The Role of Virulence Factors in the Pathogenicity of Colletotrichum sp.

Villa-Rivera, Maria Guadalupe; Conejo-Saucedo, Ulises; Lara-Márquez, Alicia; Cano-Camacho, Horacio; López-Romero, Everardo; Zavala-Páramo, María Guadalupe

doi:10.2174/1389203717666160813160727

Cited by 16 publications

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“…Its global occurrence causes significant losses in tropical and subtropical regions [39]. Although the genus contains species with different lifestyles, plants can be infected during any stage of development and symptoms appear after colonization of fungi, which is characterized by necrotic lesions in leaves [40]. Therefore, due to the lack of information about its occurrence and aggressiveness, there is a need to determine the susceptibility of plant species to validate the prospection of mycoherbicides, adopting the pathogenicity test proposed by Wapshere [23], widely used in biological control programs.…”

Section: Resultsmentioning

confidence: 99%

“…Isolates of the collected samples were made and the fragments containing the fungal structures were grown in Petri plates containing Potato Carrot Agar (PCA) culture medium. The isolates were incubated at 25 ± 3 °C in the dark for eight days [19]. The identification of organisms at the genus level was carried out using the identification key of Barnett and Hunter [20], by observations of the structures under a stereoscopic microscope.…”

Section: Methodsmentioning

confidence: 99%

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Biological Potencial of Colletotrichum typhae H.C Greene mycoherbicide for Typha domingensis Pers

Maia

Melo

Barreto

et al. 2018

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24 The anthropic interference in aquatic ecosystems, favors the disordered colonization of T. domingensis, 25 damaging the production of hydroelectric power and river traffic. Thus, the objective of this study was 26 to evaluate the potential of C. typhae as a mycoherbicide in the control of T. domingensis, in vitro and 27 in greenhouse. 107 samples of symptomatic T. domingensis leaves were collected in flooded areas of 28 rivers in Brazil, with identification and isolation of the collected fungal species. The concentration of 29 inoculum was determined to evaluate the incidence and severity of the disease, the influence of 30 temperature on mycelial growth and conidia germination, the effect of temperature and leaf wetness 31 period on T. domingensis infection by C. typhae and the host range test. The growth of the colonies of 32 C. typhae was higher at 25 to 30 ºC, there was no interference of the photoperiod on germination of the 33 spores, but the highest percentage of germination occurred at 17.39 ºC. The influence of environmental 2 34 conditions on infection of inoculated leaves of T. dominguensis indicated that at 15 ºC and the period of 35 leaf wetness of 48 hours promoted the highest incidence of the disease, as well as the severity for the 36 same period of leaf wetness. The specificity test showed that C. typhae is specific and pathogenic to T. 37 domingensis. Being this the first report of the occurrence of this pathogen in aquatic macrophytes of 38 this species and in T. domingensis in Brazil. 39 Keywords: Biological control; Aquatic macrophytes, Leaf wetness 40 41 Introduction 42 Typha domingensis Pers. is an invasive macrophyte found in the Americas, Europe, Africa, Asia and 43 Oceania, being considered as a native species of South America, occurring throughout Brazil [1]. It is 44 propagated either by seeds or vegetatively, by rhizomes, with vigorous growth by the decomposition 45 and assimilation of organic matter as a source of nutrients, reaching about seven tons of rhizomes per 46 hectare [2]. Thus, T. domingensis is used as a biological filter for urban sewage, industrial effluents rich 47 in heavy metals and erosion control in drainage channels and reservoir banks [3]. 48However, anthropic interference in aquatic ecosystems favors the colonization of T. domingensis, 49 which may hinder the production of hydroelectric power, river traffic and agricultural irrigation [4]. In 50 the United States, this macrophyte accounts for the degradation of almost 12.000 ha -1 of Florida 51 marshes, due its aggressive growth in response to eutrophication by nitrates and phosphates from 52 agricultural and urban waste and frequent fires [5]. In Brazil, it is estimated that the intense growth of 53 T. domingensis in reservoirs of the country's hydroelectric dams extends to about 300 ha -1 in the water 54 mirror. These changes contribute to the reduction of water quality and biodiversity patterns in 55 environments colonized by this species [6]. 56Up to a certain limit, the development of aquatic vegetation c...

