The roles of FgPEX2 and FgPEX12 in virulence and lipid metabolism in Fusarium graminearum

Wang, Lina; Zhang, Li; Liu, Chunjie; Sun, Shuang; Liu, Aixin; Liang, Yan; Yu, Jinfeng

doi:10.1016/j.fgb.2019.103288

Cited by 16 publications

(16 citation statements)

References 52 publications

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“…In this study, the PEX22-like mutant showed a significant reduction in disease severity. In addition, similar to FgPEX1, FgPEX2, FgPEX3, FgPEX4, FgPEX10, FgPEX12, FgPEX13, FgPEX14, and FgPEX33, we found that toxin production, which is known to be essential for the virulence of F. graminearum (Desjardins et al, 1993;Chen et al, 2018;Kong et al, 2019;Zhang et al, 2019a,b;Wang et al, 2020), was obviously reduced in the FgPEX22 deletion strain. Similarly, most peroxins in M. oryzae, except for PEX5, have critical roles in virulence (Wang et al, 2007(Wang et al, , 2019Goh et al, 2011;Chen et al, 2016;Li et al, 2017).…”

Section: Discussionsupporting

confidence: 74%

“…Herein, the PEX22-like mutant exhibited defects in sexual and asexual reproduction, producing less perithecium and conidiation formation and abnormal conidiation morphologies. Similar results show that mutants with deletion of any of seven other FgPEX genes, FgPEX1, FgPEX2, FgPEX4, FgPEX5, FgPEX6, FgPEX7, and FgPEX12, form normal perithecia with mature ascospores; however, the production of perithecia in these mutants is reduced compared to that in the WT strain (Min et al, 2012;Zhang et al, 2019a;Wang et al, 2020). In contrast, in Podospora anserine, mutants with PEX1, PEX4, PEX6, PEX8, PEX22, or PEX26 deleted are sterile (Suasteolmos et al, 2018).…”

Section: Discussionsupporting

confidence: 70%

“…There are several peroxins in F. graminearum involved in the peroxisome life cycle that have been characterized. For instance,FgPEX1,FgPEX2,FgPEX4,FgPEX5,FgPEX6,FgPEX7,FgPEX10,FgPEX12,FgPEX13,FgPEX14,and FgPEX33 are involved in mycotoxin biosynthesis, pathogenicity, and pexophagy (Min et al, 2012;Chen et al, 2018;Zhang et al, 2019a,b;Wang et al, 2020). In Magnaporthe oryzae, MoPEX5, MoPEX6, MoPEX7, MoPEX14, MoPEX19, and MoPEX11 family peroxins are involved in matrix protein import and peroxisomal fission processes (Deng et al, 2013;Wang et al, 2013Wang et al, , 2015Li et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Peroxin FgPEX22-Like Is Involved in FgPEX4 Tethering and Fusarium graminearum Pathogenicity

et al. 2021

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Peroxisomes are essential organelles that play important roles in a variety of biological processes in eukaryotic cells. To understand the synthesis of peroxisomes comprehensively, we identified the gene FgPEX22-like, encoding FgPEX22-like, a peroxin, in Fusarium graminearum. Our results showed that although FgPEX22-like was notably different from other peroxins (PEX) in Saccharomyces cerevisiae, it contained a predicted PEX4-binding site and interacted with FgPEX4 as a rivet protein of FgPEX4. To functionally characterize the roles of FgPEX22-like in F. graminearum, we performed homologous recombination to construct a deletion mutant (ΔPEX22-like). Analysis of the mutant showed that FgPEX22-like was essential for sexual and asexual reproduction, fatty acid utilization, pathogenicity, and production of the mycotoxin deoxynivalenol. Deletion of FgPEX22-like also led to increased production of lipid droplets and decreased elimination of reactive oxygen species. In addition, FgPEX22-like was required for the biogenesis of Woronin bodies. Taken together, our data demonstrate that FgPEX22-like is a peroxin in F. graminearum that interacts with PEX4 by anchoring PEX4 at the peroxisomal membrane and contributes to the peroxisome function in F. graminearum.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

