The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis

Baxley, Ryan M.; Soshnev, Alexey A.; Koryakov, Dmitry E.; Жимулев, И. Ф.; Geyer, Pamela

doi:10.1016/j.ydbio.2011.05.666

Cited by 50 publications

(139 citation statements)

References 77 publications

(119 reference statements)

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“…Chromosome association of Su(Hw), CP190 and Mod67.2 was tested using ChIP from 50-200 ovary pairs per biological sample, as described previously (Baxley et al, 2011). The antibodies used were guinea pig polyclonal anti-Su(Hw) (Baxley et al, 2011;Soshnev et al, 2012), sheep polyclonal anti-CP190 (Baxley et al, 2011) and rabbit polyclonal antiMod67.2 (modEncode D1; kindly provided by K. White, University of Chicago).…”

Section: Chromatin Immunoprecipitation (Chip)mentioning

confidence: 99%

“…The antibodies used were guinea pig polyclonal anti-Su(Hw) (Baxley et al, 2011;Soshnev et al, 2012), sheep polyclonal anti-CP190 (Baxley et al, 2011) and rabbit polyclonal antiMod67.2 (modEncode D1; kindly provided by K. White, University of Chicago). At least two independent biological samples were analyzed.…”

Section: Chromatin Immunoprecipitation (Chip)mentioning

confidence: 99%

“…Slides were imaged using a Zeiss 710 confocal microscope and processed using ImageJ. The antibodies used were polyclonal guinea pig anti-Su(Hw) (1:500) (Baxley et al, 2011), polyclonal rabbit anti-Su(Hw) (1:1000) (Parnell et al, 2003), rat anti-ELAV [1:10; 7E8A10; Developmental Studies Hybridoma Bank (DSHB), University of Iowa], mouse anti-Broad Z1 (1:200; Z1.3C11.OA1; DSHB), mouse anti-Repo (1:5; 8D12; DSHB), mouse antiGurken (1:200; 1D12; DSHB), polyclonal rabbit anti-Vasa (1:500; Santa Cruz), polyclonal guinea pig anti-Dpn (1:500; a kind gift from J. Skeath, Washington University, St Louis) and polyclonal rabbit anti-Rbp9 (1:3000; a kind gift from M. Buszczak, UT Southwestern) (Kim and Baker, 1993).…”

Section: Immunohistochemical Analysesmentioning

confidence: 99%

“…Even so, loss of Su(Hw) causes a tissue-restricted phenotype, wherein oogenesis is defective owing to oocyte apoptosis during mid-oogenesis (Klug et al, 1968;Parkhurst et al, 1988;Harrison et al, 1993;Baxley et al, 2011). Two observations suggest that the fertility and insulator functions of Su(Hw) are independent.…”

Section: Introductionmentioning

confidence: 99%

“…First, a loss of Su(Hw) occupancy at ~60% of genomic SBSs has no effect on fertility (Soshnev et al, 2012), an unexpected finding for an insulator-dependent function that has been linked to the formation of physical chromatin domains important for transcriptional regulation (Gurudatta and Corces, 2009;Yang and Corces, 2011;Hou et al, 2012). Second, fertility is unaffected by loss of CP190 and Mod67.2 (Chodagam et al, 2005;Baxley et al, 2011), two partners that are required for Su(Hw) insulator function. Together, these findings suggest that Su(Hw) functions in oogenesis extend beyond the formation of chromatin insulators.…”

Section: Introductionmentioning

confidence: 99%

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The insulator protein Suppressor of Hairy-wing is an essential transcriptional repressor in theDrosophilaovary

