The roles of microRNAs in epigenetic regulation

Yao, Qian; Chen, Yuqi; Zhou, Xiang

doi:10.1016/j.cbpa.2019.01.024

Cited by 384 publications

(254 citation statements)

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“…The binding of miRNA to mRNA leads to mRNA degradation or translational repression, and thus miRNAs are negative regulators of gene expression . Through the post‐transcriptional regulation of gene expression, miRNAs are believed to regulate various biological processes, including proliferation, apoptosis, the cell cycle, and differentiation, that are involved in both physiological and pathological progress . Currently, accumulating evidence indicates that various miRNAs are dysregulated in cerebral ischaemia/reperfusion injury and can be potentially used as diagnostic and/or therapeutic targets .…”

Section: Introductionmentioning

confidence: 99%

Inhibition of microRNA‐148b‐3p alleviates oxygen‐glucose deprivation/reoxygenation‐induced apoptosis and oxidative stress in HT22 hippocampal neuron via reinforcing Sestrin2/Nrf2 signalling

et al. 2020

Clin Exp Pharma Physio

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MicroRNAs (miRNAs) have emerged as crucial regulators of neuronal injury during cerebral ischaemia/reperfusion injury. Various miRNAs are dysregulated during this pathological process; however, the precise role of these miRNAs in regulating neuronal injury remains largely unknown. In the current study, we explored the potential function of microRNA‐148b‐3p (miR‐148b‐3p) in regulating neuronal injury induced by oxygen‐glucose deprivation/reoxygenation (OGD/R) in vitro, a cellular model for mimicking cerebral ischaemia/reperfusion injury. We found that miR‐148b‐3p expression was significantly decreased in neurons in response to OGD/R exposure. Importantly, miR‐148b‐3p overexpression decreased cell viability and exacerbated apoptosis and reactive oxygen species (ROS) production in OGD/R‐exposed neurons. By contrast, miR‐148b‐3p inhibition improved cell viability and decreased apoptosis and ROS production in OGD/R‐exposed neurons. Notably, Sestrin2, a cytoprotective gene, was identified as a miR‐148b‐3p target gene. miR‐148b‐3p inhibition markedly increased Sestrin2 expression as well as the activation of nuclear factor erythroid‐2‐related factor 2 (Nrf2) antioxidant signalling. Moreover, silencing of Sestrin2 or Nrf2 significantly reversed the miR‐148‐3p‐inhibition‐mediated protective effect in OGD/R‐injured neurons. Overall, these results demonstrate that miR‐148b‐3p inhibition protects neurons from OGD/R‐induced apoptosis and ROS production through reinforcing Nrf2 antioxidant signalling via upregulation of Sestrin2. Our study indicates that the miR‐148b‐3p/Sestrin2/Nrf2 axis plays an important role in regulating neuronal injury and may serve as a potential therapeutic target for providing neuroprotection during cerebral ischaemia/reperfusion injury.

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Section: Introductionmentioning

confidence: 99%

Inhibition of microRNA‐148b‐3p alleviates oxygen‐glucose deprivation/reoxygenation‐induced apoptosis and oxidative stress in HT22 hippocampal neuron via reinforcing Sestrin2/Nrf2 signalling

et al. 2020

Clin Exp Pharma Physio

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“…The allelic loss, copy number aberrations, mutations, and methylations of CSMD1 have been detected in various malignant tumours such as head and neck tumor, colorectal cancers, liver cancer, and so on [5][6][7][8][9][10][11][12][13][14][15][16]. Besides, it has been proved that the change of microRNA expression and dysfunction play an important role in tumorigenesis and metastasis by regulating target genes and pathways, subsequently resulting in the alteration of proliferation, differentiation, apoptosis, invasion and metastasis of tumor cells [17][18][19]. Therefore, the molecular mechanism underlying the microRNAs-driven deregulation of CSMD1 contributing to GC metastases need to be urgently unraveled.…”

Section: Introductionmentioning

confidence: 99%

“…MicroRNA is a small single-stranded non-coding RNA, that is a post-transcriptional negative regulator that binds completely or partly to the complementary sites in the 3′-untranslated region (3′UTR) of target mRNAs [17][18][19]. Accumulating evidence has shown that miRNAs can modulate tumor growth, metastasis, and progression by regulating multiple target genes [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Deregulation of CSMD1 targeted by microRNA-10b drives gastric cancer progression through the NF-κB pathway

