The Role of Topoisomerase II in DNA Repair and Recombination in Arabidopsis thaliana

Martinez-Garcia, Marina; White, Charles I.; Franklin, F. Christopher H.; Sanchez‐Moran, Eugenio

doi:10.3390/ijms222313115

Cited by 9 publications

(3 citation statements)

References 85 publications

(129 reference statements)

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“…The finding that variation in TOPII could affect CO frequency is consistent with the results of a previous study in yeast 10 and demonstrates that the TOPII function in wheat and yeast is conserved. This observation is different from that in Arabidopsis 11 , where no detectable differences in CO rate were found between the mutant and wild-type variants of TOPII. This could be associated with differences in the meiotic process in Arabidopsis and wheat, which have different genome organization, ploidy levels, and more than 100-fold difference in genome size.…”

Section: Discussioncontrasting

confidence: 99%

“…In yeast, topoisomerase II (TOPII) regulates the distribution and frequency of COs, with the reduction of TOP II activity being associated with an increased CO rate 10 . Contrary to these trends, the patterns of CO distribution and their count in the top II mutants of Arabidopsis were not significantly different from wild-type genotypes 11 , suggesting that the role of TOPII in CO formation in plants and yeast could differ. However, the genetic mapping of meiotic genes in wheat points to a possible association between TOPII and natural variation in CO count 12 .…”

Section: Introductioncontrasting

confidence: 63%

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Allelic variation in the wheat homolog of topoisomeraseIIis associated with crossover rate

Jordan

Soto

Korol

et al. 2023

Preprint

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Meiotic recombination is a fundamental biological process that impacts genetic diversity and response of variants to selection. The increase in crossover (CO) frequency by modulating the activity of meiotic genes has been suggested as potential means to improve the efficiency of selection and mitigate the negative impact of linkage drag in crop improvement programs. In this study, we used the wheat nested association mapping (NAM) population to map QTL associated with CO rate and harboring TaTOPII-A1, a gene encoding topoisomerase II that is involved in the regulation of meiosis. An amino acid changing SNP (T/C) located in exon 19 of TaTOPII-A1 with a predicted functional effect showed an association with CO rate. The T-allele of TaTOPII-A1 was associated with a statistically significant 5.9 % increase in COs across several NAM families. The involvement of TaTOPII-A1 in processes affecting CO rate in wheat was confirmed using a TaTOPII-A1 mutant. A strong-effect missense mutation in the TaTOPII-A1 coding region resulted in 1.4-fold increase in the genetic map length and 53% increase in CO rate compared to the wild-type allele. These results demonstrate that functional mutations in TaTOPII-A1 can lead to increased CO frequency. The mutant and natural alleles of TaTOPII-A1 identified in our study could serve as new sources of recombination-promoting genes to manipulate CO rate in wheat and possibly other crops.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductioncontrasting

confidence: 63%

Allelic variation in the wheat homolog of topoisomeraseIIis associated with crossover rate

Jordan

Soto

Korol

et al. 2023

Preprint

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show abstract

“…The following supporting information can be downloaded at . References [ 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , …”

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Whole-Genome Sequencing and Analysis of Tumour-Forming Radish (Raphanus sativus L.) Line

Kuznetsova,

Dodueva,

Afonin

et al. 2024

IJMS

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Spontaneous tumour formation in higher plants can occur in the absence of pathogen invasion, depending on the plant genotype. Spontaneous tumour formation on the taproots is consistently observed in certain inbred lines of radish (Raphanus sativus var. radicula Pers.). In this paper, using Oxford Nanopore and Illumina technologies, we have sequenced the genomes of two closely related radish inbred lines that differ in their ability to spontaneously form tumours. We identified a large number of single nucleotide variants (amino acid substitutions, insertions or deletions, SNVs) that are likely to be associated with the spontaneous tumour formation. Among the genes involved in the trait, we have identified those that regulate the cell cycle, meristem activity, gene expression, and metabolism and signalling of phytohormones. After identifying the SNVs, we performed Sanger sequencing of amplicons corresponding to SNV-containing regions to validate our results. We then checked for the presence of SNVs in other tumour lines of the radish genetic collection and found the ERF118 gene, which had the SNVs in the majority of tumour lines. Furthermore, we performed the identification of the CLAVATA3/ESR (CLE) and WUSCHEL (WOX) genes and, as a result, identified two unique radish CLE genes which probably encode proteins with multiple CLE domains. The results obtained provide a basis for investigating the mechanisms of plant tumour formation and also for future genetic and genomic studies of radish.

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The plant early recombinosome: a high security complex to break DNA during meiosis

Rafiei,

Ronceret

2024

Plant Reprod

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Key message The formacion of numerous unpredictable DNA Double Strand Breaks (DSBs) on chromosomes iniciates meiotic recombination. In this perspective, we propose a ‘multi-key lock’ model to secure the risky but necesary breaks as well as a ‘one per pair of cromatids’ model for the topoisomerase-like early recombinosome. Abstract During meiosis, homologous chromosomes recombine at few sites of crossing-overs (COs) to ensure correct segregation. The initiation of meiotic recombination involves the formation of DNA double strand breaks (DSBs) during prophase I. Too many DSBs are dangerous for genome integrity: if these DSBs are not properly repaired, it could potentially lead to chromosomal fragmentation. Too few DSBs are also problematic: if the obligate CO cannot form between bivalents, catastrophic unequal segregation of univalents lead to the formation of sterile aneuploid spores. Research on the regulation of the formation of these necessary but risky DSBs has recently advanced in yeast, mammals and plants. DNA DSBs are created by the enzymatic activity of the early recombinosome, a topoisomerase-like complex containing SPO11. This opinion paper reviews recent insights on the regulation of the SPO11 cofactors necessary for the introduction of temporally and spatially controlled DSBs. We propose that a ‘multi-key-lock’ model for each subunit of the early recombinosome complex is required to secure the formation of DSBs. We also discuss the hypothetical implications that the established topoisomerase-like nature of the SPO11 core-complex can have in creating DSB in only one of the two replicated chromatids of early prophase I meiotic chromosomes. This hypothetical ‘one per pair of chromatids’ DSB formation model could optimize the faithful repair of the self-inflicted DSBs. Each DSB could use three potential intact homologous DNA sequences as repair template: one from the sister chromatid and the two others from the homologous chromosomes.

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The Role of Topoisomerase II in DNA Repair and Recombination in Arabidopsis thaliana

Cited by 9 publications

References 85 publications

Allelic variation in the wheat homolog of topoisomeraseIIis associated with crossover rate

Allelic variation in the wheat homolog of topoisomeraseIIis associated with crossover rate

Whole-Genome Sequencing and Analysis of Tumour-Forming Radish (Raphanus sativus L.) Line

The plant early recombinosome: a high security complex to break DNA during meiosis

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