The rumen microbiome inhibits methane formation through dietary choline supplementation

Li, Yang; Kreuzer, Michael; Clayssen, Quentin; Ebert, Marc‐Olivier; Ruscheweyh, Hans‐Joachim; Sunagawa, Shinichi; Kunz, Carmen; Attwood, Graeme T.; Amelchanka, Sergej L.; Terranova, Melissa

doi:10.1038/s41598-021-01031-w

Cited by 11 publications

(6 citation statements)

References 71 publications

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“…Additionally, we found that the genus Dentirobacterium , which occupies a very small proportion of the microbiota, was only observed after red seaweed extract supplementation, with ATAX showing the greatest enrichment ( Table 4 ). Denitrobacterium species metabolize a variety of nitrocompounds in the rumen by oxidizing H 2 or formate ( 77 ) and can consume lactate, which is an intermediate product, and H 2 sink ( 78 ). Anderson et al ( 79 , 80 ) demonstrated that Denitrobacterium detoxificans added to the rumen culture primarily directly inhibit CH 4 formation.…”

Section: Discussionmentioning

confidence: 99%

Red seaweed extracts reduce methane production by altering rumen fermentation and microbial composition in vitro

et al. 2022

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A series of in vitro batch culture incubations were carried out to investigate changes in rumen fermentation characteristics, methane (CH4) production, and microbial composition in response to supplementation with five different red seaweed species (Amphiroa anceps, AANC; Asparagopsis taxiformis, ATAX; Chondracanthus tenellus, CTEN; Grateloupia elliptica, GELL; and Gracilaria parvispora, GPAR). Prior to the incubations, the total flavonoid and polyphenol content of the red seaweed extracts was quantified. The incubated substrate consisted of timothy hay and corn grain [60:40 dry matter (DM) basis]. Treatments were substrate mixtures without seaweed extract (CON) or substrate mixtures supplemented with 0.25 mg/mL of red seaweed extract. Samples were incubated for 6, 12, 24, 36, and 48 h. Each sample was incubated in triplicates in three separate runs. In vitro DM degradability, fermentation parameters (i.e., pH, volatile fatty acids, and ammonia nitrogen), total gas production, and CH4 production were analyzed for all time points. Microbial composition was analyzed using 16S rRNA amplicon sequencing after 24 h of incubation. The highest CH4 reduction (mL/g DM, mL/g digested DM, and % of total gas production) was observed in ATAX (51.3, 50.1, and 51.5%, respectively, compared to CON; P < 0.001) after 12 h of incubation. The other red seaweed extracts reduced the CH4 production (mL/g DM; P < 0.001) in the range of 4.6–35.0% compared to CON after 24 h of incubation. After 24 h of incubation, supplementation with red seaweed extracts tended to increase the molar proportion of propionate (P = 0.057) and decreased the acetate to propionate ratio (P = 0.033) compared to the CON. Abundances of the genus Methanobrevibacter and total methanogens were reduced (P = 0.050 and P = 0.016) by red seaweed extract supplementation. The linear discriminant analysis effect size (P < 0.05, LDA ≥ 2.0) showed that UG Succinivibrionaceae, Anaeroplasma, and UG Ruminococcaceae, which are associated with higher propionate production, starch degradation, and amylase activity were relatively more abundant in red seaweed extracts than in the CON. Our results suggest that supplementation with red seaweed extracts altered the microbiota, leading to the acceleration of propionate production and reduction in CH4 production.

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Section: Discussionmentioning

confidence: 99%

Red seaweed extracts reduce methane production by altering rumen fermentation and microbial composition in vitro

et al. 2022

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“…It is capable of using trimethylamine to produce CH 4 [32]. Most trimethylamines are derived from choline in the rumen, which strongly inhibits CH 4 emission [33]. In the present study, the higher abundance of UG Methanomethylophilaceae in the CON group may be attributed to the lower concentration of trimethylamine, which could potentially be utilized as a source of CH 4 .…”

Section: Discussionmentioning

confidence: 54%

Application of multi-omics to investigate the effect of Pinus koraiensis cone essential oil on rumen methane emission, microbial community, and metabolites

