The role of UDP-glycosyltransferases in xenobioticresistance

Dimunová, Diana; Matoušková, Petra; Podlipná, Radka; Boušová, Iva; Skálová, Lenka

doi:10.1080/03602532.2022.2083632

Cited by 25 publications

(13 citation statements)

References 150 publications

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“…[46][47][48] UGTs are common, highly diverse enzymes that mediate the metabolization of xenobiotics in insects, including pyrethroids and neonicotinoids. [49][50][51][52] The specific UDP-glucosyltransferase overexpressed in our study was highly similar to the 2- Galactosylceramides are cerebrosides commonly associated with vertebrates, while ceramide phosphoethanolamine is the cerebroside reported as the main constituent of myelinic membranes in invertebrates. 53,54 Additionally, the domain families of 2-hydroxyacylsphingosine 1-beta-galactosyltransferases from hemipterans differ from those of human 2-hydroxyacylsphingosine 1-beta-galactosyltransferases (data not shown), supporting the different role of this UGT in insects.…”

Section: Discussionsupporting

confidence: 64%

“…Increased activity of GSTs is commonly reported as a resistance mechanism to pesticides in insects 46–48 . UGTs are common, highly diverse enzymes that mediate the metabolization of xenobiotics in insects, including pyrethroids and neonicotinoids 49–52 . The specific UDP‐glucosyltransferase overexpressed in our study was highly similar to the 2‐hydroxyacylsphingosine 1‐beta‐galactosyltransferase‐like gene from Halyomorpha halys.…”

Section: Discussionmentioning

confidence: 51%

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Transcriptomic investigation of the molecular mechanisms underlying resistance to the neonicotinoid thiamethoxam and the pyrethroid lambda‐cyhalothrin in Euschistus heros (Hemiptera: Pentatomidae)

Lira,

Nascimento,

Bass

et al. 2023

Pest Management Science

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BACKGROUNDLaboratory‐selected resistant strains of Euschistus heros to thiamethoxam (NEO) and lambda‐cyhalothrin (PYR) were recently reported in Brazil. However, the mechanisms conferring resistance to these insecticides in E. heros remain unresolved. We utilized comparative transcriptome profiling and SNP calling of susceptible and resistant strains of E. heros to investigate the molecular mechanism(s) underlying resistance.RESULTSThe E. heros transcriptome was assembled, generating 91,673 transcripts with a mean length of 720 bp and N50 of 1795 bp. Comparative gene expression analysis between the susceptible (SUS) and NEO strains identified 215 significantly differentially expressed (DE) transcripts. DE transcripts associated with the xenobiotic metabolism were all up‐regulated in the NEO strain. The comparative analysis of the SUS and PYR strains identified 204 DE transcripts, including an esterase (esterase FE4), a glutathione‐S‐transferase, an ABC transporter (ABCC1), and aquaporins that were up‐regulated in the PYR strain. We identified 9,588 and 15,043 non‐synonymous SNPs in the PYR and NEO strains. One of the SNPs (D70N) detected in the NEO strain occurs in a subunit (α5) of the nAChRs, the target‐site of neonicotinoid insecticides. Nevertheless, this residue position in α5 is not conserved among insects.CONCLUSIONSNeonicotinoid and pyrethroid resistance in laboratory‐selected E. heros is associated with a potential metabolic resistance mechanism by the overexpression of proteins commonly involved in the three phases of xenobiotic metabolism. Together these findings provide insight into the potential basis of resistance in E. heros and will inform the development and implementation of resistance management strategies against this important pest.This article is protected by copyright. All rights reserved.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 51%

Transcriptomic investigation of the molecular mechanisms underlying resistance to the neonicotinoid thiamethoxam and the pyrethroid lambda‐cyhalothrin in Euschistus heros (Hemiptera: Pentatomidae)

Lira,

Nascimento,

Bass

et al. 2023

Pest Management Science

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“…UGT enzymes catalyse the conjugation of sugars moieties to a variety of acceptor molecules, including herbicides which can lead to detoxification (84). High sequence diversity among UGTs genes leads to protein structural heterogeneity, which in turn facilitates a wide range of acceptor molecules, including herbicides (25).…”

Section: Discussionmentioning

confidence: 99%

“…Uridine diphosphate (UDP) glycosyltransferases (UGTs) are part of the largest family (Family 1) of the glycosyltransferase superfamily, and this subset of UGTs have been widely associated with herbicide resistance (22,23). UGTs are present in bacteria, fungi, plants, and animals and catalyse the transfer of glycosyl residues from activated nucleotide sugars, such as uridine diphosphate glucose, to acceptor molecules (24).…”

Section: Introductionmentioning

confidence: 99%

ATP binding cassette transporters and uridine diphosphate glycosyltransferases are ancient protein families that evolved roles in herbicide resistance through exaptation

