The Role of Tinospora cordifolia in Extenuating the Toxic Effects of CPF on Hepatic Modifications along with Electron Microscope observations in Red Blood Cells

Abstract

This study investigates the role of Tinospora cordifolia (TC) extract against liver damage induced in male albino rat by an Organophosphorus (OP) insecticide, Chloropyrifos (CPF). Liver damage was investigated by various parameters like Lipid peroxidation (LPO), Superoxide dismutase (SOD), Catalase, Glutathione reductase (GR), Lactate dehydrogenase (LDH), Acid phosphatase, Na + /K + -ATPase and Acetyl cholinesterase (AChE) and Scanning Electron microscope (SEM) observation on blood cells. Group I Control group, Group II 30 mg/kg CPF b. wt., Group III TC 1.0 g/kg b. wt. with 30 mg/kg CPF b. wt., Group IV TC 2.0 g/kg b.wt. with 30 mg/kg CPF b. wt., Group V TC 4.0 g/kg b.wt. with 30 mg/kg CPF b. wt. and Group VI 4.0 g/kg b.wt. of TC were administered orally via gavages. The results showed that TC overcome the CPF induced decreased levels of SOD, Catalase, Glutathione reductase, LDH, Acid Phosphatase, Na + /K + -ATPase and AChE compare to CPF control. Moreover, TC significantly decreased the LPO level induced by CPF as compared to CPF control. The drastic alterations in the shape of the blood cells were reverted significantly with the increasing doses concentration of TC. Therefore, it could be concluded that TC administration is useful in routine therapy for protection against tissue damage induced by CPF.