The Role of VAMP7/TI‐VAMP in Cell Polarity and Lysosomal Exocytosis <i>in vivo</i>

Sato, Mahito; Yoshimura, Sho; Hirai, Rika; Goto, Ayako; Kunii, Masataka; Atik, Nur; Satō, Takashi; Sato, Ken; Harada, Reiko; Shimada, Junko; Hatabu, Toshimitsu; Yorifuji, Hiroshi; Harada, Akira

doi:10.1111/j.1600-0854.2011.01247.x

Cited by 31 publications

(36 citation statements)

References 27 publications

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“…Previous experiments in cultured neurons used silencing approaches and expression of the Longin as dominant-negative fragment to conclude that TI-VAMP was involved in neurite growth. Based on the present data and Sato et al (2011), it is likely that the abovementionedcompensatorymechanismsoccurpreferentiallywhenTI-VAMP is deleted early in development. We conclude that TI-VAMP functions during development in vivo are either dispensable or compensated for by other SNAREs allowing the KO mice to fulfill major developmental processes.…”

Section: Discussionmentioning

confidence: 97%

“…We did not find any defect in growth of TI-VAMP KO cultured neurons in presence or absence of feeding astrocytes. Using hippocampal neurons from a different TI-VAMP KO mouse, Sato et al (2011) reported a modest effect on axonal growth of neurons grown for 3 DIV. Therefore, we are led to the idea that one or several other v-SNAREs or other mechanisms compensate for TI-VAMP functions.…”

Section: Discussionmentioning

confidence: 99%

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Absence of TI-VAMP/Vamp7 Leads to Increased Anxiety in Mice

Danglot¹,

Zylbersztejn²,

Petković³

et al. 2012

J. Neurosci.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Absence of TI-VAMP/Vamp7 Leads to Increased Anxiety in Mice

Danglot¹,

Zylbersztejn²,

Petković³

et al. 2012

J. Neurosci.

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“…In conclusion, VAMP7 KO MEFs show phenotypes clearly related to the traffic of lipids and proteins known to reside in membrane domains. While these phenotypic traits do not preclude cell and organism life, 55,56 they may nevertheless induce profound signaling defects 20 which largely remain to explore. Further studies are required to address the impact of this mechanism in the organism and also how a v-SNARE which primarily mediates membrane fusion may also be required at earlier stages of membrane traffic like sorting of some components of membrane domains.…”

Section: Discussionmentioning

confidence: 99%

Role of tetanus neurotoxin insensitive vesicle-associated membrane protein in membrane domains transport and homeostasis

et al. 2015

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Keywords: exocytosis, Golgi apparatus, SNARE, sphingolipids, TI-VAMP/VAMP7 Abbreviations: BFA, Brefeldin A; Cer, Ceramide; ER, Endoplasmic Reticulum; GlcCer, Glucosylceramide; GM3, ganglioside monosialic acid 3; GPI, Glycosylphosphatidylinositol; GSL, Glycosphingolipids; LC, Long Chain; PI, Phosphatidylinositide; PM, Plasma Membrane; SM, Sphingomyelin; TGN, D Trans-Golgi Network; TI-VAMP/VAMP7, Tetanus neurotoxin-insensitive vesicle-associated membrane protein / Vesicle associated membrane protein 7; VLC, very long vhain; VSVG, Vesicular Stomatitis Virus GlycoproteinBiological membranes in eukaryotes contain a large variety of proteins and lipids often distributed in domains in plasma membrane and endomembranes. Molecular mechanisms responsible for the transport and the organization of these membrane domains along the secretory pathway still remain elusive. Here we show that vesicular SNARE TI-VAMP/VAMP7 plays a major role in membrane domains composition and transport. We found that the transport of exogenous and endogenous GPI-anchored proteins was altered in fibroblasts isolated from VAMP7-knockout mice. Furthermore, disassembly and reformation of the Golgi apparatus induced by Brefeldin A treatment and washout were impaired in VAMP7-depleted cells, suggesting that loss of VAMP7 expression alters biochemical properties and dynamics of the Golgi apparatus. In addition, lipid profiles from these knockout cells indicated a defect in glycosphingolipids homeostasis. We conclude that VAMP7 is required for effective transport of GPI-anchored proteins to cell surface and that VAMP7-dependent transport contributes to both sphingolipids and Golgi homeostasis.

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“…Samples were fixed using perfusion with 4% (w/v) PFA in 0.1 M phosphate buffer (pH 7.4) and processed as previously described (Harada et al, 1990, Sato et al, 2011. We performed hematoxylin-eosin staining using standard histological procedures.…”

Section: Histology and Western Blot Analysesmentioning

confidence: 99%

“…We performed hematoxylin-eosin staining using standard histological procedures. Western blot analyses were performed as previously described (Sato et al, 2011) and 10 mg of protein was loaded per lane.…”

Section: Histology and Western Blot Analysesmentioning

confidence: 99%

Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis

Satō¹,

Iwano²,

Kunii³

et al. 2014

Development

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The small GTP-binding protein Rab8 is known to play an essential role in intracellular transport and cilia formation. We have previously demonstrated that Rab8a is required for localising apical markers in various organisms. Rab8a has a closely related isoform, Rab8b. To determine whether Rab8b can compensate for Rab8a, we generated Rab8b-knockout mice. Although the Rab8b-knockout mice did not display an overt phenotype, Rab8a and Rab8b double-knockout mice exhibited mislocalisation of apical markers and died earlier than Rab8a-knockout mice. The apical markers accumulated in three intracellular patterns in the double-knockout mice. However, the localisation of basolateral and/or dendritic markers of the double-knockout mice seemed normal. The morphology and the length of various primary and/or motile cilia, and the frequency of ciliated cells appeared to be identical in control and double-knockout mice. However, an additional knockdown of Rab10 in double-knockout cells greatly reduced the percentage of ciliated cells. Our results highlight the compensatory effect of Rab8a and Rab8b in apical transport, and the complexity of the apical transport process. In addition, neither Rab8a nor Rab8b are required for basolateral and/or dendritic transport. However, simultaneous loss of Rab8a and Rab8b has little effect on ciliogenesis, whereas additional loss of Rab10 greatly affects ciliogenesis.

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The Role of VAMP7/TI‐VAMP in Cell Polarity and Lysosomal Exocytosis in vivo

Cited by 31 publications

References 27 publications

Absence of TI-VAMP/Vamp7 Leads to Increased Anxiety in Mice

Absence of TI-VAMP/Vamp7 Leads to Increased Anxiety in Mice

Role of tetanus neurotoxin insensitive vesicle-associated membrane protein in membrane domains transport and homeostasis

Rab8a and Rab8b are essential for several apical transport pathways but insufficient for ciliogenesis

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