The role of ThinPrep cytology in the investigation of lung tumors

Konofaos, Petros; Tomos, Periklis; Malagari, Katerina; Karakatsani, Anna; Pavlopoulos, Dionisios; Lachanas, Elias; Flessas, M.; Kostakis, A.; Karakitsos, Petros

doi:10.1016/j.suronc.2006.12.001

Cited by 21 publications

(26 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Konofaos et al compared TP with CP in lung FNA in a split sample study (ie, aspirate ''split'' to prepare both TP and CP). 11 They studied 80 cases and found that TP outperformed CP in regard to overall accuracy and that the nondiagnostic rate was lower in TP. Only 3 of 80 cases in their study were SCCL, and the authors noted in their discussion that additional studies were necessary to confirm their belief that TP cytology is a sound modality to differentiate SCCL from other lung tumors in the differential.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of ThinPrep cytology in establishing the diagnosis of small cell carcinoma of lung

Kim

Owens

2009

Cancer Cytopathology

View full text Add to dashboard Cite

BACKGROUND:ThinPrep liquid‐based cytology (TP) preparations are being used increasingly in nongynecologic specimens. To the authors' knowledge, few studies to date have evaluated TP cytology in the setting of small cell carcinoma of the lung (SCCL). Accurately differentiating SCCL from other lung tumors has important clinical and therapeutic implications. Herein, the authors evaluated the diagnostic utility and cytomorphology of TP in the setting of SCCL.METHODS:All cases of SCCL with prior or concurrent TP cytologic specimens were identified via computer search. The cytodiagnoses were tabulated. When available, cytologic material was reviewed. Performance parameters of the various cytologic modalities processed with TP were calculated.RESULTS:In 121 patients with SCCL, 261 TP specimens were identified. The cytodiagnoses were: SCCL (119 specimens), suspicious for SCCL (45 specimens), atypical cells‐not otherwise specified (22 specimens), negative/nondiagnostic (63 specimens), and nonsmall cell carcinoma (4 specimens). During the same period of time, 3 cases of false‐positive diagnoses of SCCL were identified. The positive predictive value for a cytodiagnosis of SCCL on TP was 97.5%, and the sensitivity was 62.8%. Bronchial brush was the most sensitive cytologic modality (78.3%). Immunostaining was found to be contributory to the diagnosis in 10 of the 11 cases in which it was attempted.CONCLUSIONS:TP is a sound alternative to conventional preparations for the cytodiagnosis of SCCL. Cytomorphology of SCCL is altered in TP with less molding, less cell fragility, more discohesion, and tumor cell shrinkage artifact. Immunohistochemical staining of cellblocks is a useful adjunct in challenging cases. A positive cytodiagnosis of SCCL on TP can be used to initiate definitive therapy when biopsy is not possible. Cancer (Cancer Cytopathol) 2009. © 2009 American Cancer Society.

show abstract

Section: Discussionmentioning

confidence: 99%

“…2,9,10 The utility of TP in lung cytology has been addressed in few studies, and in these studies only a small number of cases were small cell carcinoma of lung (SCCL). [11][12][13] Thus, the utility and limitations of TP in the cytologic diagnosis of SCCL is still evolving. Accurately diagnosing SCCL is critical for subsequent therapeutic decisions.…”

mentioning

confidence: 99%

Analysis of ThinPrep cytology in establishing the diagnosis of small cell carcinoma of lung

Kim

Owens

2009

Cancer Cytopathology

View full text Add to dashboard Cite

show abstract

“…Unfortunately, due to budget and staff limitation, rapid on-site evaluation is not always feasible [5,6,7]. In this setting, liquid-based cytology (LBC) represents a valid alternative to traditional cytology, avoiding the possibility that untrained clinicians may improperly smear and triage the aspirated material, thus limiting artifacts [8,9]. …”

Section: Introductionmentioning

confidence: 99%

Epidermal Growth Factor Receptor Test Performed on Liquid-Based Cytology Lung Samples: Experience of an Academic Referral Center

