The Role of TRPV4 in Regulating Innate Immune Cell Function in Lung Inflammation

Scheraga, Rachel G.; Southern, Brian D.; Grove, L.; Olman, Mitchell A.

doi:10.3389/fimmu.2020.01211

Cited by 32 publications

(22 citation statements)

References 63 publications

(98 reference statements)

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“…Indeed, TRPV2 was documented as being expressed by the cells of both the innate and adaptive immune system, including macrophages, granulocytes, and lymphocytes [ 27 ]. Data regarding TRPV4 expression in immune cells are limited, however, as few studies associated these ion channels with the innate immune system, mostly with macrophages [ 28 , 29 ]. Nevertheless, a few studies showed protein expression of TRPV4 in the human T cells [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Functional Expression of TRPV1 Ion Channel in the Canine Peripheral Blood Mononuclear Cells

Bujak

Kosmala

Majchrzak-Kuligowska

et al. 2021

IJMS

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TRPV1, known as a capsaicin receptor, is the best-described transient receptor potential (TRP) ion channel. Recently, it was shown to be expressed by non-excitable cells such as lymphocytes. However, the data regarding the functional expression of the TRPV1 channel in the immune cells are often contradictory. In the present study, we performed a phylogenetical analysis of the canine TRP ion channels, we assessed the expression of TRPV1 in the canine peripheral blood mononuclear cells (PBMC) by qPCR and Western blot, and we determined the functionality of TRPV1 by whole-cell patch-clamp recordings and calcium assay. We found high expression of TRPV2, -M2, and -M7 in the canine PBMCs, while expression of TRPV1, -V4 and, -M5 was relatively low. We confirmed that TRPV1 is expressed on the protein level in the PBMC and it localizes in the plasma membrane. The whole-cell patch-clamp recording revealed that capsaicin application caused a significant increase in the current density. Similarly, the results from the calcium assay show a dose-dependent increase in intracellular calcium level in the presence of capsaicin that was partially abolished by capsazepine. Our study confirms the expression of TRPV1 ion channel on both mRNA and protein levels in the canine PBMC and indicates that the ion channel is functional.

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Section: Introductionmentioning

confidence: 99%

Functional Expression of TRPV1 Ion Channel in the Canine Peripheral Blood Mononuclear Cells

Bujak

Kosmala

Majchrzak-Kuligowska

et al. 2021

IJMS

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“…Several studies shed the light on the role of different TRP channels in immune and inflammatory responses suggesting that there is interplay between these channels and the production of inflammatory mediators in COVID-19 patients. For instance, it was reported that TRPV4 channel is a regulator for pulmonary inflammation due to its expression in alveolar macrophages [ 35 ]. Particularly, TRPV4 channel is involved in the recruitment of neutrophils and macrophages during lung injury [ 20 ].…”

Section: Resultsmentioning

confidence: 99%

TRP channels in COVID-19 disease: Potential targets for prevention and treatment

Jaffal

Abbas

2021

Chemico-Biological Interactions

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Coronavirus disease 2019 [COVID-19] is a global health threat caused by severe acute respiratory syndrome coronavirus 2 [SARS-CoV2] that requires two proteins for entry: angiotensin-converting enzyme 2 [ACE2] and trans-membrane protease serine 2 [TMPRSS2]. Many patients complain from pneumonia, cough, fever, and gastrointestinal (GI) problems. Notably, different TRP channels are expressed in various tissues infected by SARS-CoV-2. TRP channels are cation channels that show a common architecture with high permeability to calcium [Ca 2+ ] in most sub-families. Literature review shed the light on the possible role of TRP channels in COVID-19 disease. TRP channels may take part in inflammation, pain, fever, anosmia, ageusia, respiratory, cardiovascular, GI and neurological complications related to COVID-19. Also, TRP channels could be the targets for many active compounds that showed effectiveness against SARS-CoV-2. Desensitization or blocking of TRP channels by antibodies, aptamers, small molecules or venoms can be an option for COVID-19 prevention and future treatment. This review provides insights into the involvement of TRP channels in different symptoms and mechanisms of COVID-19, potential treatments targeting these channels and highlights missing gaps in literature.

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“…These data suggest that TRPV4 is activated by deformation of the cytoskeletal backbone rather than direct membrane stretch ( 45 ). Activation of TRPV4, whether direct or indirect, results in Ca 2+ influx, cytoskeletal rearrangement, intracellular signaling, and altered gene expression which influence cellular phenotype and function ( 46 ).…”

Section: Trpv4mentioning

confidence: 99%

“…M. tuberculosis infection results in significant scaring which changes the mechanical properties of the lung. Although TRPV4-dependant control of M. tuberculosis in macrophages has not yet been shown to be tissue-stiffness initiated, macrophage function is influenced by a stiffness-dependent mechanism via TRPV4 ( 46 ). Furthermore, M. tuberculosis also induces TRPV4 protein expression in macrophages, suggesting a complex interaction between TRPV4, M. tuberculosis , and the mechanical properties of the lung matrix ( 47 ).…”

Section: Trpv4mentioning

confidence: 99%

Stretching the Function of Innate Immune Cells

et al. 2021

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The importance of innate immune cells to sense and respond to their physical environment is becoming increasingly recognized. Innate immune cells (e.g. macrophages and neutrophils) are able to receive mechanical signals through several mechanisms. In this review, we discuss the role of mechanosensitive ion channels, such as Piezo1 and transient receptor potential vanilloid 4 (TRPV4), and cell adhesion molecules, such as integrins, selectins, and cadherins in biology and human disease. Furthermore, we explain that these mechanical stimuli activate intracellular signaling pathways, such as MAPK (p38, JNK), YAP/TAZ, EDN1, NF-kB, and HIF-1α, to induce protein conformation changes and modulate gene expression to drive cellular function. Understanding the mechanisms by which immune cells interpret mechanosensitive information presents potential targets to treat human disease. Important areas of future study in this area include autoimmune, allergic, infectious, and malignant conditions.

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The Role of TRPV4 in Regulating Innate Immune Cell Function in Lung Inflammation

Cited by 32 publications

References 63 publications

Functional Expression of TRPV1 Ion Channel in the Canine Peripheral Blood Mononuclear Cells

Functional Expression of TRPV1 Ion Channel in the Canine Peripheral Blood Mononuclear Cells

TRP channels in COVID-19 disease: Potential targets for prevention and treatment

Stretching the Function of Innate Immune Cells

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