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Peroxin FgPEX22-Like Is Involved in FgPEX4 Tethering and Fusarium graminearum Pathogenicity

et al. 2021

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“…In many fungi, it is known that peroxisome biogenesis affects sexual reproduction (Peraza Reyes and Berteaux-Lecellier, 2013). Although several PEX genes encoding peroxin required for sexual development in F. graminearum (Min et al, 2012;Kong et al, 2019;Wang et al, 2020), deletion of FPO1 did not affect the expression of these genes during both vegetative and early sexual developmental stages (Table S1). The conservation of FPO1 homologues among the subphylum Pezizomycotina (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…This fungus utilizes both mitochondrial and peroxisomal β-oxidation pathways to degrade and utilize fatty acids required for successful sexual reproduction (Bonnet et al, 2006;Boisnard et al, 2009). Accordingly, several genes encoding peroxins (PEX) and glyoxylate cycle genes, which are required for fatty acid utilization, are important for sexual development in F. graminearum (Lee et al, 2009b;Min et al, 2012;Wang et al, 2020). Moreover, peroxisomal fatty acid metabolisms provide signal molecules to induce proper reproduction processes.…”

Section: Introductionmentioning

confidence: 99%

The novel bZIP transcription factor Fpo1 negatively regulates perithecial development by modulating carbon metabolism in the ascomycete fungus Fusarium graminearum

Shin

Bui

Kim

et al. 2020

Environmental Microbiology

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Summary Fungal sexual reproduction requires complex cellular differentiation processes of hyphal cells. The plant pathogenic fungus Fusarium graminearum produces fruiting bodies called perithecia via sexual reproduction, and perithecia forcibly discharge ascospores into the air for disease initiation and propagation. Lipid metabolism and accumulation are closely related to perithecium formation, yet the molecular mechanisms that regulate these processes are largely unknown. Here, we report that a novel fungal specific bZIP transcription factor, F. graminearum perithecium overproducing 1 (Fpo1), plays a role as a global transcriptional repressor during perithecium production and maturation in F. graminearum. Deletion of FPO1 resulted in reduced vegetative growth, asexual sporulation and virulence and overproduced perithecium, which reached maturity earlier, compared with the wild type. Intriguingly, the hyphae of the fpo1 mutant accumulated excess lipids during perithecium production. Using a combination of molecular biological, transcriptomic and biochemical approaches, we demonstrate that repression of FPO1 after sexual induction leads to reprogramming of carbon metabolism, particularly fatty acid production, which affects sexual reproduction of this fungus. This is the first report of a perithecium‐overproducing F. graminearum mutant, and the findings provide comprehensive insight into the role of modulation of carbon metabolism in the sexual reproduction of fungi.

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The nuclear pore protein Nup2 is essential for growth and development, stress response, pathogenicity and deoxynivalenol biosynthesis in Fusarium graminearum

Wang,

Zhang,

Zhao

et al. 2024

Pest Management Science

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BACKGROUNDWheat is an important grain crop that has been under serious threat from Fusarium graminearum. Nup2, a member of the nuclear pore complex, plays an important role in regulating eukaryotic nuclear protein transport and participates in gene regulation. Dissecting the function of nuclear pore proteins in pathogenic fungi may provide effective targets for novel fungicides.RESULTSMutants exhibited nutritional growth defects, asexual/sexual developmental abnormalities. Deficiency of FgNup2 resulted in increased resistance of Fusarium graminearum to cell wall disruptors and increased sensitivity to metal ions. Pathogenicity analyses showed that the mutant was significantly less virulent on flowering wheat ears, consistent with the observed decrease in deoxynivalenol (DON) production. Furthermore, we showed that FgNup2 interacts synergistically with FgTri6, a transcription factor of the TRI family, to regulate the expression of toxin‐producing genes, which, in turn, affects the biosynthesis of DON and related toxins.CONCLUSIONThis study revealed that FgNup2 plays important roles in the growth and development, cell wall integrity, stress response, pathogenicity, and DON synthesis of F. graminearum. © 2024 Society of Chemical Industry.

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The roles of FgPEX2 and FgPEX12 in virulence and lipid metabolism in Fusarium graminearum

Cited by 16 publications

References 52 publications

Peroxin FgPEX22-Like Is Involved in FgPEX4 Tethering and Fusarium graminearum Pathogenicity

Peroxin FgPEX22-Like Is Involved in FgPEX4 Tethering and Fusarium graminearum Pathogenicity

The novel bZIP transcription factor Fpo1 negatively regulates perithecial development by modulating carbon metabolism in the ascomycete fungus Fusarium graminearum

The nuclear pore protein Nup2 is essential for growth and development, stress response, pathogenicity and deoxynivalenol biosynthesis in Fusarium graminearum

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