The Roles of Serum Leptin Concentration and Polymorphism in Leptin Receptor Gene at Codon 109 in Breast Cancer

Abstract

Aims: We investigated the relationship between serum leptin concentrations and polymorphism of the leptin receptor gene and breast cancer. Methods: Serum leptin concentrations were measured by enzyme-linked immunosorbent assay in 47 women with invasive breast cancer compared with 41 age-matched controls without cancer. Genomic DNA was extracted from peripheral blood leukocytes. Genotyping of the leptin receptor gene at codon 109 (LEPR-109) was performed by polymerase chain reaction-restriction fragment length polymorphism. Results: Patients with breast cancer had a higher mean serum leptin concentration than women in the control group, but the difference was not statistically significant. Among those with breast cancer, the serum leptin concentration was higher in women with high-grade cancers (p = 0.020). The LEPR-109RR genotype was more frequent in premenopausal patients with tumors larger than 2 cm (p = 0.039) and in premenopausal women who were overweight (p = 0.029). Among patients with the LEPR-109RR genotype, higher mean serum leptin concentrations were present in those with triple-negative cancers (p = 0.048). Conclusions: Our study suggests an association between serum leptin concentration and tumor progression. LEPR-109 polymorphism in premenopausal women appears to be associated with obesity and tumor progression.

Circulating levels of leptin, adiposity and breast cancer risk

“…The reasons for this observation are unclear and may reflect the play of chance. Although leptin has been shown to be correlated with breast cancer risk in this study and earlier reports (Tessitore et al, 2004;Han et al, 2005;Chen et al, 2006b;Hou et al, 2007;Liu et al, 2007), contradictory results were also documented by other investigators. Some reports suggested a negative correlation between leptin and breast cancer in the premenopausal but not postmenopausal group (Petridou et al, 2000), whereas several authors described that there was no significant association between circulating leptin and breast cancer risk (Mantzoros et al, 1999;Coskun et al, 2003;Sauter et al, 2004;Stattin et al, 2004;Woo et al, 2006).…”

Association of Leptin Receptor Lys109Arg and Gln223Arg Polymorphisms with Increased Risk of Clear Cell Renal Cell Carcinoma

“…Among LEPR SNPs, Lys109Arg and Gln223Arg variants are associated with amino acid substitutions in the extracellular region of the leptin receptor and have potential functional consequences. Reports have been suggested the associations of Lys109Arg and Gln223Arg polymorphisms with an increased risk for several cancers (Liu et al, 2007;Han et al, 2008;Yapijakis et al, 2009;Kim et al, 2012). However, to date, few studies have addressed the relationship between LEPR polymorphisms and CC-RCC risk.…”

“…The frequencies of LEPR polymorphisms also were different in previous reports. The frequencies of Lys109Arg wild-type (A) were 31.52%, 35.2%, 18.09% and Gln223Arg were 51.94%, 58.6%, 8.9% in whites and African American (Nyante et al, 2011), Caucasian (Teras et al, 2009) and Asian (Woo et al, 2006;Liu et al, 2007) normal population, respectively. In our study, the frequencies of Lys109Arg and Gln223Arg wild-types were 20.5% and 15.22% in controls, which was similar to Asian population.…”

Epidemiological determinants of age for effective cervical and breast cancer screening in menopausal women

“…Odds ratios are used to compare the relative odds of the occurrence of the outcome of disease. 7 The odds ratio can also be used to determine whether a particular exposure is a risk factor for a particular outcome and to compare the magnitude of various risk factors for that outcome. The 95% confidence interval is used to estimate the precision of the odds ratio.…”