The roles of Pseudomonas aeruginosa extracellular polysaccharides in biofouling of reverse osmosis membranes and nitric oxide induced dispersal

Barnes, Robert J.; Bandi, Ratnaharika R.; Chua, Felicia; Low, Jiun Hui; Aung, Theingi; Barraud, Nicolas; Fane, Anthony G.; Kjelleberg, Staffan; Rice, Scott A.

doi:10.1016/j.memsci.2014.04.046

Cited by 41 publications

(29 citation statements)

References 43 publications

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“…For PAO1 experiments, a nutrient broth (NB) (Difco, BD) stock solution (8 g liter Ϫ1 ) was used to provide an average nutrient concentration of 24 mg liter Ϫ1 . This is a similar concentration to previous biofouling studies in laboratory-scale RO systems (34)(35)(36)(37) . The system was allowed to mix for a further 1.5 h prior to the start of the experiment.…”

Section: Methodssupporting

confidence: 86%

“…A recent study showed that the biofouling rate is dependent on both the quantity of EPS components and the surface coverage of the membrane biofilm, rather than the number of bacterial cells present (34). A lack of biofilm polysaccharides combined with areas of unfouled membrane dramatically reduced any hydraulic resistance to permeate flow, thus delaying the rate of biofouling (34). This study showed similar results, where a reduction in EPS constituents (protein and polysaccharide) and a decreased biofilm surface coverage after NO treatment led to significantly lower biofouling rates.…”

Section: Discussionmentioning

confidence: 99%

“…The design ( Fig. 1) and operation have been previously described (34) and represent conditions that simulate typical large-scale RO processes. Each 10-liter feed tank was equipped with a stirrer (IKA; model Eurostar) and was cooled using a chiller (PolyScience) to maintain the feed solution at 23 Ϯ 1°C (Fig.…”

Section: Methodsmentioning

confidence: 99%

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Nitric Oxide Treatment for the Control of Reverse Osmosis Membrane Biofouling

Barnes

Low

Bandi

et al. 2015

Appl Environ Microbiol

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f Biofouling remains a key challenge for membrane-based water treatment systems. This study investigated the dispersal potential of the nitric oxide (NO) donor compound, PROLI NONOate, on single-and mixed-species biofilms formed by bacteria isolated from industrial membrane bioreactor and reverse osmosis (RO) membranes. The potential of PROLI NONOate to control RO membrane biofouling was also examined. Confocal microscopy revealed that PROLI NONOate exposure induced biofilm dispersal in all but two of the bacteria tested and successfully dispersed mixed-species biofilms. The addition of 40 M PROLI NONOate at 24-h intervals to a laboratory-scale RO system led to a 92% reduction in the rate of biofouling (pressure rise over a given period) by a bacterial community cultured from an industrial RO membrane. Confocal microscopy and extracellular polymeric substances (EPS) extraction revealed that PROLI NONOate treatment led to a 48% reduction in polysaccharides, a 66% reduction in proteins, and a 29% reduction in microbial cells compared to the untreated control. A reduction in biofilm surface coverage (59% compared to 98%, treated compared to control) and average thickness (20 m compared to 26 m, treated compared to control) was also observed. The addition of PROLI NONOate led to a 22% increase in the time required for the RO module to reach its maximum transmembrane pressure (TMP), further indicating that NO treatment delayed fouling. Pyrosequencing analysis revealed that the NO treatment did not significantly alter the microbial community composition of the membrane biofilm. These results present strong evidence for the application of PROLI NONOate for prevention of RO biofouling.