Choi,

Lee,

Kim

et al. 2023

Preprint

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Background Enteric methane (CH4) excreted by ruminants is a major source of anthropogenic greenhouse gas emissions in the global environment. Pinus koraiensis cone essential oil (PEO) contains functional compounds such as monoterpene hydrocarbons, which can directly affect the microbiota and their function in the rumen. Previously, we found that PEO oral administration during the growing phases of goats reduced CH4 emissions and was associated with the rumen prokaryotic microbiota. However, a more comprehensive analysis of the rumen microbiota and metabolites are needed. The objective was to elucidate the potential microbial features that underpin CH4 mitigation in goats using metataxonomics (prokaryotes, protozoa, and fungi) and metabolomics (rumen fluid and serum). Ten fattening Korean native goats were divided into two dietary groups: control (CON; basal diet without additives) and PEO (basal diet + 1.5 g/d of PEO), using a 2 × 2 crossover design for 11 weeks. Methane measurements were conducted every four consecutive days for 24–27 d. Results Oral administration of PEO reduced CH4 concentrations in the exhaled gas from eructation by 12.0–13.6% (P < 0.05). Although the microbiota structure, including prokaryotes, protozoa, and fungi, was not altered after PEO administration, MaAsLin2 analysis revealed that Selenomonas, Christensenellaceae R-7, and Anaerovibrio were enriched in the PEO group (Q < 0.1). Co-occurrence network analysis revealed that the Bacteroidales RF16 group and Anaerovibrio were the keystone genera in the CON and PEO groups, respectively, with fungal genera exclusively found in the PEO group but not identified as keystone taxa. Predicted function analysis using CowPI, CH4 metabolism was enriched in the CON group, whereas metabolism of sulfur (P < 0.001) and propionate (P < 0.1) were enriched in the PEO group. Random forest analysis identified eight ruminal metabolites, including propionate, that were altered after PEO administration, with predictive accuracy ranging from 0.75 to 0.88. Selenomonas was positively correlated with propionate and co-occurred with it. Conclusions The results provide an understanding of how PEO oral administration affects the ruminal microbial community and its functions in the rumen, as well as its linkages with rumen metabolites and host health, ultimately leading to the reduced CH4 emissions.

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“…In a recent study, Li et al 53 reported that choline affects the microbiota and is related to methane emission reduction. However, choline was not associated with methane emissions in our study.…”

Section: Discussionmentioning

confidence: 99%

“…Clemmons et al 16 reported that hypoxanthine in ruminal fluid was greater in low-RFI compared to high-RFI steers, however, an opposite relation was observed in feces in the present study. In a recent study, Li et al 53 reported that choline affects the microbiota and is related to methane emission reduction. However, choline was not associated with methane emissions in our study.…”

Section: Phenotype-associated Metabolomementioning

confidence: 99%

Ruminal and feces metabolites associated with feed efficiency, water intake and methane emission in Nelore bulls

Malheiros,

Correia,

Ceribeli

et al. 2023

Sci Rep

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The objectives of this study were twofold: (1) to identify potential differences in the ruminal and fecal metabolite profiles of Nelore bulls under different nutritional interventions; and (2) to identify metabolites associated with cattle sustainability related-traits. We used different nutritional interventions in the feedlot: conventional (Conv; n = 26), and by-product (ByPr, n = 26). Thirty-eight ruminal fluid and 27 fecal metabolites were significantly different (P < 0.05) between the ByPr and Conv groups. Individual dry matter intake (DMI), residual feed intake (RFI), observed water intake (OWI), predicted water intake (WI), and residual water intake (RWI) phenotypes were lower (P < 0.05) in the Conv group, while the ByPr group exhibited lower methane emission (ME) (P < 0.05). Ruminal fluid dimethylamine was significantly associated (P < 0.05) with DMI, RFI, FE (feed efficiency), OWI and WI. Aspartate was associated (P < 0.05) with DMI, RFI, FE and WI. Fecal C22:1n9 was significantly associated with OWI and RWI (P < 0.05). Fatty acid C14:0 and hypoxanthine were significantly associated with DMI and RFI (P < 0.05). The results demonstrated that different nutritional interventions alter ruminal and fecal metabolites and provided new insights into the relationship of these metabolites with feed efficiency and water intake traits in Nelore bulls.

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The rumen microbiome inhibits methane formation through dietary choline supplementation

Cited by 11 publications

References 71 publications

Red seaweed extracts reduce methane production by altering rumen fermentation and microbial composition in vitro

Red seaweed extracts reduce methane production by altering rumen fermentation and microbial composition in vitro

Application of multi-omics to investigate the effect of Pinus koraiensis cone essential oil on rumen methane emission, microbial community, and metabolites

Ruminal and feces metabolites associated with feed efficiency, water intake and methane emission in Nelore bulls

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