The Rsp5 Ubiquitin Ligase Binds to and Ubiquitinates Members of the Yeast CIN85-Endophilin Complex, Sla1-Rvs167

Stamenova, Svetoslava D.; Dunn, Rebecca; Adler, Adam S.; Hicke, Linda A

doi:10.1074/jbc.m313479200

Cited by 76 publications

(88 citation statements)

References 66 publications

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“…Tubulation is then suggested to facilitate the scission process. The yeast amphiphysins have been shown to bind to a number of known endocytic proteins, including Las17p, Sla1p and actin itself (Lombardi and Riezman, 2001;Friesen et al, 2003;Madania et al, 1999;Stamenova et al, 2004). The proteins arrive after actin, remain immobile for a few seconds, then show transient movement into the cell.…”

Section: Actin Regulation During Endocytosis In Budding Yeastmentioning

confidence: 99%

Actin regulation in endocytosis

Smythe

Ayscough

2006

Journal of Cell Science

186

148

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Actin in yeast and the mechanism of endocytosisActively growing budding yeast contains three distinct actin structures that are visible by fluorescence microscopy of Rhodamine-phalloidin-stained cells: cortical actin patches, which are now thought to represent sites of endocytosis; actin cables, which are crucial for polarity and for movement of exocytic vesicles; and a contractile actin ring, which plays a role in cytokinesis (Adams and Pringle, 1984).The first evidence linking actin to the endocytic process was published 13 years ago. A genetic screen for yeast mutants exhibiting defective uptake of a fluid-phase marker revealed Increasing evidence from a variety of cell types has highlighted the importance of the actin cytoskeleton during endocytosis. No longer is actin viewed as a passive barrier that must be removed to allow endocytosis to proceed. Rather, actin structures are dynamically organised to assist the remodelling of the cell surface to allow inward movement of vesicles. The majority of our mechanistic insight into the role of actin in endocytosis has come from studies in budding yeast. Although endocytosis in mammalian cells is clearly more complex and subject to a greater array of regulatory signals, recent advances have revealed actin, and actin-regulatory proteins, to be present at endocytic sites. Furthermore, live cell imaging indicates that spatiotemporal aspects of actin recruitment and vesicle formation are likely to be conserved across eukaryotic evolution.

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Section: Actin Regulation During Endocytosis In Budding Yeastmentioning

confidence: 99%

Actin regulation in endocytosis

Smythe

Ayscough

2006

Journal of Cell Science

186

148

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“…Rsp5 could affect endocytosis by interfering with the organization of the actin cytoskeleton. A link between Rsp5 and the actin cytoskeleton is suggested by genetic interactions between rsp5 and several mutants affected in genes encoding actin cytoskeletal components and by the demonstration of direct physical interactions between Rsp5 and components of the actin cytoskeleton [11,12].…”

Section: Introductionmentioning

confidence: 99%

The HECT E3 ubiquitin ligase Rsp5 is important for ubiquitin homeostasis in yeast

Krsmanović

Kölling

2004

FEBS Letters

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The HECT E3 ubiquitin ligase Rsp5, a yeast member of the Nedd4 family, has been implicated in many different aspects of cell physiology. Here, we present evidence that Rsp5 function is important for ubiquitin homeostasis. Several observations suggest that ubiquitin is limiting in the rsp5-1 mutant. Reduced synthesis of ubiquitin appears to contribute to ubiquitin depletion. A transient inhibition of general protein synthesis is observed in a wildtype strain upon heat-shock. While the wildtype cells quickly recover from this transient arrest, the rsp5-1 cells remain arrested. This suggests that Rsp5 is important for recovery from heat-induced protein synthesis arrest. Our results suggest that rsp5 phenotypes should be interpreted with caution, since some of the phenotypes could be simply the result of ubiquitin limitation.

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“…Rsp5p impacts a wide variety of physiological processes, including regulation of endocytosis and lysosomal degradation of plasma membrane permeases such as Fur4p (Galan et al 1996), Gap1p (Springael et al 1999), Tat2p (Beck et al 1999), and receptors such as Ste2p and Ste3p (Dunn and Hicke 2001b). Rsp5p also ubiquitinates a component(s) of the endocytic machinery (Dunn and Hicke 2001a;Gajewska et al 2001;Kamiń ska et al 2002;Stamenova et al 2004). Moreover, Rsp5p-dependent ubiquitination is involved in sorting of amino acid permeases at the Golgi apparatus (Helliwell et al 2001) and in the multivesicular bodies (Katzmann et al 2004;Morvan et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Rsp5 ubiquitin ligase modulates translation accuracy in yeast Saccharomyces cerevisiae

Kwapisz¹,

Cholbinski²,

Hopper³

et al. 2005

RNA

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Rsp5p is an essential yeast ubiquitin protein ligase that ubiquitinates multiple proteins involved in various processes. Recent studies indicate that ubiquitination also affects translation. Here, we show that the strain with the rsp5-13 mutation exhibits altered sensitivity to antibiotics and a slower rate of translation. Using a sensitive dual-gene reporter system, we demonstrate that stop codon readthrough efficiency is decreased in the rsp5-13 mutant, while both +1 and -1 frameshifting were unaffected. The effect of the rsp5-13 mutation on readthrough could be reversed by increased expression of ubiquitin and partially suppressed by overproduction of the elongation factor eEF1A. As assessed by fluorescence in situ hybridization, the rsp5-13 mutant cells accumulate tRNA nuclear pools, perhaps depleting tRNA from the cytoplasm. Nuclear accumulation of tRNA is observed only when rsp5-13 cells are grown in media with high amino acid content. This defect, also reversed by overproduction of the elongation factor eEF1A, may be the primary reason for altered translational decoding accuracy.

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The Rsp5 Ubiquitin Ligase Binds to and Ubiquitinates Members of the Yeast CIN85-Endophilin Complex, Sla1-Rvs167

Cited by 76 publications

References 66 publications

Actin regulation in endocytosis

Actin regulation in endocytosis

The HECT E3 ubiquitin ligase Rsp5 is important for ubiquitin homeostasis in yeast

Rsp5 ubiquitin ligase modulates translation accuracy in yeast Saccharomyces cerevisiae

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