The ruthenium complex cis-(dichloro)tetrammineruthenium(III) chloride induces apoptosis and damages DNA in murine sarcoma 180 cells

Lima, Aliny Pereira de; Pereira, Flávia de Castro; Vilanova-Costa, Cesar Augusto Sam Tiago; Ribeiro, Alessandra de Santana Braga Barbosa; Pavanin, Luiz Alfredo; Santos, Wagner Batista dos; Silveira-Lacerda, Elisângela de Paula

doi:10.1007/s12038-010-0042-2

Cited by 16 publications

(2 citation statements)

References 29 publications

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“…In vitro studies have confirmed the ability of ruthenium complexes to bind to DNA [40]. The cytotoxicity in cell cultures and genotoxicity in in vitro and in vivo models indicate a direct correlation of this capacity [41][42][43].…”

Section: Discussionmentioning

confidence: 82%

In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: a promising class of antituberculosis agents

Grandis

Resende

Silva

et al. 2016

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Tuberculosis is a top infectious disease killer worldwide, caused by the bacteria Mycobacterium tuberculosis. Increasing incidences of multiple drug-resistance (MDR) strains are emerging as one of the major public health threats. However, the drugs in use are still incapable of controlling the appalling upsurge of MDR. In recent years a marked number of research groups have devoted their attention toward the development of specific and cost-effective antimicrobial agents against targeted MDR-Tuberculosis. In previous studies, ruthenium(II) complexes (SCAR) have shown a promising activity against MDR-Tuberculosis although few studies have indeed considered ruthenium toxicity. Therefore, within the preclinical requirements, we have sought to determine the cyto-genotoxicity of three SCAR complexes in this present study. The treatment with the SCARs induced a concentration-dependent decrease in cell viability in CHO-K1 and HepG2 cells. Based on the clonogenic survival, SCAR 5 was found to be more cytotoxic while SCAR 6 exhibited selectivity action on tumor cells. Although SCAR 4 and 5 did not indicate any mutagenic activity as evidenced by the Ames and Cytokinesis block micronucleus cytome assays, the complex SCAR 6 was found to engender a frameshift mutation detected by Salmonella typhimurium in the presence of S9. Similarly, we observed a chromosomal damage in HepG2 cells with significant increases of micronuclei and nucleoplasmic bridges. These data indicate that SCAR 4 and 5 complexes did not show genotoxicity in our models while SCAR 6 was considered mutagenic. This study presented a comprehensive genotoxic evaluation of SCAR complexes were shown to be genotoxic in vitro. All in all, further studies are required to fully elucidate how the properties can affect human health.

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Section: Discussionmentioning

confidence: 82%

In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: a promising class of antituberculosis agents

Grandis

Resende

Silva

et al. 2016

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…Following light activation, complex 1 leads to the disappearance of both bands, suggesting a possible interaction with DNA, causing fragmentation. DNA fragmentation is often observed upon treatment of cancer cells with ruthenium-based drugs [ 62 , 63 , 64 , 65 ]. We have previously shown that irradiation of Ru(II)bipy 2 BC in pUC18 leads to the disappearance of the EtBr signal, suggesting DNA damage and fragmentation [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

A Ru(II)-Strained Complex with 2,9-Diphenyl-1,10-phenanthroline Ligand Induces Selective Photoactivatable Chemotherapeutic Activity on Human Alveolar Carcinoma Cells via Apoptosis

Mansour,

Mehanna,

Bodman-Smith

et al. 2023

Pharmaceuticals

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[Ru(bipy)2(dpphen)]Cl2 (where bipy = 2,2′-bipyridine and dpphen = 2,9-diphenyl-1,10-phenanthroline) (complex 1) is a sterically strained compound that exhibits promising in vitro photocytotoxicity on an array of cell lines. Since lung adenocarcinoma cancer remains the most common lung cancer and the leading cause of cancer deaths, the current study aims to evaluate the plausible effect and uptake of complex 1 on human alveolar carcinoma cells (A549) and mesenchymal stem cells (MSC), and assess its cytotoxicity in vitro while considering its effect on cell morphology, membrane integrity and DNA damage. MSC and A549 cells showed similar rates of complex 1 uptake with a plateau at 12 h. Upon photoactivation, complex 1 exhibited selective, potent anticancer activity against A549 cells with phototoxicity index (PI) values of 16, 25 and 39 at 24, 48 and 72 h, respectively. This effect was accompanied by a significant increase in A549-cell rounding and detachment, loss of membrane integrity and DNA damage. Flow cytometry experiments confirmed that A549 cells undergo apoptosis when treated with complex 1 followed by photoactivation. In conclusion, this present study suggests that complex 1 might be a promising candidate for photochemotherapy with photoproducts that possess selective anticancer effects in vitro. These results are encouraging to probe the potential activity of this complex in vivo.

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Ruthenium (II)/allopurinol complex inhibits breast cancer progression via multiple targets

et al. 2021

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The ruthenium complex cis-(dichloro)tetrammineruthenium(III) chloride induces apoptosis and damages DNA in murine sarcoma 180 cells

Cited by 16 publications

References 29 publications

In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: a promising class of antituberculosis agents

In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: a promising class of antituberculosis agents

A Ru(II)-Strained Complex with 2,9-Diphenyl-1,10-phenanthroline Ligand Induces Selective Photoactivatable Chemotherapeutic Activity on Human Alveolar Carcinoma Cells via Apoptosis

Ruthenium (II)/allopurinol complex inhibits breast cancer progression via multiple targets

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