Caygill¹,

Dolan²

2023

Preprint

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ATP-binding cassette (ABC) transporters actively transport various substances across membranes, while uridine diphosphate (UDP) glycosyltransferases (UGTs) are proteins that catalyse the chemical modification of various organic compounds. Both of these protein superfamilies have been associated with conferring herbicide resistance in weeds. Little is known about the evolutionary history of these protein families in the Archaeplastida. To infer the evolutionary histories of these protein superfamilies, we compared protein sequences collected from 10 species which represent distinct lineages of the Archaeplastida – the lineage including glaucophyte algae, rhodophyte algae, chlorophyte algae and the streptophytes – and generated phylogenetic trees. We show that ABC transporters were present in the last common ancestor of the Archaeplastida which lived 1.6 billion years ago, and the major clades identified in extant plants were already present then. Conversely, we only identified UGTs in members of the streptophyte lineage, which suggests a loss of these proteins in earlier diverging Archaeplastida lineages or arrival of UGTs into a common ancestor of the streptophyte lineage through horizontal gene transfer from a non-Archaeplastida eukaryote lineage. We found that within the streptophyte lineage, most diversification of the UGT protein family occurred in the vascular lineage, with 17 of the 20 clades identified in extant plants present only in vascular plants. Based on our findings, we conclude that ABC transporters and UGTs are ancient protein families which diversified during Archaeplastida evolution, which may have evolved to for developmental functions as plants began to occupy new environmental niches and are now being selected to confer resistance to a diverse range of herbicides in weeds.

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“…These toxins facilitate fungal invasion or act as immunosuppressive compounds [ 24 ], accelerating insect death and tissue decomposition, and thereby promoting reproduction of the pathogens themselves [ 25 ]. In insects, a variety of enzyme families contribute to the metabolic degradation of harmful foreign substances; these include cytochrome P450 enzymes (P450s) [ 26 , 27 ], glutathione-S-transferases (GSTs) [ 28 ], the carboxylate enzyme system (CarbEs) [ 29 ], the UDP-glucosyltransferase system [ 30 ], and ATP-binding cassette transporters (ABCs) [ 31 ]. The overexpression (or mutation) of various detoxification-enzyme genes can cause changes in the sensitivity of insects to various types of insecticides.…”

Section: Introductionmentioning

confidence: 99%

Preliminary Analysis of Transcriptome Response of Dioryctria sylvestrella (Lepidoptera: Pyralidae) Larvae Infected with Beauveria bassiana under Short-Term Starvation

Guo

Jia

Chen

et al. 2023

Insects

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The Dioryctria genus contains several destructive borer pests that are found in coniferous forests in the Northern Hemisphere. Beauveria bassiana spore powder was tested as a new method of pest control. In this study, Dioryctria sylvestrella (Lepidoptera: Pyralidae) was used as the object. A transcriptome analysis was performed on a freshly caught group, a fasting treatment control group, and a treatment group inoculated with a wild B. bassiana strain, SBM-03. Under the conditions of 72-h fasting and a low temperature of 16 ± 1 °C, (i) in the control group, 13,135 of 16,969 genes were downregulated. However, in the treatment group, 14,558 of 16,665 genes were upregulated. (ii) In the control group, the expression of most genes in the upstream and midstream of the Toll and IMD pathways was downregulated, but 13 of the 21 antimicrobial peptides were still upregulated. In the treatment group, the gene expression of almost all antimicrobial peptides was increased. Several AMPs, including cecropin, gloverin, and gallerimycin, may have a specific inhibitory effect on B. bassiana. (iii) In the treatment group, one gene in the glutathione S-transferase system and four genes in the cytochrome P450 enzyme family were upregulated, with a sharp rise in those that were upregulated significantly. In addition, most genes of the peroxidase and catalase families, but none of the superoxide dismutase family were upregulated significantly. Through innovative fasting and lower temperature control, we have a certain understanding of the specific defense mechanism by which D. sylvestrella larvae may resist B. bassiana in the pre-wintering period. This study paves the way for improving the toxicity of B. bassiana to Dioryctria spp.

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The role of UDP-glycosyltransferases in xenobioticresistance

Cited by 25 publications

References 150 publications

Transcriptomic investigation of the molecular mechanisms underlying resistance to the neonicotinoid thiamethoxam and the pyrethroid lambda‐cyhalothrin in Euschistus heros (Hemiptera: Pentatomidae)

Transcriptomic investigation of the molecular mechanisms underlying resistance to the neonicotinoid thiamethoxam and the pyrethroid lambda‐cyhalothrin in Euschistus heros (Hemiptera: Pentatomidae)

ATP binding cassette transporters and uridine diphosphate glycosyltransferases are ancient protein families that evolved roles in herbicide resistance through exaptation

Preliminary Analysis of Transcriptome Response of Dioryctria sylvestrella (Lepidoptera: Pyralidae) Larvae Infected with Beauveria bassiana under Short-Term Starvation

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