The roles of the α1-adrenergic receptor subtypes in rat embryonic implantation

Ducza, Eszter; Gáspár, Róbert; Mihályi, Attila; Kormányos, Zsolt; Falkay, George

doi:10.1016/j.fertnstert.2008.01.081

Cited by 5 publications

(6 citation statements)

References 18 publications

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“…This is corroborated by the findings that the highest expressions of Kiss1r were found in non-pregnant and 5-day pregnant samples. The contractility of the non-pregnant uterus is usually low in rats (Ducza et al, 2009), while the 5 th gestational day is considered as the time of embryo implantation in rats (Garcia et al, 2017), which requires relative quiescence in the uterine activity. Surprisingly, KISS1 94-121 exerted a more potent uterorelaxant effect in the non-pregnant and 20-day pregnant uteri without the endometrial layer.…”

Section: Discussionmentioning

confidence: 99%

The ontogeny of kisspeptin receptor in the uterine contractions in rats: Its possible role in the quiescence of non-pregnant and pregnant uteri

Schaffer

Hajagos-Tóth

Ducza

et al. 2021

European Journal of Pharmacology

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The objectives of this study were to investigate the effects of KISS1 94-121 fragment on the contractility of nonpregnant and pregnant rat uteri, and to determine the uterine and myometrial expressions of Kiss1r. Uterine muscle strips were obtained from non-pregnant Sprague-Dawley rats in oestrous phase and from pregnant rats on gestational days 5, 15, 18, 20 or 22. The in vitro contractility measurements were carried out in an isolated organ bath in the presence of KISS1 94-121. Experiments with 5-day pregnant tissues were also performed in the presence of kisspeptin-234 trifluoroacetate. The mRNA and protein expressions of Kiss1r were measured by RT-PCR and Western blot analysis, while localizations of receptors were defined by fluorescent immunohistochemistry. KISS1 94-121 induced a dose-dependent relaxation both in non-pregnant and pregnant intact and endometrium-denuded uteri. A gradual decrease was found in the uterine expressions of Kiss1r mRNA and protein towards the end of the gestational period, and it was further confirmed by the immunohistochemical results. The significant majority of Kiss1r is found in the myometrium, however the few endometrial Kiss1r also influences the uterine contractions. The relaxing effect of kisspeptin is continuously reduced towards the end of gestational period in parallel with the reduction of Kiss1r expression. Our results suggest a putative role of kisspeptin in the maintenance of uterine quiescence that may have significance in miscarriage or preterm contractions.

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Section: Discussionmentioning

confidence: 99%

The ontogeny of kisspeptin receptor in the uterine contractions in rats: Its possible role in the quiescence of non-pregnant and pregnant uteri

Schaffer

Hajagos-Tóth

Ducza

et al. 2021

European Journal of Pharmacology

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“…Using above substances, we also found that α1-ARs subtype contributes to an increase in tension in the SAL group and the maintenance of this parameter in the E. coli group. Earlier in relation to the isoforms of α1-ARs, crucial roles of α1A-ARs in embryonic implantation [8] and of α1A-and α1D-ARs in course of late pregnancy [6,17], as well as importance of α1B-ARs for the onset of parturition [5] in rats were reported. Application of antagonists of particular isoforms of α1-ARs (present experiment) showed that in healthy uteri, NA action increasing the amplitude and frequency is mediated by α1A-and α1D-ARs.…”

Section: Discussionmentioning

confidence: 99%

“…In pig uterus, the most numerous are sympathetic fibers which are found in all layers of the uterus [3,4]. Under physiological conditions, expression of all isoforms of α1-and α2-ARs, and all subtypes of β-ARs, was revealed in myocytes of myometrium (MYO) in animals [5][6][7][8][9][10][11][12][13]. Activation of α-ARs by NA stimulates, while activation of β-ARs decreases, myometrial contractility [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

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Effect of blocking of alpha1-adrenoreceptor isoforms on the noradrenaline-induced changes in contractility of inflamed pig uterus

Jana

Całka

2023

PLoS ONE

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Background Disturbances in uterine contractility often lead to the origin, development and maintenance of endometritis and metritis, which are a very common and serious pathologies in domestic animals. Here we aimed to investigate the role of α1A-, α1B- and α1D-adrenoreceptors (ARs) in noradrenaline (NA)-induced contractility of inflammatory-changed porcine uterus. Methods On Day 3 of the estrous cycle, either Escherichia coli (E. coli) suspension (E. coli group) or saline (SAL group) was injected into uterine horns, or only laparotomy was performed (CON group). Eight days later, infected gilts developed severe acute endometritis. Results Compared to the period before NA application, NA reduced the contractile amplitude and frequency in myometrium (MYO) and endometrium (ENDO)/MYO strips from the CON, SAL and E. coli groups. In the last group, the amplitude in MYO and the frequency in ENDO/MYO were lowered versus other groups. After using α1A-ARs antagonist with NA, a greater decrease or occurrence of a drop in the amplitude and frequency in all groups (ENDO/MYO) were found compared to this neurotransmitter action alone. Such results were noted for NA action on the frequency after α1B-ARs blocking in the CON (both kinds of strips) and SAL (ENDO/MYO) groups. In response to α1D-ARs antagonist with NA, a greater decrease or occurrence of a drop in the amplitude was noted in the CON (both kinds of strips) and SAL and E. coli (MYO) groups. Use of these factors caused the similar changes in the frequency in CON and E. coli (MYO) and SAL (ENDO/MYO) groups. In response to NA, α1A,B,D-ARs antagonist led to a greater reduction or appearance of a drop in the amplitude in the CON and SAL (ENDO/MYO) and E. coli (both kinds of strips) as well as in the frequency in the CON and SAL (ENDO/MYO) and E. coli (MYO) groups. Conclusions In conclusion, activation of α1A- and α1D-ARs by NA promotes the contractile amplitude and frequency in the inflamed pig uterus; pharmacological modulation of these receptors can be utilized to enhance systolic activity of myometrium.

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“…The α 1 -AR agonists elicit contractions in the myometrium (Ducza et al 2002, Ducza et al 2005). All of the α 1 -AR subtypes are found in the early-pregnant rat uterus, with a predominance of the α 1A -ARs, while the α 1B -ARs were not detected in the late-pregnant (day 18-22) rat uterus (Ducza et al 2009). The mRNA expression of the α 1A -ARs was highest on day 22 of pregnancy, which suggests that they are involved in the increase in uterine contractility at the end of gestation (Ducza et al 2002).…”

Section: The α 1 -Adrenergic Receptorsmentioning

confidence: 93%

The effects of female sexual hormones on α1- and α2- adrenergic receptor subtypes in the pregnant rat myometrium

Bóta¹

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The roles of the α1-adrenergic receptor subtypes in rat embryonic implantation

Abstract: We assume that the alpha(1A)-AR predominance plays a crucial role in embryonic implantation in the rat.

Cited by 5 publications

References 18 publications

The ontogeny of kisspeptin receptor in the uterine contractions in rats: Its possible role in the quiescence of non-pregnant and pregnant uteri

The ontogeny of kisspeptin receptor in the uterine contractions in rats: Its possible role in the quiescence of non-pregnant and pregnant uteri

Effect of blocking of alpha1-adrenoreceptor isoforms on the noradrenaline-induced changes in contractility of inflamed pig uterus

The effects of female sexual hormones on α1- and α2- adrenergic receptor subtypes in the pregnant rat myometrium

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