The role of type 1 and curli fimbriae of Shiga toxin-producing Escherichia coli in adherence to abiotic surfaces

Cooley, W. A.; Woodward, Martin J.

doi:10.1078/1438-4221-00203

Cited by 149 publications

(133 citation statements)

References 45 publications

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“…coli adhesins mediate binding to alfalfa. Since curli and the Cah protein have been associated with adhesion to inert surfaces in E. coli O157:H7 (11,45) and since these proteins are either absent or not expressed in E. coli K-12 strains (6, 17, 21, 45), we examined whether these structures were able to mediate E. coli K-12 binding to alfalfa sprouts and seed coats. We first determined whether E. coli K-12 strains express curli or the Cah protein under the conditions used to infect alfalfa (incubating samples at room temperature for 2 to 3 days).…”

Section: Resultsmentioning

confidence: 99%

“…coli O157:H7 expresses multiple fimbrial and nonfimbrial adhesins which have no obvious role in pathogenesis and may be involved in adhesion to other surfaces (reviewed in reference 46). For example, curli-expressing thin aggregative fim-briae, which are rarely reported in E. coli O157:H7 compared with other pathogenic E. coli strains (47,48), have been reported to bind eukaryotic extracellular matrix proteins as well as to enhance the formation of E. coli O157:H7 biofilms on inert surfaces (11,38). Biofilm formation may increase E. coli O157:H7 survival and would likely result in protection against many environmental conditions.…”

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confidence: 99%

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Differential Binding of Escherichia coli O157:H7 to Alfalfa, Human Epithelial Cells, and Plastic Is Mediated by a Variety of Surface Structures

Torres

Jeter

Langley

et al. 2005

Appl Environ Microbiol

103

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Escherichia coli O157:H7 carried on plant surfaces, including alfalfa sprouts, has been implicated in food poisoning and outbreaks of disease in the United States. Adhesion to cell surfaces is a key component for bacterial establishment and colonization on many types of surfaces. Several E. coli O157:H7 surface proteins are thought to be important for adhesion and/or biofilm formation. Therefore, we examined whether mutations in several genes encoding potential adhesins and regulators of adherence have an effect on bacterial binding to plants and also examined the role of these genes during adhesion to Caco-2 cells and during biofilm formation on plastic in vitro. The genes tested included those encoding adhesins (cah, aidA1, and ompA) and mediators of hyperadherence (tdcA, yidE, waaI, and cadA) and those associated with fimbria formation (csgA, csgD, and lpfD2). The introduction of some of these genes (cah, aidA1, and csg loci) into an E. coli K-12 strain markedly increased its ability to bind to alfalfa sprouts and seed coats. The addition of more than one of these genes did not show an additive effect. In contrast, deletion of one or more of these genes in a strain of E. coli O157:H7 did not affect its ability to bind to alfalfa. Only the absence of the ompA gene had a significant effect on binding, and the plant-bacterium interaction was markedly reduced in a tdcA ompA double mutant. In contrast, the E. coli O157:H7 ompA and tdcA ompA mutant strains were only slightly affected in adhesion to Caco-2 cells and during biofilm formation. These findings suggest that some adhesins alone are sufficient to promote binding to alfalfa and that they may exist in E. coli O157:H7 as redundant systems, allowing it to compensate for the loss of one or more of these systems. Binding to the three types of surfaces appeared to be mediated by overlapping but distinct sets of genes. The only gene which appeared to be irreplaceable for binding to plant surfaces was ompA.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Differential Binding of Escherichia coli O157:H7 to Alfalfa, Human Epithelial Cells, and Plastic Is Mediated by a Variety of Surface Structures

Torres

Jeter

Langley

et al. 2005

Appl Environ Microbiol

103

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“…In contrast, enteroinvasive E. coli (EIEC), Shigella spp. and enterohaemorrhagic E. coli (EHEC) regularly do not express curli fimbriae in vitro (Bian et al, 2000;Cookson et al, 2002;Patri et al, 2000;Sakellaris et al, 2000;Sjöbring et al, 1994;Uhlich et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Expression of cellulose and curli fimbriae by Escherichia coli isolated from the gastrointestinal tract