et al. 2013

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SUMMARYSuppressor of Hairy-wing [Su(Hw)] is a DNA-binding factor required for gypsy insulator function and female germline development in Drosophila. The insulator function of the gypsy retrotransposon depends on Su(Hw) binding to clustered Su(Hw) binding sites (SBSs) and recruitment of the insulator proteins Centrosomal Protein 190 kD (CP190) and Modifier of mdg4 67.2 kD (Mod67.2). By contrast, the Su(Hw) germline function involves binding to non-clustered SBSs and does not require CP190 or Mod67.2. Here, we identify Su(Hw) target genes, using genome-wide analyses in the ovary to uncover genes with an ovary-bound SBS that are misregulated upon Su(Hw) loss. Most Su(Hw) target genes demonstrate enriched expression in the wild-type CNS. Loss of Su(Hw) leads to increased expression of these CNS-enriched target genes in the ovary and other tissues, suggesting that Su(Hw) is a repressor of neural genes in non-neural tissues. Among the Su(Hw) target genes is RNA-binding protein 9 (Rbp9), a member of the ELAV/Hu gene family. Su(Hw) regulation of Rbp9 appears to be insulator independent, as Rbp9 expression is unchanged in a genetic background that compromises the functions of the CP190 and Mod67.2 insulator proteins, even though both localize to Rbp9 SBSs.

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Section: Chromatin Immunoprecipitation (Chip)mentioning

confidence: 99%

Section: Chromatin Immunoprecipitation (Chip)mentioning

confidence: 99%

Section: Immunohistochemical Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The insulator protein Suppressor of Hairy-wing is an essential transcriptional repressor in theDrosophilaovary

et al. 2013

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Expanding the roles of chromatin insulators in nuclear architecture, chromatin organization and genome function

Schoborg

Labrador

2014

Cell. Mol. Life Sci.

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Of the numerous classes of elements involved in modulating eukaryotic chromosome structure and function, chromatin insulators arguably remain the most poorly understood in their contribution to these processes in vivo. Indeed, our view of chromatin insulators has evolved dramatically since their chromatin boundary and enhancer blocking properties were elucidated roughly a quarter of a century ago as a result of recent genome-wide, high-throughput methods better suited to probing the role of these elements in their native genomic contexts. The overall theme that has emerged from these studies is that chromatin insulators function as general facilitators of higher-order chromatin loop structures that exert both physical and functional constraints on the genome. In this review, we summarize the result of recent work that supports this idea as well as a number of other studies linking these elements to a diverse array of nuclear processes, suggesting that chromatin insulators exert master control over genome organization and behavior.

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Analysis of a rare progeria variant of Barrier-to-autointegration factor in Drosophila connects centromere function to tissue homeostasis

Duan

Srikantha

Amoiroglou

et al. 2023

Cell. Mol. Life Sci.

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Barrier-to-autointegration factor (BAF/BANF) is a nuclear lamina protein essential for nuclear integrity, chromatin structure, and genome stability. Whereas complete loss of BAF causes lethality in multiple organisms, the A12T missense mutation of the BANF1 gene in humans causes a premature aging syndrome, called Néstor-Guillermo Progeria Syndrome (NGPS). Here, we report the first in vivo animal investigation of progeroid BAF, using CRISPR editing to introduce the NGPS mutation into the endogenous Drosophila baf gene. Progeroid BAF adults are born at expected frequencies, demonstrating that this BAF variant retains some function. However, tissue homeostasis is affected, supported by studies of the ovary, a tissue that depends upon BAF for stem cell survival and continuous oocyte production. We find that progeroid BAF causes defects in germline stem cell mitosis that delay anaphase progression and compromise chromosome segregation. We link these defects to decreased recruitment of centromeric proteins of the kinetochore, indicating dysfunction of cenBAF, a localized pool of dephosphorylated BAF produced by Protein Phosphatase PP4. We show that DNA damage increases in progenitor germ cells, which causes germ cell death due to activation of the DNA damage transducer kinase Chk2. Mitotic defects appear widespread, as aberrant chromosome segregation and increased apoptosis occur in another tissue. Together, these data highlight the importance of BAF in establishing centromeric structures critical for mitosis. Further, these studies link defects in cenBAF function to activation of a checkpoint that depletes progenitor reserves critical for tissue homeostasis, aligning with phenotypes of NGPS patients.

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The role of the Suppressor of Hairy-wing insulator protein in Drosophila oogenesis

Cited by 50 publications

References 77 publications

The insulator protein Suppressor of Hairy-wing is an essential transcriptional repressor in theDrosophilaovary

The insulator protein Suppressor of Hairy-wing is an essential transcriptional repressor in theDrosophilaovary

Expanding the roles of chromatin insulators in nuclear architecture, chromatin organization and genome function

Analysis of a rare progeria variant of Barrier-to-autointegration factor in Drosophila connects centromere function to tissue homeostasis

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