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Biological Potencial of Colletotrichum typhae H.C Greene mycoherbicide for Typha domingensis Pers

Maia

Melo

Barreto

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…In addition, several DEGs associated with appressorium development were significantly upregulated at the early stages or downregulated at the end of the infection. Within our DEG list, a gene encoding a putative cAMP dependent protein kinase was identified with 88.22% similarity to a cAMP dependant candidate gene of C. higginsianum involved in appressorium production (e-value = 0) [ 11 , 19 ]. This gene was upregulated from 24 hpi to 60 hpi.…”

Section: Discussionmentioning

confidence: 99%

“…Effectors such as CgDN3 of C. gloeosporioides [ 16 ], ChEC36 and ChEC6 of C. higginsianum [ 14 ] were found to be expressed in the biotrophic stage, including in unpenetrated appressoria in planta, notably for the two latter [ 14 , 17 ]. Among the Colletotrichum studied so far, the biotrophic phase is also characterized by a higher expression of genes associated with secondary metabolism and genes encoding carbohydrate active enzymes (CAZymes) [ 11 , 12 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Deciphering the Infectious Process of Colletotrichum lupini in Lupin through Transcriptomic and Proteomic Analysis

et al. 2020

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The fungal phytopathogen Colletotrichum lupini is responsible for lupin anthracnose, resulting in significant yield losses worldwide. The molecular mechanisms underlying this infectious process are yet to be elucidated. This study proposes to evaluate C. lupini gene expression and protein synthesis during lupin infection, using, respectively, an RNAseq-based transcriptomic approach and a mass spectrometry-based proteomic approach. Patterns of differentially-expressed genes in planta were evaluated from 24 to 84 hours post-inoculation, and compared to in vitro cultures. A total of 897 differentially-expressed genes were identified from C. lupini during interaction with white lupin, of which 520 genes were predicted to have a putative function, including carbohydrate active enzyme, effector, protease or transporter-encoding genes, commonly described as pathogenicity factors for other Colletotrichum species during plant infection, and 377 hypothetical proteins. Simultaneously, a total of 304 proteins produced during the interaction were identified and quantified by mass spectrometry. Taken together, the results highlight that the dynamics of symptoms, gene expression and protein synthesis shared similarities to those of hemibiotrophic pathogens. In addition, a few genes with unknown or poorly-described functions were found to be specifically associated with the early or late stages of infection, suggesting that they may be of importance for pathogenicity. This study, conducted for the first time on a species belonging to the Colletotrichum acutatum species complex, presents an opportunity to deepen functional analyses of the genes involved in the pathogenicity of Colletotrichum spp. during the onset of plant infection.

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“…During disease development, Colletotrichum alkalinizes host-tissue by the active secretion of ammonia [11]. The alkalinization of host tissue stimulates appressorium formation [12], and the secretion of pathogenicity factors, such as pectate lyase [13], cutinase, and peptidases [14]. In the necrotrophic phase berries rot and develop the classical disease symptoms that include circular, reddish brown spots on skin, which enlarge to include entire berry.…”

Section: Introductionmentioning

confidence: 99%

Colletotrichumspecies associated to ripe rot disease of grapes in the “Serra Gaucha” region of Southern Brazil

et al. 2019

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Ripe rot disease caused by Colletotrichum (Glomerella) has become a serious problem for viticulture in Southern Brazil. Global warming contributes to the increase of this devastating and difficult to control disease. Several species of Colletotrichum, with different phytopathological characteristics, have been associated with ripe rot disease in different viticultural regions. In this article, a total of 63 fungi were isolated from grapes showing symptoms of ripe rot disease, and classified by sequencing of ITS region, and compared with the sequences deposited in GenBank. The isolates were included in three clades of Colletotrichum: 84.1% belonged to the “gloeosporioides” clade, 3.2% to the “boninense” clade, and 12.7% to the “acutatum” clade. Of the 53 isolates included in the “gloeosporioides” clade, 44.4% were classified as C. viniferum/C. ampelinum, 37.1% as C. fruticola, 13.0% as C. kahawae and 5.5% as a species related to C. fruticola. In turn, the two isolates of the “boninense” clade were classified as C. kartii/C. phyllanthi, and the six “acutatum” isolates were similar to C. acutatum and C. nymphaeae reference materials. The identified species were previously linked to ripe rot disease in other viticulture regions of the world, but the frequency of some species in southern Brazil is particularly different.

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The Role of Virulence Factors in the Pathogenicity of Colletotrichum sp.

Cited by 16 publications

References 0 publications

Biological Potencial of Colletotrichum typhae H.C Greene mycoherbicide for Typha domingensis Pers

Biological Potencial of Colletotrichum typhae H.C Greene mycoherbicide for Typha domingensis Pers

Deciphering the Infectious Process of Colletotrichum lupini in Lupin through Transcriptomic and Proteomic Analysis

Colletotrichumspecies associated to ripe rot disease of grapes in the “Serra Gaucha” region of Southern Brazil

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