The Rose Bengal test

Davies, Graham

doi:10.1136/vr.88.17.447

Cited by 53 publications

(29 citation statements)

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“…The differences in sero-prevalence observed from the previous researchers, might be due to differences in herd size, camel origin, tests used, management conditions, and the presence or absence of infectious foci, such as Brucellainfected herds, which could spread the disease among contact herds. The RBPT detected 93 (11.6%) reactors lower than BAPAT which detects 103 (12.9%) reactors, this variation on the incidence of positive reactors may be attributed to the difference in the acidity of their antigen as reported by Davis (1971) and Corbel (1973). The acidic pH of the RBPT antigen (3.65±0.05) inhibits more amount of IgM fraction (Alton et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

Sero-prevalence of camel brucellosis (Camelus dromedarius) and phenotypic characteristics of Brucella melitensis biovar 3 in Shalateen City, Red Sea Governorate, Egypt

El‐Sayed¹,

Sayed-Ahmed²,

El-Diasty³

et al. 2017

Afr. J. Microbiol. Res.

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The objective of this study was to estimate the sero-prevalence of brucellosis in camels in Shalateen city, Red sea Governorate. A total of 801 Sera were collected from apparently healthy dromedary camels from 2014 to 2015 spring. Sera were consequently serologically tested and confirmed using Rose Bengal plate test (RBPT), buffer acidified plate antigen test (BAPAT) and complement fixation test (CFT). 103 (12.90%), 93 (11.60%), and 92 (11.50%) were positive for RBPT, BABAT and CFT, respectively. Young camels were more sero-positive than old one (13.30 vs.10.80%). In addition, females were more sero-positive than males (19.10 vs. 7.10%). Moreover, Brucella melitensis biovar 3 was isolated from stomach content of aborted camel fetus. Statistically, the apparent prevalence (AP) was estimated to be 11.50%, while true prevalence (TP) was 13.60% (95% CI: 11.20 to 16%; P < 0.05). There was non statistical significant association between different age groups, while a highly significant difference were detected between seasons and genders. This study documented a high prevalence of camel brucellosis in the area of study and there is a need for planning and implementation of joint programs by stakeholders in prevention and control of the disease as well as raising public awareness in decreasing the distribution of the disease.

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Section: Discussionmentioning

confidence: 99%

Sero-prevalence of camel brucellosis (Camelus dromedarius) and phenotypic characteristics of Brucella melitensis biovar 3 in Shalateen City, Red Sea Governorate, Egypt

El‐Sayed¹,

Sayed-Ahmed²,

El-Diasty³

et al. 2017

Afr. J. Microbiol. Res.

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“…Thus, in surveys of large numbers of sera conducted at this laboratory, Morgan et al (1969) and Davies (1971) found a high degree of consistency between the results of the CF test and those of the RBPT. In some cases a positive RBPT reaction was observed in the absence of detectable CF antibody but the reverse situation was rarely encountered.…”

Section: Discussionmentioning

confidence: 75%

“…Comparison of the results of the combined CF and SA tests, interpreted as laid down for the Brucellosis (Accredited Herds) Scheme, with those of the Rose Bengal plate test (RBPT) showed agreement for 90-8 % of sera. Later results (Davies, 1971) showed agreement for 97 % of sera. At present the RBPT is in routine use for screening field samples of cattle sera in connexion with the Brucellosis Incentives Scheme.…”

Section: Introductionmentioning

confidence: 78%

Identification of the immunoglobulin class active in the Rose Bengal plate test for bovine brucellosis

Corbel

1972

Epidemiol. Infect.

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SUMMARYThe antibodies active in the Rose Bengal plate test (RBPT) for bovine brucellosis have been studied. The results of fractionation experiments showed that RBPT activity was associated with fractions containing immunoglobulin of the IgGQ class; other immunoglobulin classes were inactive in this respect although active in other tests. These results were confirmed by inhibition tests with specific antisera and by elution of the antibody from agglutinated RBPT antigen.The major proportion of the serum complement-fixing activity was also present in the IgG, fraction and it is suggested that the RBPT and CF reactions are probably mediated by the same antibodies.

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“…1975;Chappel et al 1978;Morgan, MacKinnon & Cullen, 1969;Morgan & Richards, 1974;Nicoletti, 1967;Rose & Roepke, 1957). Most cattle infected with B. abortus, except those which are recently infected, can be identified by the SAT test (Davies, 1971). However, it has been reported that other supplemental serological methods were superior to SAT for detecting Brucella antibodies in sera from culture positive cows (Nicoletti, 1969).…”

Section: Introductionmentioning

confidence: 99%

Comparison of serological methods for the detection of B. abortus antibodies in sera from vaccinated and non-vaccinated cattle

Heck

Williams

Crawford

et al. 1979

J. Hyg.

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SUMMARYA total of 4551 sera from 863 Strain 19 vaccinated and non-vaccinated adult cattle, independent of disease status, were tested by five serological methods to detect the presence of antibodies to B. abortus. Results from Standard Agglutination Tube (SAT), Buffered Brucella Antigen or card (CT), Complement Fixation (CF), Enzyme Linked Immunosorbent Assay (ELISA) and Rivanol (Riv) methods were compared.There was a 95 % probability for agreement among CT negative sera, between serological methods, for all groups of vaccinated and non-vaccinated cattle. The agreement between tests with Riv Positive sera, excluding the calfhood and adult vaccinated group tested by the CF method, was 91-100 %. The probability of a serum which was serologically negative by other methods being Riv negative was 98 %. The usefulness of serological results from Riv (> 1/50) tests for classifying the reactor status of cattle are of doubtful supplemental value to confirm card test positive results.Vaccination history is an important consideration when evaluating serological data on cattle sera particularly from SAT and CF methods.

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The Rose Bengal test

Cited by 53 publications

References 0 publications

Sero-prevalence of camel brucellosis (Camelus dromedarius) and phenotypic characteristics of Brucella melitensis biovar 3 in Shalateen City, Red Sea Governorate, Egypt

Sero-prevalence of camel brucellosis (Camelus dromedarius) and phenotypic characteristics of Brucella melitensis biovar 3 in Shalateen City, Red Sea Governorate, Egypt

Identification of the immunoglobulin class active in the Rose Bengal plate test for bovine brucellosis

Comparison of serological methods for the detection of B. abortus antibodies in sera from vaccinated and non-vaccinated cattle

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