The roles of haemocytes and the lymphoid organ in the clearance of injected Vibrio bacteria in Penaeus monodon shrimp

Braak, C.B.T. van de; Botterblom, M.H.A.; Taverne, Nico; Muiswinkel, W.B. van; Rombout, J.H.W.M.; Knaap, W.P.W. van der

doi:10.1006/fsim.2002.0409

Cited by 170 publications

(134 citation statements)

References 32 publications

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“…When bacteria cross the barrier of the cuticle, the immune system acts by quickly reducing the concentration of circulating microrganisms that fix in the shrimp organs (Van de Braak et al, 2002). It was observed in this study thnat bacterial concentration in the hepatopancreas was higher than in the hemolymph 10h after challenge.…”

Section: Total Vibrio Countmentioning

confidence: 50%

“…On the other hand, THC of shrimp injected with SSS was not different between the groups, but higher than the V. harveyi challenged group (Table 1). This result seems to be associated with an inflammatory response of hemocytes that leave the circulation and migrate to the site of injection (Van de Braak et al, 2002). Additionally, hemocytes can aggregate into haemocytic nodules in which cell adhesion molecules, such as peroxinectin, apprehend microorganisms between them (Jiravanichpaisal et al, 2006) and then are mechanically eliminated from the circulation through the gills (Martin et al, 2000).…”

Section: Total Vibrio Countmentioning

confidence: 99%

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Effect of probiotic supplemented diet on marine shrimp survival after challenge with Vibrio harveyi

Vieira

Buglione

Mouriño

et al. 2010

Arq. Bras. Med. Vet. Zootec.

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The effect of a Lactobacillus plantarum-supplemented diet on shrimp growth, digestive tract bacterial microbiota, survival, and some hemato-immunological parameters after an experimental challenge with Vibrio harveyi was studied. No difference (P>0.05) was observed in shrimp survival (80±6%) and final weight (6.63±0.56g) after 60 days feeding trial. Total bacteria count and Vibrio spp. count in the digestive tract were not diferent (P>0.05) until day 40, but they were lower (P<0.05) in the probiotic-supplemented group on day 60. Total lactic bacteria in the shrimp digestive tract was higher after day 20 in the probiotic-supplemented group. Ten hours after V. harveyi challenge, survival of the probiotic-supplemented group (65.7±2.9%) was higher (P>0.05) than the control group (39.9±4.4%). Bacterial counts in hemolymph and hepatopancreas was lower (P<0.05) in the probiotic-supplemented group than in the control group after V. harveyi challenge. Total hemocyte count and serum agglutination activity were higher (P>0.05) in the probiotic-supplemented group after challenge with V. harveyi. Probiotic-supplemented diet modifies shrimp digestive tract bacterial microbiota, increasing resistance to V. harveyi infection. Keywords: Litopenaeus vannamei, Lactobacillus plantarum, intestinal microbiota, immunostimulation RESUMO Avaliou-se o efeito de uma dieta suplementada com Lactobacillus plantarum na engorda de

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Section: Total Vibrio Countmentioning

confidence: 50%

Section: Total Vibrio Countmentioning

confidence: 99%

Effect of probiotic supplemented diet on marine shrimp survival after challenge with Vibrio harveyi

Vieira

Buglione

Mouriño

et al. 2010

Arq. Bras. Med. Vet. Zootec.

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“…In the shrimp hemolymph, lysozyme expression is confined to the hemocytes. The lymphoid organ is a primary site of bacterial accumulation and bacteriostasis and accommodates hemocytes that in turn facilitates bacterial phagocytosis (37,38). Shrimp gills meanwhile play a multifunctional role and were reported to be a site of the formation of hemocyte nodules during foreign particle injection H, hemocytes; L, lymphoid organ) at day 0 and after dsLYZ injection.…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis of C-type Lysozyme in Penaeid Shrimp

Kaizu

Fagutao

Kondo

et al. 2011

Journal of Biological Chemistry

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Background: Lysozyme is ubiquitous in many organisms and functions primarily in bacterial lysis. Results: The absence of lysozyme in shrimp resulted in the increase of bacteria in the hemolymph leading to mortality. Conclusion: Lysozyme is essential to shrimp survival particularly in regulating bacterial communities in the hemolymph. Significance: A clear understanding of the immune effectors in shrimp, such as AMPs, is vital in ensuring resistance against bacterial pathogens.

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“…The lymphoid organ and intestine of penaeid shrimps are thought to have immune function. The lymphoid organ exerts bacteriostatic effects, and is suggested to be the major phagocytic organ in shrimp ( Van de Braak et al 2002;Burgents et al 2005). The intestine is a favourable site for invasion of pathogens carried in water, food, and sediment (Jayabalan et al 1982).…”

mentioning

confidence: 99%

Expression of innate immunity genes in kuruma shrimp Marsupenaeus japonicus after in vivo stimulation with garlic extract (allicin)

Tanekhy¹,

Fall

2015

Vet. Med.

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ABSTRACT:In recent times, attention has focused on immunostimulants and plant products which could have beneficial effects in disease control. At present, the application of immunostimulants has been considered a more effective approach to health management in aquaculture through the enhancement of immune capability and disease resistance in shrimp. Garlic possesses bactericidal property against bacteria and can inhibit the growth of protozoa as well as gregarine infection in cultured shrimp. However, its effect on viral disease infection has not been well studied and requires further investigation. Garlic can also stimulate shrimp haemocytes as determined by cellular immune responses (phagocytic activity, superoxide anion production and phenoloxidase activity) suggesting that the immune-stimulatory properties of garlic will be useful for improvement of shrimp health. Here, we determined the expression of the immune-related genes Penaeidin, Crustin, Lysozyme, Toll-like, and tumour necrosis factor in kuruma shrimp, upon stimulation with allicin extract. The expression of these factors was measured for the first time and was found to be elevated in intestine and lymphoid organ after in vivo stimulation for 3, 12, 24 and 48 h. We conclude that garlic can be used in shrimp culture as an alternative to antibiotics or chemotherapeutic agents; however, further research is needed under field conditions.

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The roles of haemocytes and the lymphoid organ in the clearance of injected Vibrio bacteria in Penaeus monodon shrimp

Cited by 170 publications

References 32 publications

Effect of probiotic supplemented diet on marine shrimp survival after challenge with Vibrio harveyi

Effect of probiotic supplemented diet on marine shrimp survival after challenge with Vibrio harveyi

Functional Analysis of C-type Lysozyme in Penaeid Shrimp

Expression of innate immunity genes in kuruma shrimp Marsupenaeus japonicus after in vivo stimulation with garlic extract (allicin)

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