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Section: Methodssupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

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Nitric Oxide Treatment for the Control of Reverse Osmosis Membrane Biofouling

Barnes

Low

Bandi

et al. 2015

Appl Environ Microbiol

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“…Indeed, even with continuous dosing, biofouling formation has been observed [2]. In recent years, research studies investigating membrane biofouling control have focused on optimisation of pre-treatment for the limitation of nutrients in feed water [7,11], development of novel membrane materials (chlorine resistant [12] or anti-fouling [13]), determination of novel biocides such as DBNPA [14] or nitric oxide [15] and development of novel biological methods such as inhibition of biofilm growth by quorum sensing, biomass dispersion by cell wall hydrolase or bacteriophage and enzymatic disruption [16,17]. Although some of these novel techniques are promising none of them have proved to dramatically improve biofouling control, and none can be implemented for full-scale plant operation in the medium term.…”

Section: Introductionmentioning

confidence: 99%

Biofouling and scaling control of reverse osmosis membrane using one-step cleaning-potential of acidified nitrite solution as an agent

Filloux

Wang

Pidou

et al. 2015

Journal of Membrane Science

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scaling control of reverse osmosis membrane using one-step cleaning-potential of acidified nitrite solution as an a g e n t , Journal of Membrane Science, http://dx.doi.org/10. 1016/j.memsci.2015.08.034 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractBiofouling is generally regarded as a major issue in reverse osmosis (RO) membrane filtration. Two-step chemical cleanings with alkaline and acidic agents are typically applied to restore the treatment capacity. In this study, the feasibility of one-step cleaning using free nitrous acid (FNA) was investigated as a novel low cost cleaning agent. The FNA cleaning solution was prepared by acidification of a sodium nitrite solution with hydrochloric acid.Seven fouled RO membranes collected from full-scale wastewater recycling and desalination plants were used to perform lab-scale cleaning trials. Membrane fouling characterisation revealed six of out of seven membranes were mainly bio-fouled, while one membrane was severely fouled by calcium carbonate. This study showed the feasibility of using FNA at pH 3.0 for biomass removal as well as for calcium carbonate scaling removal. The results from the lab-scale cleaning tests suggested that FNA can be used as a single cleaning agent for both biofouling and scaling removal. Cost analysis showed that FNA is a cost-effective solution for biofouling and scaling removal in RO filtration applications.

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“…aeruginosa, which is ubiquitous in soil and water, is one of the most prevalent biofouling strains in membrane systems and has been isolated from biofilms on water treatment membranes (12,13,15). It is used as a model bacterium for membrane-fouling studies because of its ability to form biofilms and because the genetic basis of its biofilm formation is well studied (40). Sphingomonads are another key biofouling organism in membrane systems; they colonize membrane and spacer surfaces rapidly and cover them with their extracellular polymeric substances (14).…”

Section: Deleterious Biofilm Formation Is Reduced and Permeate Flux Ismentioning

confidence: 99%

Living biofouling-resistant membranes as a model for the beneficial use of engineered biofilms

Wood

Guha

Tang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Membrane systems are used increasingly for water treatment, recycling water from wastewater, during food processing, and energy production. They thus are a key technology to ensure water, energy, and food sustainability. However, biofouling, the build-up of microbes and their polymeric matrix, clogs these systems and reduces their efficiency. Realizing that a microbial film is inevitable, we engineered a beneficial biofilm that prevents membrane biofouling, limiting its own thickness by sensing the number of its cells that are present via a quorum-sensing circuit. The beneficial biofilm also prevents biofilm formation by deleterious bacteria by secreting nitric oxide, a general biofilm dispersal agent, as demonstrated by both short-term dead-end filtration and long-term cross-flow filtration tests. In addition, the beneficial biofilm was engineered to produce an epoxide hydrolase so that it efficiently removes the environmental pollutant epichlorohydrin. Thus, we have created a living biofouling-resistant membrane system that simultaneously reduces biofouling and provides a platform for biodegradation of persistent organic pollutants.

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The roles of Pseudomonas aeruginosa extracellular polysaccharides in biofouling of reverse osmosis membranes and nitric oxide induced dispersal

Cited by 41 publications

References 43 publications

Nitric Oxide Treatment for the Control of Reverse Osmosis Membrane Biofouling

Nitric Oxide Treatment for the Control of Reverse Osmosis Membrane Biofouling

Biofouling and scaling control of reverse osmosis membrane using one-step cleaning-potential of acidified nitrite solution as an agent

Living biofouling-resistant membranes as a model for the beneficial use of engineered biofilms

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