The Role of β, γ-Subunits of Guanine Nucleotide Binding Proteins in Control of a Reconstituted Signal Transmission Chain Containing Purified Components of the Adenylate Cyclase System

Im, Mie‐Jae; Holzhöfer, Andreas; Keenan, A. K.; Gierschik, Peter; Hekman, Mirko; Helmreich, Ernst; Pfeuffer, Thomas

doi:10.3109/10799898709054977

Cited by 14 publications

(2 citation statements)

References 43 publications

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“…In many systems, receptor regulation of inositol lipid hydrolysis is sensitive to pertussis toxin (216). The observation that G protein ␤␥-subunits activate the PLC-␤ enzymes both in vitro and in transient transfection assays provides a biochemical explanation for this finding (54,55,78,137,247). ␤␥-Subunits can activate all of the PLC-␤ isoenzymes.…”

Section: Inositol Lipid-specific Plcmentioning

confidence: 91%

Physiological Regulation of G Protein-Linked Signaling

Morris

Malbon

1999

Physiological Reviews

403

294

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Heterotrimeric G proteins in vertebrates constitute a family molecular switches that transduce the activation of a populous group of cell-surface receptors to a group of diverse effector units. The receptors include the photopigments such as rhodopsin and prominent families such as the adrenergic, muscarinic acetylcholine, and chemokine receptors involved in regulating a broad spectrum of responses in humans. Signals from receptors are sensed by heterotrimeric G proteins and transduced to effectors such as adenylyl cyclases, phospholipases, and various ion channels. Physiological regulation of G protein-linked receptors allows for integration of signals that directly or indirectly effect the signaling from receptor-->G protein-->effector(s). Steroid hormones can regulate signaling via transcriptional control of the activities of the genes encoding members of G protein-linked pathways. Posttranscriptional mechanisms are under physiological control, altering the stability of preexisting mRNA and affording an additional level for regulation. Protein phosphorylation, protein prenylation, and proteolysis constitute major posttranslational mechanisms employed in the physiological regulation of G protein-linked signaling. Drawing upon mechanisms at all three levels, physiological regulation permits integration of demands placed on G protein-linked signaling.

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Section: Inositol Lipid-specific Plcmentioning

confidence: 91%

Physiological Regulation of G Protein-Linked Signaling

Morris

Malbon

1999

Physiological Reviews

403

294

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“…the receptor (Florio & Sternweis, 1985; Im et al, 1987; . Upon binding of agonist, the receptor becomes activated and undergoes a conformational change that is transmitted to the a subunit, causing the affinity of the a subunit for GDP to decrease.…”

mentioning

confidence: 99%

G proteins: Critical control points for transmembrane signals

Neer

1994

Protein Science

121

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Heterotrimeric GTP-binding proteins (G proteins) that are made up of alpha and beta gamma subunits couple many kinds of cell-surface receptors to intracellular effector enzymes or ion channels. Every cell contains several types of receptors, G proteins, and effectors. The specificity with which G protein subunits interact with receptors and effectors defines the range of responses a cell is able to make to an external signal. Thus, the G proteins act as a critical control point that determines whether a signal spreads through several pathways or is focused to a single pathway. In this review, I will summarize some features of the structure and function of mammalian G protein subunits, discuss the role of both alpha and beta gamma subunits in regulation of effectors, the role of the beta gamma subunit in macromolecular assembly, and the mechanisms that might make some responses extremely specific and others rather diffuse.

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Purification of β,γ Subunits of Guanine Nucleotide Binding Proteins

Cooney¹,

Keenan²

1990

Receptor Purification

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The Role of β, γ-Subunits of Guanine Nucleotide Binding Proteins in Control of a Reconstituted Signal Transmission Chain Containing Purified Components of the Adenylate Cyclase System

Cited by 14 publications

References 43 publications

Physiological Regulation of G Protein-Linked Signaling

Physiological Regulation of G Protein-Linked Signaling

G proteins: Critical control points for transmembrane signals

Purification of β,γ Subunits of Guanine Nucleotide Binding Proteins

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