HighlightsThe potential of fusing GF-1 WFV and MODIS data by the ESTARFM algorithm was demonstrated.A better time window selection method for estimating yields was provided.A better vegetation index suitable for yield estimation based on spatiotemporally fused data was identified.The effect of the spatial resolution of remote sensing data on yield estimations was visualized.Abstract. The accurate estimation of crop yields is very important for crop management and food security. Although many methods have been developed based on single remote sensing data sources, advances are still needed to exploit multisource remote sensing data with higher spatial and temporal resolution. More suitable time window selection methods and vegetation indexes, both of which are critical for yield estimations, have not been fully considered. In this article, the Chinese GaoFen-1 Wide Field View (GF-1 WFV) and Terra Moderate Resolution Imaging Spectroradiometer (MODIS) data were fused by the Enhanced Spatial and Temporal Adaptive Reflectance Fusion Model (ESTARFM) to generate time-series data with a high spatial resolution. Then, two time window selection methods involving distinguishing or not distinguishing the growth stages during the monitoring period, and three vegetation indexes, the normalized difference vegetation index (NDVI), two-band enhanced vegetation index (EVI2) and wide dynamic range vegetation index (WDRVI), were intercompared. Furthermore, the yield estimations obtained from two different spatial resolutions of fused data and MODIS data were analyzed. The results indicate that taking the growth stage as the time window unit division basis can allow a better estimation of winter wheat yield; and that WDRVI is more suitable for yield estimations than NDVI or EVI2. This study demonstrates that the spatial resolution has a great influence on yield estimations; further, this study identifies a better time window selection method and vegetation index for improving the accuracy of yield estimations based on a multisource remote sensing data fusion. Keywords: Remote sensing, Spatiotemporal data fusion, Winter wheat, Yield estimation.