The roles of SbcCD and RNaseE in the transcription of GAA·TTC repeats in Escherichia coli

Pan, Xuefeng; Ding, Yunfeng; Shi, Ling‐Feng

doi:10.1016/j.dnarep.2009.08.001

Cited by 4 publications

(3 citation statements)

References 37 publications

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“…This finding is consistent with the identification of HSEs in other organisms, where the GAA and TTC motifs are critical for the recognition of HSFs. This pattern is not unique to E. histolytica but is also observed in various other species, including D. melanogaster, S. cerevisiae, E. coli, Stratiomys singularia (S. singularior), and H. sapiens [27,[74][75][76].…”

Section: Discussionmentioning

confidence: 66%

Screening and Structural Characterization of Heat Shock Response Elements (HSEs) in Entamoeba histolytica Promoters

Dorantes-Palma,

Pérez-Mora,

Azuara-Liceaga

et al. 2024

IJMS

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Entamoeba histolytica (E. histolytica) exhibits a remarkable capacity to respond to thermal shock stress through a sophisticated genetic regulation mechanism. This process is carried out via Heat Shock Response Elements (HSEs), which are recognized by Heat Shock Transcription Factors (EhHSTFs), enabling fine and precise control of gene expression. Our study focused on screening for HSEs in the promoters of the E. histolytica genome, specifically analyzing six HSEs, including Ehpgp5, EhrabB1, EhrabB4, EhrabB5, Ehmlbp, and Ehhsp100. We discovered 2578 HSEs, with 1412 in promoters of hypothetical genes and 1166 in coding genes. We observed that a single promoter could contain anywhere from one to five HSEs. Gene ontology analysis revealed the presence of HSEs in essential genes for the amoeba, including cysteine proteinases, ribosomal genes, Myb family DNA-binding proteins, and Rab GTPases, among others. Complementarily, our molecular docking analyses indicate that these HSEs are potentially recognized by EhHSTF5, EhHSTF6, and EhHSTF7 factors in their trimeric conformation. These findings suggest that E. histolytica has the capability to regulate a wide range of critical genes via HSE-EhHSTFs, not only for thermal stress response but also for vital functions of the parasite. This is the first comprehensive study of HSEs in the genome of E. histolytica, significantly contributing to the understanding of its genetic regulation and highlighting the complexity and precision of this mechanism in the parasite’s survival.

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Section: Discussionmentioning

confidence: 66%

Screening and Structural Characterization of Heat Shock Response Elements (HSEs) in Entamoeba histolytica Promoters

Dorantes-Palma,

Pérez-Mora,

Azuara-Liceaga

et al. 2024

IJMS

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“…Several processes may be responsible for the observed alterations in colony morphology, including triplet repeats transcription from the lac Z promoter (Figure 1A) [26], DNA double strand breaks caused by formation of non-B secondary structures, such as non-H structure, which may be targeted by certain DNA structure specific nucleases [27], [28], or activation of the cryptic phageΦ 80 in the JM83 chromosome, leading to cell lysis.…”

Section: Resultsmentioning

confidence: 99%

Transcription of AAT•ATT Triplet Repeats in Escherichia coli Is Silenced by H-NS and IS1E Transposition

et al. 2010

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BackgroundThe trinucleotide repeats AAT•ATT are simple DNA sequences that potentially form different types of non-B DNA secondary structures and cause genomic instabilities in vivo.Methodology and Principal FindingsThe molecular mechanism underlying the maintenance of a 24-triplet AAT•ATT repeat was examined in E.coli by cloning the repeats into the EcoRI site in plasmid pUC18 and into the attB site on the E.coli genome. Either the AAT or the ATT strand acted as lagging strand template in a replication fork. Propagations of the repeats in either orientation on plasmids did not affect colony morphology when triplet repeat transcription using the lacZ promoter was repressed either by supplementing LacIQ in trans or by adding glucose into the medium. In contrast, transparent colonies were formed by inducing transcription of the repeats, suggesting that transcription of AAT•ATT repeats was toxic to cell growth. Meanwhile, significant IS1E transposition events were observed both into the triplet repeats region proximal to the promoter side, the promoter region of the lacZ gene, and into the AAT•ATT region itself. Transposition reversed the transparent colony phenotype back into healthy, convex colonies. In contrast, transcription of an 8-triplet AAT•ATT repeat in either orientation on plasmids did not produce significant changes in cell morphology and did not promote IS1E transposition events. We further found that a role of IS1E transposition into plasmids was to inhibit transcription through the repeats, which was influenced by the presence of the H-NS protein, but not of its paralogue StpA.Conclusions and SignificanceOur findings thus suggest that the longer AAT•ATT triplet repeats in E.coli become vulnerable after transcription. H-NS and its facilitated IS1E transposition can silence long triplet repeats transcription and preserve cell growth and survival.

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“…For example, long repeating tracts of CTG· CAG, CCTG· CAGG, and GAA· TTC are associated with the etiology of myotonic dystrophy type 1 (DM1), type 2(DM2), and Friedreich's ataxia (FRDA) (Wells, 2007). The repeating sequences involved have potentials to adopt a variety of non-B DNA secondary structures (McMurray, 1999;Pan, 2004Pan, , 2006Pan, , 2009. Studies in various model systems, including Escherichia coli and mammalian cell lines, such as COS-7, CV-1, and HEK-293, have revealed that conditions promoting formation of non-B DNA structures enhanced the repeats instabilities.…”

Section: Modulation Of Supercoiling and Promoting Transcriptionmentioning

confidence: 99%

The Gratuitous Repair on Undamaged DNA Misfold

Pan¹,

Xiao²,

Hong-qun³

et al. 2011

DNA Repair

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The roles of SbcCD and RNaseE in the transcription of GAA·TTC repeats in Escherichia coli

Cited by 4 publications

References 37 publications

Screening and Structural Characterization of Heat Shock Response Elements (HSEs) in Entamoeba histolytica Promoters

Screening and Structural Characterization of Heat Shock Response Elements (HSEs) in Entamoeba histolytica Promoters

Transcription of AAT•ATT Triplet Repeats in Escherichia coli Is Silenced by H-NS and IS1E Transposition

The Gratuitous Repair on Undamaged DNA Misfold

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