The Role of ΔFosB on the Pro-survival Effect of PTHrP in Goat Mammary Epithelial Cells

Yao, Xiaotong; Li, Lihui; Zheng, Heng

doi:10.1007/s12010-016-2126-4

Cited by 4 publications

(2 citation statements)

References 22 publications

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“…Western blot was performed as described previously (Li et al, 2016). Cells were collected from different treatment groups, pelleted them by centrifugation, and lysed them in radio immunoprecipitation assay (RIPA) buffer.…”

Section: Quantitative Real-time Pcr and Western Blotmentioning

confidence: 99%

Serotonin induces parathyroid hormone-related protein in goat mammary gland

Zang

Zhang

et al. 2018

Journal of Animal Science

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During lactation, large amounts of calcium are exported from the mammary gland into milk to ensure skeletal growth of the offspring. Recent studies revealed that serotonin (5-HT) is essential to stimulate skeletal calcium resorption for milk synthesis. Our objective was to explore the correlation between circulating 5-HT and serum calcium and parathyroid hormone-related protein (PTHrP) concentrations around parturition in dairy goats. We also investigated the effect of 5-HT on PTHrP expression in cultured primary goat mammary epithelial cells (GMEC). Blood samples of multiparous Guanzhong dairy goats were collected on day -5 to 3 postpartum for analysis of serum concentrations of calcium, 5-HT, and PTHrP. Results revealed that from day -3 to 0 postpartum serum calcium and 5-HT concentrations decreased progressively, but serum PTHrP concentration only had a sharp drop in the postpartum period sampled. Correlation analysis of circulating 5-HT and serum calcium and PTHrP concentrations on day 1 and 2 postpartum revealed that low serum 5-HT concentration was positively correlated with serum total calcium or PTHrP concentration. By knocking down tryptophan hydroxylase-1 (TPH1) or adding 5-hydroxytryptophan (5-HTP) to decrease or increase the levels of 5-HT in GMEC, we observed that 5-HTP increased PTHrP expression in a dose-dependent manner and siTPH1 decreased PTHrP protein expression. Furthermore, 5-HT increased mRNA abundance of calcium-sensing receptor (CaSR) in a dose-dependent manner and decreased the expression of plasma membrane Ca2+ ATPase-1 (PMCA1). Taken together, 5-HT seems to induce PTHrP expression in goat mammary cells during and after parturition. These findings suggest that increasing 5-HT biosynthesis could be a potential therapeutic target for prevention of hypocalcemia in dairy goats.

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Section: Quantitative Real-time Pcr and Western Blotmentioning

confidence: 99%

Serotonin induces parathyroid hormone-related protein in goat mammary gland

Zang

Zhang

et al. 2018

Journal of Animal Science

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“…Part of this amount is released into the milk and an aliquot exceptionally gets into the bloodstream where it triggers the mobilization of skeletal calcium to be delivered to the breast for milk production . Li et al have recently reported that overexpression of PTHrP via recombinant adenovirus transduction promotes proliferation and restrain apoptosis of goat mammary epithelial cells by regulating the expression of key genes such as cyclin‐D1, PCNA, Bcl‐2, and Bcl‐xl, demonstrating that this factor plays an important role in the control of the survival of this cytotype.…”

Section: Introductionmentioning

confidence: 99%

Mid‐region parathyroid hormone‐related protein is a genome‐wide chromatin‐binding factor that promotes growth and differentiation of HB2 epithelial cells from the human breast

et al. 2018

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Human parathyroid hormone‐related protein (PTHrP) is a polyhormone that undergoes proteolytic cleavage producing smaller peptides which exert diversified biological effects. PTHrP signalization is prominently involved in breast development and physiology, but the studies have been focused onto either N‐terminal species or full‐length protein introduced by gene transfer techniques. Our present work investigates for the first time the effect of the mid‐region PTHrP secretory form, that is, the fragment [38–94], on HB2 non‐tumoral breast epithelial cells. We examined viability/proliferation of cells grown in PTHrP‐containing media supplemented with different serum concentration and on different substrates, extending our investigation to check whether (a) by analogy with MDA‐MB231 cells, also HB2 cell chromatin possesses genome‐wide binding sites for mid‐region PTHrP, and (b) the peptide is endowed with modulating properties toward the expression of proliferation/differentiation signatures by HB2 cells. Our results indicate that mid‐region PTHrP acts as a cell growth/differentiation stimulator in routine and “nutrient stress” culture conditions, accordingly reprogramming gene expression, and is able to bind to cytogenetic preparations from HB2 cells. This supports the concept that the physiological mechanisms involving PTHrP during breast development may include mature processed forms of the protein different from the N‐terminal fragment. © 2018 BioFactors, 45(2):279–288, 2019

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Increasing serotonin concentrations alter calcium metabolism in periparturient dairy goats

Zhang

Liu

et al. 2022

Journal of Animal Science

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Due to the large amounts of calcium transferred to milk from mammary glands, periparturient dairy goats face challenges with calcium metabolism disorder and hypocalcemia. Serotonin (5-hydroxytryphtamine, 5-HT), the product of 5-hydroxy-l-tryptophan (5-HTP) catalysed by tryptophan hydroxylase 1 (TPH1), is a multifunctional monoamine thought to be a homeostatic regulator of the animal. The objective of the current study was to investigate the effects and underlying mechanisms of intramuscular 5-HTP injections on calcium homeostasis in the goat mammary glands. In the in vivo experiment, thirty multiparous Guanzhong dairy goats were randomly assigned to two groups, one group was injected with 5-HTP intramuscularly and the other group was injected with normal saline. From the first 10 days of the expected date for delivery, 5-HTP or saline was injected into goats through the shoulder muscle every morning before feeding, with a dose of 1 mg/kg per body weight. In the in vitro experiment, goat mammary epithelial cells (GMEC) were treated with 100 μM 5-HT for the evaluation of 5-HT in calcium transportation. The results demonstrated that 5-HTP treatment had no effect on the basic composition of colostrum (P > 0.05) but increased the serum 5-HT concentrations on d -5, -4, -3 and d 5 relative to parturition (P <0.05). The 5-HTP injection group had greater serum calcium concentration on d 4 and greater serum parathyroid hormone related protein (PTHrP) on d -5, -4, -1, 3, 4, and 5 compared to the saline injection group (P <0.05). It was further confirmed that 5-HT could increase intracellular calcium levels by increasing PTHrP and decreasing plasma-membrane Ca2+-ATPases1(PMCA1) in GMEC (P <0.05). In conclusion, 5-HTP treatment in multiparous goats during the transition period form pregnancy to lactation is a feasible way to protect goats form calcium metabolism disorder.

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The Role of ΔFosB on the Pro-survival Effect of PTHrP in Goat Mammary Epithelial Cells

Cited by 4 publications

References 22 publications

Serotonin induces parathyroid hormone-related protein in goat mammary gland

Serotonin induces parathyroid hormone-related protein in goat mammary gland

Mid‐region parathyroid hormone‐related protein is a genome‐wide chromatin‐binding factor that promotes growth and differentiation of HB2 epithelial cells from the human breast

Increasing serotonin concentrations alter calcium metabolism in periparturient dairy goats

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