The rs4285184 polymorphism of the MGAT1 gene as a risk factor for obesity in the Mexican population

Tapia-Rivera, José C.; Baltazar-Rodríguez, Luz M.; Cárdenas-Rojas, Martha I.; Álvarez, Alan; Bustos-Saldaña, Rafael; Delgado‐Enciso, Iván; Valdez-Velázquez, Laura Leticia; Guzmán-Esquivel, José; Ramírez-Flores, Mario

doi:10.1016/j.medcle.2017.02.011

Cited by 1 publication

(1 citation statement)

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“…Obesity is considered a risk factor for different comorbidities and has been related with high morbidity and mortality [13][14][15]. It has been shown that obesity is a state of chronic inflammation characterized by macrophage infiltration and that the gene expression of molecules related with proinflammatory processes plays an important role during development of the disease [16].…”

Section: Introductionmentioning

confidence: 99%

NLRP3, IL-1β, and Caspase-1 Gene Transcript Identification and Expression by QCM-D in Obese Children

et al. 2019

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Background. Obesity in children is highly prevalent in Mexican population. Adipose tissue has been related to specific pro- and anti-inflammatory cytokine and inflammasome gene and protein expression patterns. Actually, there is no existing biosensor for detecting gene expression patterns in children with obesity. The quartz crystal microbalance with dissipation monitoring (QCM-D) has been used as a transducer for DNA biosensor design. Results. In this study, the gene expression pattern of IL-1β, NLRP3, and CASPASE-1 in children with obesity was successfully determined by means of QCM-D. Gene expression patterns were validated with those obtained by quantitative polymerase chain reaction (qPCR), a validated molecular biology technique for gene expression quantification. QCM-D analysis of the detected mass corresponding results for each of the genes showed a major detected mass for IL-1β, followed by similar NLRP3 and constitutive gene 18S deposited mass and a smaller deposited mass for CASPASE-1. Surprisingly, when comparing mRNA gene expression results for NLRP3, IL-1β, and CASPASE-1 obtained with qPCR and QCM-D, similar patterns were found, revealing greatest expression of IL-1β, followed by NLRP3, with CASPASE-1 being the molecule of least expression in the group of children with obesity. AFM images illustrate the step-by-step changes that took place on the quartz surface. Conclusions. QCM-D proved successfully for determining the gene transcripts and expression of NLRP3, IL-1β, and CASPASE-1 in children with obesity, with similar results validated by qPCR. “QCM-D decreases detection costs compared with a validated molecular biology technique.” The QCM-D biosensor developed by our group was successful for gene expression determination; in the future, it can be used for molecular diagnosis.

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Section: Introductionmentioning

confidence: 99%