have genotoxic effects on glioblastomamultiforme cells

Reduced graphene oxide&ndash;silver nanoparticle nanocomposite: a potential anticancer nanotherapy

Graphene materials as 2D non-viral gene transfer vector platforms

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Reduced graphene oxide–silver nanoparticle nanocomposite: a potential anticancer nanotherapy