The routine estimation of haemoglobin as oxyhaemoglobin

Bell, G. H.; Chambers, J. W.; Waddell, M. B. R.

doi:10.1042/bj0390060

Cited by 20 publications

(5 citation statements)

References 4 publications

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“…radius, correcting for trapped plasma according to the data of Chaplin and Mollison (1952). Blood haemoglobin was determined as oxyhaemoglobin (Bell, Chambers, and Waddell, 1945). Total circulating erythrocyte volume was measured by labelling some of the subject's own blood cells with 20 Ac.…”

Section: Subjects and Analytical Methodsmentioning

confidence: 99%

Effects of Posture on Plasma Volume and Some Blood Constituents

1960

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When the posture was changed from horizontal to vertical, or the reverse, the alteration in plasma volume and in the levels of haematocrit, haemoglobin, and plasma protein was much greater in patients with oedema or low plasma protein or albumin concentrations (“the pathological group”) than in patients without these abnormalities (“the control group”). This larger fluid shift, and the larger concentration changes dependent on it, are explained in terms of Starling's hypothesis.In the control group there was scarcely any change in the total mass of intravascular plasma protein with change in posture, but in the pathological group this was often considerable. This was interpreted as indicating increased permeability of the capillaries to protein.Attention is drawn to the influence of uncontrolled alterations in posture on biochemical and haematological measurements and a recommendation is made in order to standardize conditions before taking blood for the analysis of non-diffusible constituents.

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Section: Subjects and Analytical Methodsmentioning

confidence: 99%

Effects of Posture on Plasma Volume and Some Blood Constituents

1960

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“…The lambs were bled from the jugular vein at 3-or 4-day intervals for 8 weeks after inoculation to provide heparinized whole blood and serum for biochemical determinations. The heparinized whole blood was used to measure packed cell volume by microhaematocrit, and haemoglobin by the method of Bell, Chambers & Waddell (1945). Plasma alkaline phosphatase EC 3 .…”

Section: Specimens and Recordsmentioning

confidence: 99%

Pathogenicity of the coccidium Eimeria crandallis in laboratory lambs

Catchpole

Gregory

1985

Parasitology

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Ten colostrum-deprived, coccidia-free lambs were reared artificially in indoor cages. At 4 weeks of age they were allocated to 5 groups of 2: Groups 1-3 received 10(4), 10(5) and 10(6) sporulated oocysts of Eimeria crandallis/lamb respectively, each as a single inoculum. Group 4 lambs received daily doses increasing over a 4-week period. Groups 1-3 showed diarrhoea and weight loss from about day 14, followed by slow recovery from about day 30. The diarrhoea was accompanied by a wave of oocyst output and a reduction in serum albumin and plasma alkaline phosphatase activity. One lamb in group 4 died of an unrelated condition on day 15; the other showed no clinical effect of the oocyst inoculations and its oocyst output was related only to the first week or two of inoculation. Lambs in Group 1 showed no clinical effect or oocyst output following challenge with 10(6) oocysts on day 28. E. crandallis showed high pathogenicity and immunogenicity. Its pathogenicity was not closely related to infecting dose within the range 10(4)-10(6).

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“…Haemoglobin was estimated by the oxyhaemoglobin method of Bell, Chambers, and Waddell (1945), using a photoelectric colorimeter. To test the accuracy of our technique five consecutive readings were made from the same blood sample..…”

Section: Methodsmentioning

confidence: 99%

Anaemia of Rheumatic Fever

Cochran¹

1951

BMJ

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The routine estimation of haemoglobin as oxyhaemoglobin

Cited by 20 publications

References 4 publications

Effects of Posture on Plasma Volume and Some Blood Constituents

Effects of Posture on Plasma Volume and Some Blood Constituents

Pathogenicity of the coccidium Eimeria crandallis in laboratory lambs

Anaemia of Rheumatic Fever

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