It is well understood that significant point sources of microplastics (MPs) to aquatic systems originate from urban areas. However, measurements of MP levels in water and organisms from urban and nonurban areas do not always reflect the expected effect of urban point source contamination. Despite the potential for different types of MPs originating from urban and nonurban areas, limited information regarding the size distribution of particles in different ecosystem compartments is provided. We found significant MP contamination in the water of an agriculturally dominated, small, cold-water stream. We found additional effects of urbanization on MP contamination in both water and organisms in stream reaches within and downstream of an urban area. Additionally, we found significant differences in the dominant size of MPs among water, macroinvertebrates, and trout.
AbstractWe examined the influence of point and nonpoint source contamination on microplastic (MP) levels in water, macroinvertebrates, and trout in a small, cold-water stream in western Wisconsin, U.S.A. We collected samples along an urbanization gradient centered around a 6-mile corridor that receives numerous MP inputs from stormwater outfalls and a wastewater plant. We digested samples using a wet peroxide oxidation method. Particles filtered from digestates were stained with Nile Red dye, then viewed using fluorescence microscopy. We quantified fluorescing MPs using ImageJ software. MP concentrations in water increased significantly from upstream to downstream reaches and appeared to influence organism MP levels. Macroinvertebrates and trout collected upstream of the city had significantly lower MP levels than macroinvertebrates and trout collected within the city, and for trout, downstream of the city. MP particle lengths were significantly longer in