The Role of the Microenvironment in Controlling the Fate of Bioprinted Stem Cells

West-Livingston, Lauren; Park, Jihoon; Lee, Sang Jin; Atala, Anthony; Yoo, James J.

doi:10.1021/acs.chemrev.0c00126

Cited by 54 publications

(32 citation statements)

References 317 publications

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“…Additionally, extracellular matrix (ECM)-derived bioinks have been utilized to accelerate the tissue maturation and formation in the bioprinted tissue constructs [21,22]. These ECMderived bioinks may retain tissue-specific structural and functional molecules that regulate cell behavior and tissue homeostasis [21,23,24].…”

Section: Introductionmentioning

confidence: 99%

A photo-crosslinkable cartilage-derived extracellular matrix bioink for auricular cartilage tissue engineering

et al. 2021

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Three-dimensional (3D) bioprinting of patient-specific auricular cartilage constructs would aid in the reconstruction process of traumatically injured or congenitally deformed ear cartilage. To achieve this, a hydrogel-based bioink is required that recapitulates the complex cartilage microenvironment. Tissue-derived decellularized extracellular matrix (dECM)-based hydrogels have been used as bioinks for cell-based 3D bioprinting because they contain tissue-specific ECM components that play a vital role in cell adhesion, growth, and differentiation. In this study, porcine auricular cartilage tissues were isolated and decellularized, and the decellularized cartilage tissues were characterized by histology, biochemical assay, and proteomics. This cartilage-derived dECM (cdECM) was subsequently processed into a photo-crosslinkable hydrogel using methacrylation (cdECMMA) and mixed with chondrocytes to create a printable bioink. The rheological properties, printability, and in vitro biological properties of the cdECMMA bioink were examined. The results showed cdCEM was obtained with complete removal of cellular components while preserving major ECM proteins. After methacrylation, the cdECMMA bioinks were printed in anatomical ear shape and exhibited adequate mechanical properties and structural integrity. Specifically, auricular chondrocytes in the printed cdECMMA hydrogel constructs maintained their viability and proliferation capacity and eventually produced cartilage ECM components, including collagen and glycosaminoglycans (GAGs). The potential of cell-based bioprinting using this cartilage-specific dECMMA bioink is demonstrated as an alternative option for auricular cartilage reconstruction.

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Section: Introductionmentioning

confidence: 99%

A photo-crosslinkable cartilage-derived extracellular matrix bioink for auricular cartilage tissue engineering

et al. 2021

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“…However, scientists have investigated the microenvironment of stem cells in bone marrow at single cell level 22 . Tissue engineering technology could reproduce the microenvironment on which stem cells depend and improve the e cacy of therapy 23 . "Microtissue engineering" is a research eld that has emerged to address abovementioned challenges, aiming to construct a precise and controllable cell "microenvironment" with bionic structures and functions on a "microscale" 24,25 .…”

Section: Discussionmentioning

confidence: 99%

The 100 Most Cited Articles on the Applications of Stem Cells in Osteoarthritis: A Bibliometric Analysis

Yang¹,

Zhou²,

Lin

2020

Preprint

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Background: Stem cells have been applied in the treatment of OA, which had attracted wide attention. However, the research area is relatively extensive, and the research level is variable. In this study, we reviewed the mechanisms and clinical applications of stem cells in OA by using bibliometric analysis for the first time. We also revealed the characteristics, superior results and developmental trends in this field.Methods: The Web of Science core collection database was used to search articles related to the application of stem cells in OA. We collected the general information from the top 100 cited articles. We analyzed and evaluated the articles according to publication number, journals, institutions, countries, keywords and extended keywords.Results: The 100 most cited articles were cited from 129 to 1353 times mainly reviews and original articles. These articles were published from 2001 to 2017 and distributed evenly in America, East Asia and European countries. The United States contributed most in published number and international cooperation. The top ten institutions are mainly major universities and Duke University published a maximum of 10 articles. In terms of journals ,57 articles were published in the top ten journals. The keywords were divided into 8 categories from molecular mechanisms to clinical application.Conclusions: In our study, we found that mesenchymal stem cells (MSCs) which could repair articular cartilage and inhibit local inflammation, are the most widely applied in research and treatment of OA. TGF-βwas crucial during the process. Exosomes are regarded as the active ingredients in stem cell therapy for OA. Microtissue engineering will contribute to accurate and effective stem cell therapy. The findings of our study will contribute to the continuous development of research and direct the research of stem cells in OA.

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“…Its microenvironment influences progenitor cell proliferation in different directions, including neuronal differentiation, liver, myogenic, chondrogenic, osteogenic, adipogenic, and cardiogenic cells. 31 The growth of progenitor cells is regulated by both circulating and local signaling pathways. These multiple regulatory pathways, including receptor tyrosine kinases (RTKs), Notch (N), Hedgehog (Hh), TGFβ, Jak/Stat, nuclear receptors, and Wnt, are involved in cell differentiation.…”

Section: Wnt Signaling Pathway and Embryonic Cardiac Developmentmentioning

confidence: 99%

Roles of Wnt Signaling Pathway and ROR2 Receptor in Embryonic Development: An Update Review Article

Guo

Xing

2022

Genet Epigenet

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The Wnt family is a large class of highly conserved cysteine-rich secretory glycoproteins that play a vital role in various cellular and physiological courses through different signaling pathways during embryogenesis and tissue homeostasis 3. Wnt5a is a secreted glycoprotein that belongs to the noncanonical Wnt family and is involved in a wide range of developmental and tissue homeostasis. A growing body of evidence suggests that Wnt5a affects embryonic development, signaling through various receptors, starting with the activation of β-catenin by Wnt5a. In addition to affecting planar cell polarity and Ca2+ pathways, β-catenin also includes multiple signaling cascades that regulate various cell functions. Secondly, Wnt5a can bind to Ror receptors to mediate noncanonical Wnt signaling and a significant ligand for Ror2 in vertebrates. Consistent with the multiple functions of Wnt5A/Ror2 signaling, Wnt5A knockout mice exhibited various phenotypic defects, including an inability to extend the anterior and posterior axes of the embryo. Numerous essential roles of Wnt5a/Ror2 in development have been demonstrated. Therefore, Ror signaling pathway become a necessary target for diagnosing and treating human diseases. The Wnt5a- Ror2 signaling pathway as a critical factor has attracted extensive attention.

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The Role of the Microenvironment in Controlling the Fate of Bioprinted Stem Cells

Cited by 54 publications

References 317 publications

A photo-crosslinkable cartilage-derived extracellular matrix bioink for auricular cartilage tissue engineering

A photo-crosslinkable cartilage-derived extracellular matrix bioink for auricular cartilage tissue engineering

The 100 Most Cited Articles on the Applications of Stem Cells in Osteoarthritis: A Bibliometric Analysis

Roles of Wnt Signaling Pathway and ROR2 Receptor in Embryonic Development: An Update Review Article

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