et al. 2019

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Aim:This study aimed to investigate the oncogenic activity of microRNA-10b by targeting CUB and sushi multiple domains protein 1 (CSMD1) in human gastric cancer (GC) and the underlying mechanisms. Methods: The expression of CSMD1 in human GC tissues was evaluated by real-time reverse transcription polymerase chain reaction (RT-PCR), immunoblotting, and immunohistochemical analysis. The expressive abundance of microRNA-10b was detected by stem-loop RT-PCR. Molecular and cellular techniques, including lentiviral vector-mediated knockdown or overexpression, were used to elucidate the effect of microRNA-10b on the expression of CSMD1. Results: CSMD1 was targeted and downregulated by microRNA-10b in human GC tissues and cells, and the down-regulated expression of CSMD1 contributed to poor survival. The knockdown of microRNA-10b expression inhibited cell proliferation in GC cells in vitro and tumor growth in vivo. The inhibition of microRNA-10b expression repressed invasion and migration of HGC27 cells and retarded GC cells metastasis to the liver in Balb/c nude mice. The up-regulated expression of microRNA-10b promoted the proliferation and metastasis of MKN74 cell in vitro. Intratumoral injection of microRNA-10b mimic also promoted the growth and metastasis of tumor xenografts in Balb/c nude mice. Mechanistically, microRNA-10b promoted the invasion and metastasis of human GC cells through inhibiting the expression of CSMD1, leading to the activation of the nuclear factor-κB (NF-κB) pathway that links inflammation to carcinogenesis, subsequently resulting in the upregulation of c-Myc, cyclin D1 (CCND1), and epithelial-mesenchymal transition (EMT) markers. Conclusions: The findings established that microRNA-10b is an oncomiR that drives metastasis. Moreover, a set of critical tumor suppressor mechanisms was defined that microRNA-10b overcame to drive human GC progression.

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“…MicroRNAs (miRNAs) are a class of endogenously small noncoding RNAs with approximately 18-25 nucleotides in length [11], and may inhibit protein translation or degrading the polypeptides via binding to the untranslated regions of mRNA [12]. Previous studies [13][14][15] have found that circulating miRNAs can be considered as potential biomarkers for detecting kinds of diseases, including autoimmune diseases.…”

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confidence: 99%

Diagnostic significance of circulating miRNAs in systemic lupus erythematosus

et al. 2019

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BackgroundIn recent years, many studies focused on the association between the microRNAs (miR-NAs) and the risk of systemic lupus erythematosus (SLE), especially miRNA-21 (miR-21). We aimed to investigate the role of circulating miRNAs, especially the miR-21, as a biomarker in detecting SLE. MethodsWe searched PubMed, EMBASE, the Cochrane Central Register of Controlled Trials, and China National Knowledge Infrastructure through Mar 3th, 2019. We performed this metaanalysis in a fixed/random-effect model using Meta-disc 1.4 and STATA 15.1. ResultsA total of 17 relevant studies were eligible to analyze pooled accuracy. The overall performance of total mixed miRNAs (TmiRs) detection was: pooled sensitivity, 0.71 (95% confidence interval [CI], 0.69 to 0.72); pooled specificity, 0.81 (95%CI, 0.79 to 0.83); and area under the summary receiver operating characteristic curves value (SROC), 0.8797. The miR-21 detection was: pooled sensitivity, 0.68 (95%CI, 0.62 to 0.74); pooled specificity, 0.77 (95%CI, 0.69 to 0.84); and SROC, 0.8281. The meta-regression analysis showed that the type of samples was the sources of heterogeneity. The subgroup analysis suggested that detection in plasma group had the largest AUC of SROC in all the subgroups: pooled sensitivity, 0.8 (95%CI, 0.78 to 0.82); pooled specificity, 0.83 (95%CI, 0.8 to 0.86); and SROC, 0.9068. ConclusionsOur meta-analysis demonstrated that circulating miRNAs might be potential novel biomarkers for detecting SLE, especially miR-21. Moreover, plasma is recommended as the clinical specimen for diagnostic detection.

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The roles of microRNAs in epigenetic regulation

Cited by 384 publications

References 50 publications

Inhibition of microRNA‐148b‐3p alleviates oxygen‐glucose deprivation/reoxygenation‐induced apoptosis and oxidative stress in HT22 hippocampal neuron via reinforcing Sestrin2/Nrf2 signalling

Inhibition of microRNA‐148b‐3p alleviates oxygen‐glucose deprivation/reoxygenation‐induced apoptosis and oxidative stress in HT22 hippocampal neuron via reinforcing Sestrin2/Nrf2 signalling

Deregulation of CSMD1 targeted by microRNA-10b drives gastric cancer progression through the NF-κB pathway

Diagnostic significance of circulating miRNAs in systemic lupus erythematosus

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