The role of Zinc Binding Protein‐89 (ZBP‐89) in basal and IL‐1 induced expression of matrix metalloproteinase‐3 (MMP3)

Abstract

MMP‐3 plays an important role in normal and pathological tissue remodeling, and its expression of is regulated by a variety of cytokines, growth factors, and hormones, mainly at the level of transcription. Transcription factor ZBP‐89 has been shown to interact with a polymorphic promoter sequence and activate transcription of the MMP‐3 gene when over‐expressed. To examine the role of ZBP‐89 in basal and IL‐1‐induced expression of MMP3 we knocked down ZBP‐89 in the MG63 cell line. MMP‐3 mRNA expression was measured by real‐time PCR and compared with expression in a negative control cell line. The basal expression of MMP‐3 was significantly lower in the knockdown cell line when compared to negative control. This is consistent with ZBP‐89 as an activator of basal expression. IL‐1 fold induction was significantly higher at 6 hours in the knockdown cell line, which is consistent with a role of ZBP‐89 as a repressor of IL‐1 induced expression of MMP‐3.