The rostro-caudal position of cardiac myocytes affect their fate

Patwardhan, Vidya; Fernandez, Sonalis; Montgomery, Michael T.; Litvin, Judith

doi:10.1002/(sici)1097-0177(200005)218:1<123::aid-dvdy11>3.0.co;2-6

Cited by 21 publications

(19 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using different techniques, several investigators established that cardiac AP fates are specified between HH4-7 and determined around HH7-8 (Orts Llorca and Collado, 1967;Satin et al, 1988;Inagaki et al, 1993;Yutzey et al, 1995;Patwardhan et al, 2000). We extend these findings by showing, through reciprocal manipulations of RA signalling, that cardiac AP fates remain plastic from HH4-7, but not after HH8.…”

Section: Gata4 As a Marker For The Early Cardiac Fieldsupporting

confidence: 66%

“…However, the putative contribution of this early RA signalling to cardiac AP patterning needs to be evaluated in the light of previous work indicating that cardiac cells commit irreversibly to their AP phenotypes between stages HH7 and 8, but not earlier (Orts-Llorca and Collado, 1967;Satin et al, 1988;Inagaki et al, 1993;Yutzey et al, 1995;Patwardhan et al, 2000) (for a review, see Xavier-Neto et al, 2001). Therefore, although earlier RA signalling by enzymes other than RALDH2 may play a major role in cardiac development and be necessary or permissive for induction of RALDH2 in the appropriate regions of the cardiogenic plate, the data available indicate that the crucial decision between anterior or posterior fates occurs at developmental times when RALDH2 is the only RALDH enzyme expressed in a clear AP pattern in the cardiac mesoderm.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A caudorostral wave of RALDH2 conveys anteroposterior information to the cardiac field

Hochgreb¹,

Linhares²,

Menezes³

et al. 2003

Development

189

192

View full text Add to dashboard Cite

Section: Gata4 As a Marker For The Early Cardiac Fieldsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

A caudorostral wave of RALDH2 conveys anteroposterior information to the cardiac field

Hochgreb¹,

Linhares²,

Menezes³

et al. 2003

Development

189

192

View full text Add to dashboard Cite

“…Chicken and mouse embryo studies indicated that retinoid signaling regulates the fate specification of inflow and out-flow track tissues [17][18][19][20][21][22]. Therefore, we hypothesized that the activation or inactivation of retinoid signaling directs the atrial vs. ventricular fate specification of differentiated hESC cardiac progenitors, and such a mechanism could be used to efficiently generate either hESC-derived atrial-or ventricular-like myocytes.…”

Section: Alternative Retinoid Signals Direct the Differentiation Of Hmentioning

confidence: 99%

“…RA signaling also regulates anterior-posterior polarisation of the heart [17]. Chicken transplantation studies have revealed that the cardiogenic mesoderm from HH stages 4-6, originally fated to be atria, is competent to develop into functional ventricles, and vice versa [18,19]. RA treatment of HH stage 4 cardiogenic tissue activates the expression of the atrium-specific gene AMHC1 in anterior progenitors fated to develop into out-flow track tissues [20].…”

Section: Introductionmentioning

confidence: 99%

Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals

et al. 2010

View full text Add to dashboard Cite

Although myocyte cell transplantation studies have suggested a promising therapeutic potential for myocardial infarction, a major obstacle to the development of clinical therapies for myocardial repair is the difficulties associated with obtaining relatively homogeneous ventricular myocytes for transplantation. Human embryonic stem cells (hESCs) are a promising source of cardiomyocytes. Here we report that retinoid signaling regulates the fate specification of atrial versus ventricular myocytes during cardiac differentiation of hESCs. We found that both Noggin and the panretinoic acid receptor antagonist BMS-189453 (RAi) significantly increased the cardiac differentiation efficiency of hESCs. To investigate retinoid functions, we compared Noggin+RAi-treated cultures with Noggin+RA-treated cultures. Our results showed that the expression levels of the ventricular-specific gene IRX-4 were radically elevated in Noggin+RAi-treated cultures. MLC-2V, another ventricular-specific marker, was expressed in the majority of the cardiomyocytes in Noggin+RAi-treated cultures, but not in the cardiomyocytes of Noggin+RA-treated cultures. Flow cytometry analysis and electrophysiological studies indicated that with 64.7 ± 0.88% (mean ± s.e.m) cardiac differentiation efficiency, 83% of the cardiomyocytes in Noggin+RAi-treated cultures had embryonic ventricular-like action potentials (APs). With 50.7 ± 1.76% cardiac differentiation efficiency, 94% of the cardiomyocytes in Noggin+RA-treated cultures had embryonic atrial-like APs. These results were further confirmed by imaging studies that assessed the patterns and properties of the Ca 2+ sparks of the cardiomyocytes from the two cultures. These findings demonstrate that retinoid signaling specifies the atrial versus ventricular differentiation of hESCs. This study also shows that relatively homogeneous embryonic atrial-and ventricular-like myocyte populations can be efficiently derived from hESCs by specifically regulating Noggin and retinoid signals.

show abstract

“…AMHC1) and posterior region expressing ventricular marker (i.e. VMHC1) [284]. Inhibition of RA signaling in specific time interval during mouse and chicken development (i.e.…”

Section: Signaling Pathways Underlying Cardiac Differentiationmentioning

confidence: 99%

Differentiation of Human Atrial Myocytes From Endothelial Progenitor Cell-Derived Induced Pluripotent Stem Cells

Jambi¹,

Ye²,

Gollob³

et al. 2013

Canadian Journal of Cardiology

View full text Add to dashboard Cite

The rostro-caudal position of cardiac myocytes affect their fate

Cited by 21 publications

References 28 publications

A caudorostral wave of RALDH2 conveys anteroposterior information to the cardiac field

A caudorostral wave of RALDH2 conveys anteroposterior information to the cardiac field

Direct differentiation of atrial and ventricular myocytes from human embryonic stem cells by alternating retinoid signals

Differentiation of Human Atrial Myocytes From Endothelial Progenitor Cell-Derived Induced Pluripotent Stem Cells

Contact Info

Product

Resources

About