Bokranz

Xiao-da

Tschäpe

et al. 2005

Journal of Medical Microbiology

228

235

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Escherichia coli colonizes the gastrointestinal tract of humans; however, little is known about the features of commensal strains. This study investigated whether expression of the biofilm extracellular matrix components cellulose and curli fimbriae is found among commensal isolates. Fifty-two E. coli strains were isolated from faecal samples and, as a control, 24 strains from urinary tract infections were also used. Faecal isolates were characterized by serotyping and phylogenetically grouped by PCR. The genotype was determined by PFGE and the presence of virulence factors was assessed. Co-expression of cellulose and curli fimbriae at 28 8C and 37 8C was typical for faecal isolates, while urinary tract infection strains typically expressed the extracellular matrix components at 28 8C only. Knockout studies in a representative faecal isolate revealed that the response regulator CsgD regulated cellulose and curli fimbriae, as found previously in Salmonella enterica. In contrast to S. enterica, at 37 8C pellicle formation occurred in the absence of cellulose and curli fimbriae. The gastrointestinal tract represents a source of biofilm-forming bacteria, which can spread to susceptible sites.

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“…In addition to antigen 43, fimbriae and fimbria-like adhesins are important in biofilm formation in E. coli and other organisms (5,6,12,14,20,33,49,55,59). These large surface structures also play a key role in attachment to epithelial cells and pathogenesis (11,31,40).…”

mentioning

confidence: 99%

ASerratia marcescensOxyR Homolog Mediates Surface Attachment and Biofilm Formation

Shanks

Stella²,

Kalivoda³

et al. 2007

J Bacteriol

104

131

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OxyR is a conserved bacterial transcription factor with a regulatory role in oxidative stress response. From a genetic screen for genes that modulate biofilm formation in the opportunistic pathogen Serratia marcescens, mutations in an oxyR homolog and predicted fimbria structural genes were identified. S. marcescens oxyR mutants were severely impaired in biofilm formation, in contrast to the hyperbiofilm phenotype exhibited by oxyR mutants of Escherichia coli and Burkholderia pseudomallei. Further analysis revealed that OxyR plays a role in the primary attachment of cells to a surface. Similar to what is observed in other bacterial species, S. marcescens OxyR is required for oxidative stress resistance. Mutations in oxyR and type I fimbrial genes resulted in severe defects in fimbria-associated phenotypes, revealing roles in cell-cell and cell-biotic surface interactions. Transmission electron microscopy revealed the absence of fimbria-like surface structures on an OxyR-deficient strain and an enhanced fimbrial phenotype in strains bearing oxyR on a multicopy plasmid. The hyperfimbriated phenotype conferred by the multicopy oxyR plasmid was absent in a type I fimbrial mutant background. Real-time reverse transcriptase PCR indicated an absence of transcripts from a fimbrial operon in an oxyR mutant that were present in the wild type and a complemented oxyR mutant strain. Lastly, chromosomal P lac -mediated expression of fimABCD was sufficient to restore wild-type levels of yeast agglutination and biofilm formation to an oxyR mutant. Together, these data support a model in which OxyR contributes to early stages of S. marcescens biofilm formation by influencing fimbrial gene expression.

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The role of type 1 and curli fimbriae of Shiga toxin-producing Escherichia coli in adherence to abiotic surfaces

Cited by 149 publications

References 45 publications

Differential Binding of Escherichia coli O157:H7 to Alfalfa, Human Epithelial Cells, and Plastic Is Mediated by a Variety of Surface Structures

Differential Binding of Escherichia coli O157:H7 to Alfalfa, Human Epithelial Cells, and Plastic Is Mediated by a Variety of Surface Structures

Expression of cellulose and curli fimbriae by Escherichia coli isolated from the gastrointestinal tract

ASerratia marcescensOxyR Homolog Mediates Surface Attachment and Biofilm Formation

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