2017
DOI: 10.1093/glycob/cwx080
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The role of Zn2+, dimerization and N-glycosylation in the interaction of Auxin-Binding Protein 1 (ABP1) with different auxins

Abstract: Auxin is critical for plant growth and development. The main natural auxin is indole-3-acetic acid (IAA), whereas 1-naphthalene acetic acid (NAA) is a synthetic form. Auxin-Binding Protein 1 (ABP1) specifically binds auxins, presumably playing roles as receptor in nontranscriptional cell responses. ABP1 structure was previously established from maize at 1.9 Å resolution. To gain further insight on ABP1 structural biology, this study was carried out employing molecular dynamics simulations of the complete model… Show more

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Cited by 6 publications
(2 citation statements)
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“…Moreover, other members of the TIR1/AFBfamily of F-box proteins might also be involved in the adventitious rooting process. Molecular dynamics studies have shown that the interaction of NAA and ABP1 of Arabidopsis and maize is more stable than that of IAA with the same receptor (Da Costa et al, 2017), which might contribute to some of the relatively stronger induction of auxin-related gene expression and phenotypic responses by the former auxin.…”
Section: Discussionmentioning
confidence: 99%
“…Moreover, other members of the TIR1/AFBfamily of F-box proteins might also be involved in the adventitious rooting process. Molecular dynamics studies have shown that the interaction of NAA and ABP1 of Arabidopsis and maize is more stable than that of IAA with the same receptor (Da Costa et al, 2017), which might contribute to some of the relatively stronger induction of auxin-related gene expression and phenotypic responses by the former auxin.…”
Section: Discussionmentioning
confidence: 99%
“…His-tagged ABP1 and ABL1, expressed in and purified from HEK293T cells, bound NAA with a K D of 11.9 μM and 17.1 μM, respectively, whereas His-tagged ABP1-5 and ABL1-M2 failed to bind NAA (Figure S5C). Compared with Arabidopsis protoplast-expressed proteins, the lower auxin binding affinity for HEK293T cell-expressed ABL1 and ABP1 may result from their possible incorrect folding and/or lack of proper modifications such as glycosylation 41,43 in HEK293T cells, and/or from some factors co-purified from Arabidopsis protoplasts.…”
Section: Resultsmentioning
confidence: 99%