The Roles of Catabolic Factors in the Development of Osteoarthritis

Blasioli, Dominick J.; Kaplan, David L.

doi:10.1089/ten.teb.2013.0377

Cited by 80 publications

(67 citation statements)

References 94 publications

(90 reference statements)

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“…Moreover, high levels of IL1β RA have been found in the SF of OA patients with a 4000× ratio versus IL1β. As suggested by Blasioli and Kaplan (), in in vitro culture systems the concentrations of IL1β, “several orders of magnitude higher than what is found in the OSF,” are needed to reproduce an environment similar to OA.…”

Section: Discussionmentioning

confidence: 99%

Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models

Pagani

Borsari

Veronesi

et al. 2016

Journal Cellular Physiology

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Primarily, to compare the behavior of human mesenchymal stem cells (MSCs) derived from bone marrow (hBMSCs) and adipose tissue (hADSCs) in an osteoarthritic (OA) microenvironment; secondly, to investigate the reaction of these cell types in two alternative in vitro culture systems, obtained by using TNFα and/or IL1β as inflammation mediators, or by using synovial fluid harvested by OA patients (OSF) to simulate the complex inflamed knee microenvironment. 3D micromass cultures of hBMSCs or hADSCs were grown in chondrogenic medium (CTR), in the presence of TNFα and/or IL1β, or synovial fluid from OA patients. After 1 month of culture, the chondrogenic differentiation of micromasses was evaluated by gene expression, matrix composition, and organization. Both hMSCs types formed mature micromasses in CTR, but a better response of hADSCs to the inflammatory environment was documented by micromass area and Bern score evaluations. The addition of OSF elicited a milder reaction than with TNFα and/or IL1β by both cell types, probably due to the presence of both catabolic and protective factors. In particular, SOX9 and ACAN gene expression and GAG synthesis were more abundant in hADSCs than hBMSCs when cultured in OSF. The expression of MMP1 was increased for both hMSCs in inflammatory conditions, but in particular by hBMSCs. hADSCs showed an increased chondrogenic potential in inflammatory culture systems, suggesting a better response of hADSCs in the OA environment, thus underlining the importance of appropriate in vitro models to study MSCs and potential advantages of using these cells for future clinical applications. J. Cell. Physiol. 232: 1478-1488, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 99%

Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models

Pagani

Borsari

Veronesi

et al. 2016

Journal Cellular Physiology

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“…Human cartilage explants were cultured in 10 ng/ml TNF-α to mimic cartilage degradation in vitro, as TNF-α is a well-accepted inducer of catabolism in cartilage (22). KOR signaling was activated by adding 1 μM dynorphin A in the culture medium.…”

Section: Oprk1mentioning

confidence: 99%

Kappa opioid receptor signaling protects cartilage tissue against posttraumatic degeneration

Wu¹,

Zhang²,

Shkhyan³

et al. 2017

JCI Insight

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“…Inflammation within the joints is one of the main contributors to OA [92]. Pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α) interleukin (IL)-1 and IL-6) and cytokine-inducible cyclooxygenase-2 (COX-2), matrix metalloproteinases (MMPs), nitric oxide (NO), and reactive oxygen species (ROS) are highly upregulated in OA [93].…”

Section: Mirnas In Inflammation and Matrix Degradation In Osteoarthritismentioning

confidence: 99%

The Role of MicroRNAs and Their Targets in Osteoarthritis

2016

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MicroRNA regulation and expression has become an emerging field in determining the mechanisms regulating a variety of inflammation-mediated diseases. Recent studies have focused on specific microRNAs that are differentially expressed in case of osteoarthritis. Furthermore, several targets of these miRNAs important in disease progression have also been identified. In this review, we focus on microRNA biogenesis, regulation, detection, and quantification with an emphasis on cellular localization and how these concepts may be linked to disease processes such as osteoarthritis. Next, we review the relationship of specific microRNAs to certain features and risk factors associated with osteoarthritis such as inflammation, obesity, autophagy, and cartilage homeostasis. We also identify selected microRNAs that are differentially expressed in osteoarthritic tissue, but have unidentified targets and functions in the disease pathogenesis. Lastly, we highlight the potential use of microRNAs for therapeutic purposes, and also point to certain remedies that regulate microRNA expression.

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The Roles of Catabolic Factors in the Development of Osteoarthritis

Cited by 80 publications

References 94 publications

Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models

Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models

Kappa opioid receptor signaling protects cartilage tissue against posttraumatic degeneration

The Role of MicroRNAs and Their Targets in Osteoarthritis

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