The roles of HD-ZIP proteins in plant abiotic stress tolerance

Li, Yuxia; Yang, Zhongnian; Zhang, Yuanyuan; Guo, Jinjiao; Liu, Huan; Wang, Chengfeng; Wang, Baoshan; Han, Guoliang

doi:10.3389/fpls.2022.1027071

Cited by 33 publications

(19 citation statements)

References 164 publications

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“…HD-ZIP proteins were predicated to be transcription factors in plants [ 10 ]. A transcriptional activation assay was performed using the Gal4 binding domain (BD)-MsHDZ23 fusion protein and a constitutively expressed reporter gene containing four upstream Gal4 DNA-binding sites (Gal4 [4X]-D1-3[4X]-GUS; Figure 2 A).…”

Section: Resultsmentioning

confidence: 99%

“…Among these transcription factors, the HD-ZIP family is one of the prominently induced families. HD-ZIP proteins comprise a large family of plant-specific TFs, which plays a crucial role in the growth and development of plants and stress responses [ 10 ]. Transcriptome analysis revealed diverse expression patterns of HD-ZIP genes in response to abiotic stresses ( Figure S1 ).…”

Section: Discussionmentioning

confidence: 99%

“…TFs regulate abiotic stress signaling through pathways dependent or independent of ABA signaling [ 9 ]. The homeodomain-leucine zipper (HD-ZIP) TF family is of particular interest among the various TF families because of its crucial regulatory role in tolerating various abiotic stresses [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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MsHDZ23, a Novel Miscanthus HD-ZIP Transcription Factor, Participates in Tolerance to Multiple Abiotic Stresses

Liu,

Yu,

Deng

et al. 2024

IJMS

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The homeodomain-leucine zipper (HD-ZIP) transcription factors, representing one of the largest plant-specific superfamilies, play important roles in the response to various abiotic stresses. However, the functional roles of HD-ZIPs in abiotic stress tolerance and the underlying mechanisms remain relatively limited in Miscanthus sinensis. In this study, we isolated an HD-ZIP TF gene, MsHDZ23, from Miscanthus and ectopically expressed it in Arabidopsis. Transcriptome and promoter analyses revealed that MsHDZ23 responded to salt, alkali, and drought treatments. The overexpression (OE) of MsHDZ23 in Arabidopsis conferred higher tolerance to salt and alkali stresses compared to wild-type (WT) plants. Moreover, MsHDZ23 was able to restore the hb7 mutant, the ortholog of MsHDZ23 in Arabidopsis, to the WT phenotype. Furthermore, MsHDZ23-OE lines exhibited significantly enhanced drought stress tolerance, as evidenced by higher survival rates and lower water loss rates compared to WT. The improved drought tolerance may be attributed to the significantly smaller stomatal aperture in MsHDZ23-OE lines compared to WT. Furthermore, the accumulation of the malondialdehyde (MDA) under abiotic stresses was significantly decreased, accompanied by dramatically enhanced activities in several antioxidant enzymes, including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in the transgenic plants. Collectively, these results demonstrate that MsHDZ23 functions as a multifunctional transcription factor in enhancing plant resistance to abiotic stresses.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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MsHDZ23, a Novel Miscanthus HD-ZIP Transcription Factor, Participates in Tolerance to Multiple Abiotic Stresses

Liu,

Yu,

Deng

et al. 2024

IJMS

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“…In the analysis of the promoter regions of JcDHX genes, a diverse array of TFs spanning different families (Dof-type, BBR-BPC, HD-ZIP, AP2-ERF, WRKY, bHLH, and Myb-related) were identified, with particular emphasis on Dof-type TFs. These TFs, prominently featured in plant responses to abiotic stress [ 61 , 62 , 63 ], have demonstrated key roles in orchestrating stress-responsive gene expression. Dof-type TFs, which are characterized by a DNA-binding zinc finger, have emerged as critical regulators of plant environmental stress responses.…”

