The Role of Transcription Factors Dfoxo, Dsir2 and Hsp70 in Lifespan Alteration of Drosophila Melanogaster in Different Light Conditions

Abstract

ВВЕДЕНИЕВ настоящее время активно исследуется влияние на продолжительность жизни (ПЖ) различных экологических факторов -температуры (Helfand, Rogina, 2003;Huang et al., 2004), качества пищи (Helfand, Rogina 2003;Cheng et al., 2005;Partridge et al., 2005), ионизирующей радиации (Моска-лев, 2004Sasaki, Fukuda, 2006;Moskalev, 2007;Moskalev et al., 2008), ведется поиск генетических механизмов их влияния на скорость старения ор-ганизма. В то же время работы, касающиеся проблем генетического контроля влияния на ПЖ различных режимов освещения, крайне немногочисленны.Известно, что искусственное увеличение длины светового дня уменьшает ПЖ Drosophila melanogaster и мышей, тогда как его укорочение увеличива-ет ее (Москалев и др

The genetic mechanisms of the influence of the light regime on the lifespan of Drosophila melanogaster

“…It has been shown that the difference in the median lifespan in the dark and light was 24–33%, while in the control wild-type strain the difference in lifespan was 3–17%. Thus, our data point to the important role of the Sir2 gene in the regulation of lifespan under a changing day length (Moskalev and Malysheva, 2010). …”

“…We have suggested that there are two relatively independent genetic mechanisms of the influence of light on lifespan (Moskalev and Malysheva, 2010). The first mechanism is related to the damaging effects of light, leading to a reduced lifespan (Figure 1A), the second mechanism is related to the influence of the dark as a mild stressor, which can stimulate the body's defense system and lead to an increased lifespan (Figure 1B).…”

“…In our studies dSir2 gene mutation led to a significant reduction in lifespan compared with the wild-type strain. The median lifespan of males and females of the strain with a deletion of the Sir2 gene was lower than the median lifespan of the control strain by 19% in males and 33% in females (Moskalev and Malysheva, 2010). The role of sirtuins in the change of lifespan under the influence of different lighting regimes has not previously been studied.…”

“…Moreover, in Drosophila with mutations in genes encoding catalase and Cu, Zn-Sod, a decrease of the Hsp70 induction period is observed with age, which provides evidence of Hsp70 involvement in the response to oxidative stress (Landis et al, 2004). We have suggested that the Hsp70 gene deletions in Drosophila can reduce lifespan in light conditions compared to dark conditions (Moskalev and Malysheva, 2010). Notwithstanding the fact that the median lifespan in the light and in the dark of the strains with Hsp70 gene mutations changed slightly, under the exposure to light, the maximum lifespan dropped significantly (by 7–29%) (Moskalev and Malysheva, 2010).…”

“…We have suggested that the Hsp70 gene deletions in Drosophila can reduce lifespan in light conditions compared to dark conditions (Moskalev and Malysheva, 2010). Notwithstanding the fact that the median lifespan in the light and in the dark of the strains with Hsp70 gene mutations changed slightly, under the exposure to light, the maximum lifespan dropped significantly (by 7–29%) (Moskalev and Malysheva, 2010). …”