Bellevicine

Malapelle²,

Vigliar³

et al. 2014

Acta Cytologica

View full text Add to dashboard Cite

Objectives: In this study we reviewed our practice of lung cancer epidermal growth factor receptor (EGFR) mutational testing in an academic centralized laboratory setting, where direct smears and liquid-based cytology (LBC) slides represent the most frequent cytological specimens received. The aim was to assess the differences, if any, between these sample types in terms of DNA yield, adequacy rates and overall EGFR testing performance. Study Design: A total of 362 cases were retrieved - received from January 2012 to January 2014 for EGFR testing - including 204 LBC specimens and 158 smears. Exon 19 deletions and the L858R point mutation in exon 21, detected by fragment assay and TaqMan assay, respectively, were confirmed by direct sequencing or by high-resolution melting. Results: Although the direct smears showed a higher DNA yield (60.94 vs. 23.07 ng/µl) and were more frequently cell-rich (54%) than the LBC slides (31.4%), the differences in adequacy (direct smears: 97.4%; LBCs: 94.1%) and in mutant rate (direct smears: 10.3%; LBCs: 14.0%) between the two sample types did not reach statistical significance. Conclusions: Not only direct smears but also LBC slides represent an effective preparation and storage medium for cytological material to be used for EGFR molecular testing.

show abstract

“…Rana et al [3] reported the detection of 19 SqCC by TP versus 16 by CP, while both techniques equally detected ADC and small cell carcinoma (SmCC). Despite the use of residual rinse for TP, Konofaos et al [10] reported the detection of 50/53 SqCC by TP versus 36 by CP and 16/18 versus 12/18 for ADC, respectively. Both techniques detected 3/3 SmCC and 2/3 hamartomas.…”

Section: Resultsmentioning

confidence: 99%

“…Nuclear molding was the most prominent criterion distinguishing SmCC from other small round cell tumors. Two other studies reported the detection of 3/3 and 8/8 SmCC, respectively [10,12]. Kim et al [13] reported a positive predictive value of 97.5% with 3 false-positive cases (2 basaloid SqCC and 1 atypical carcinoid) and 2 false-negative cases that were retrospectively diagnosed as suspicious for SmCC.…”

Section: Resultsmentioning

confidence: 99%

The Implementation of Liquid-Based Cytology for Lung and Pleural-Based Diseases

Michael

Bedrossian²

2014

Acta Cytologica

View full text Add to dashboard Cite

Objective: First introduced for the processing of cervico-vaginal samples, liquid-based cytology (LBC) soon found application in nongynecological specimens, including bronchoscopic brushings, washings and transcutaneous and transbronchial aspiration biopsy of the lung as well as pleural effusions. This article reviews the existing literature related to these specimens along with the authors' own experience. Study Design: A literature review was conducted through Ovid MEDLINE and PubMed search engines using several key words. Results: Most of the literature is based on data collected through the use of split samples. The data confirms that the use of LBC is an acceptable, and sometimes superior, alternative to the conventional preparations (CP). LBC offers several advantages, including the ability to transport in a stable collecting media, elimination of obscuring elements, ease of screening, excellent preservation, random representative sample, and application of ancillary techniques on additional preparations. Some diagnostic pitfalls related to the introduced artifacts were reported. Conclusion: The utilization of LBC offers many advantages over CP and has a diagnostic accuracy that is equal to or surpasses that of CP. LBC affords a bridge to the future application of molecular and other ancillary techniques to cytology. Knowledge of the morphological artifacts is useful at the early stages of implementation.

show abstract

The role of ThinPrep cytology in the investigation of lung tumors

Cited by 21 publications

References 14 publications

Analysis of ThinPrep cytology in establishing the diagnosis of small cell carcinoma of lung

Analysis of ThinPrep cytology in establishing the diagnosis of small cell carcinoma of lung

Epidermal Growth Factor Receptor Test Performed on Liquid-Based Cytology Lung Samples: Experience of an Academic Referral Center

The Implementation of Liquid-Based Cytology for Lung and Pleural-Based Diseases

Contact Info

Product

Resources

About