Section: Discussionmentioning

confidence: 99%

DEAD-Box RNA Helicase Family in Physic Nut (Jatropha curcas L.): Structural Characterization and Response to Salinity

da Silva,

Silva,

Ferreira-Neto

et al. 2024

Plants

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Helicases, motor proteins present in both prokaryotes and eukaryotes, play a direct role in various steps of RNA metabolism. Specifically, SF2 RNA helicases, a subset of the DEAD-box family, are essential players in plant developmental processes and responses to biotic and abiotic stresses. Despite this, information on this family in the physic nut (Jatropha curcas L.) remains limited, spanning from structural patterns to stress responses. We identified 79 genes encoding DEAD-box RNA helicases (JcDHX) in the J. curcas genome. These genes were further categorized into three subfamilies: DEAD (42 genes), DEAH (30 genes), and DExH/D (seven genes). Characterization of the encoded proteins revealed a remarkable diversity, with observed patterns in domains, motifs, and exon–intron structures suggesting that the DEAH and DExH/D subfamilies in J. curcas likely contribute to the overall versatility of the family. Three-dimensional modeling of the candidates showed characteristic hallmarks, highlighting the expected functional performance of these enzymes. The promoter regions of the JcDHX genes revealed potential cis-elements such as Dof-type, BBR-BPC, and AP2-ERF, indicating their potential involvement in the response to abiotic stresses. Analysis of RNA-Seq data from the roots of physic nut accessions exposed to 150 mM of NaCl for 3 h showed most of the JcDHX candidates repressed. The protein–protein interaction network indicated that JcDHX proteins occupy central positions, connecting events associated with RNA metabolism. Quantitative PCR analysis validated the expression of nine DEAD-box RNA helicase transcripts, showing significant associations with key components of the stress response, including RNA turnover, ribosome biogenesis, DNA repair, clathrin-mediated vesicular transport, phosphatidyl 3,5-inositol synthesis, and mitochondrial translation. Furthermore, the induced expression of one transcript (JcDHX44) was confirmed, suggesting that it is a potential candidate for future functional analyses to better understand its role in salinity stress tolerance. This study represents the first global report on the DEAD-box family of RNA helicases in physic nuts and displays structural characteristics compatible with their functions, likely serving as a critical component of the plant’s response pathways.

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“…For example, EgMYB1 and EgMYB2 could bind specifically to the promoter region of lignin synthesis gene such as Coenzyme A Reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) and further regulate secondary wall biosynthesis [ 12 , 37 , 38 ]. In addition, EcHB1 of the HD-ZIP Class II family TFs and LIM transcription factor were also found to enhance xylem cell wall biosynthesis [ 39 , 40 ]. However, there are still a large number of valuable genes with potential breeding need to be discovered.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of the transition from primary to secondary growth of vertical stem in Eucalyptus grandis

Zhou,

Zhang,

Chen

et al. 2024

BMC Plant Biol

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Eucalyptus was one of the most cultivated hardwood species worldwide, with rapid growth, good wood properties and a wide range of adaptability. Eucalyptus stem undergoes primary growth (longitudinal growth) followed by secondary growth (radial growth), which produces biomass that is an important source of energy worldwide. In order to better understand the genetic regulation of secondary growth in Eucalyptus grandis, Transcriptome analyses in stem segments along a developmental gradient from the third internode to the eleventh internode of E. grandis that spanned primary to secondary growth were carried out. 5,149 genes that were differentially expressed during stem development were identified. Combining the trend analysis by the Mfuzz method and the module-trait correlation analysis by the Weighted Gene Co-expression Network Analysis method, a total of 70 differentially expressed genes (DEGs) selected from 868 DEGs with high connectivity were found to be closely correlated with secondary growth. Results revealed that the differential expression of these DEGs suggests that they may involve in the primary growth or secondary growth. AP1, YAB2 TFs and EXP genes are highly expressed in the IN3, whereas NAC, MYB TFs are likely to be important for secondary growth. These results will expand our understanding of the complex molecular and cellular events of secondary growth and provide a foundation for future studies on wood formation in Eucalyptus.

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The roles of HD-ZIP proteins in plant abiotic stress tolerance

Cited by 33 publications

References 164 publications

MsHDZ23, a Novel Miscanthus HD-ZIP Transcription Factor, Participates in Tolerance to Multiple Abiotic Stresses

MsHDZ23, a Novel Miscanthus HD-ZIP Transcription Factor, Participates in Tolerance to Multiple Abiotic Stresses

DEAD-Box RNA Helicase Family in Physic Nut (Jatropha curcas L.): Structural Characterization and Response to Salinity

Transcriptome analysis of the transition from primary to secondary growth of vertical stem in Eucalyptus grandis

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