The S11 and S13 self incompatibility alleles in Solanum chacoense Bitt. are remarkably similar

Saba-El-Leil, Mare K.; Rivard, Sylvain R.; Morse, David; Cappadocia, Mario

doi:10.1007/bf00023555

Cited by 64 publications

(35 citation statements)

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“…Since the pairwise comparisons of the deduced amino-acid sequences detected no completely matching pairs, almost all the Sgenotypes determined by RFLP analysis were expected to reflect the self-incompatibility phenotypes. Two pairs showed high homology, but this does not necessarily mean that their phenotypes were the same, since a small number of amino-acid substitutions appear to be sufficient to trigger the self-incompatibility reaction between the S1/S4 and S3/S5 pairs of Pyrus pyrifolia (Ishimizu et al, 1998), and the S11/S13 pair of Solanum chacoense (Saba-El-Leil et al, 1994). From so few cases it cannot be definitively concluded that a few substitutions are sufficient to express different specificities.…”

Section: Discussionmentioning

confidence: 99%

Distribution of S-alleles in island populations of flowering cherry, Prunus lannesiana var. speciosa

et al. 2007

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We surveyed the distribution of S-alleles in natural island populations of Prunus lannesiana var. speciosa sampled from seven sites on the Izu Peninsula and six Izu islands, Japan. The S-genotypes of sampled individuals were determined by Southern analysis of RFLPs generated by restriction enzyme digestion of genomic DNA, using cDNA of the S-RNase gene as a probe. All individuals were heterozygous, as expected under gametophytic self-incompatibility (GSI). Sixty-three Salleles were observed in the species, but 12 private to the Izu Peninsula population seemed to be derived from related species, giving a total of 75. The estimated number of S-alleles in each population ranged from 26 to 62, and was inversely correlated with the respective population's distance from the Izu Peninsula, the closest point in the mainland to the islands. This geographical cline in the estimated numbers of S-alleles suggests that gene flow to and from the distant island populations was less frequent, and that the studied species has migrated from the mainland to the Izu islands. The genetic relationship at the S-locus among populations also gave an "isolation by distance" pattern. The genetic differentiation at the S-locus among the populations was very low (F ST = 0.014, p < 0.001). The number of S-alleles in the species did not seem to depend on genetic differences associated with population subdivisions. This might be due to the greater effective migration rates of S-alleles, as expected under balancing selection in GSI.

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Section: Discussionmentioning

confidence: 99%

Distribution of S-alleles in island populations of flowering cherry, Prunus lannesiana var. speciosa

et al. 2007

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“…At least one intron is expected, since the S-alleles of SRNases of Solanaceae and Rosaceae usually have a single intron (in the HVa region, from 87 bp to 120 bp, and 138 bp to 1100 bp, respectively, in sequences from these two families; Saba-El- Leil et al, 1994;Matton et al, 1995;Broothaerts et al, 1995). Prunus (Rosaceae) S-RNases have at least two introns (in P. avium, one 5′ to the C1 region, and one in HVa; Tao et al, 1999), and a sequence from P. dulcis (GenBank accession number AF157008) is similar (Figure 1).…”

Section: Analysis Of a Graniticum Crossesmentioning

confidence: 99%

Molecular variation at the self-incompatibility locus in natural populations of the genera Antirrhinum and Misopates

Vieira

Charlesworth

2002

Heredity

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The self-incompatibility system of flowering plants is a classic example of extreme allelic polymorphism maintained by frequency-dependent selection. We used primers designed from three published Antirrhinum hispanicum Sallele sequences in PCR reactions with genomic DNA of plants sampled from natural populations of Antirrhinum and Misopates species. Not surprisingly, given the polymorphism of S-alleles, only a minority of individuals yielded PCR products of the expected size. These yielded 35 genomic sequences, of nine different sequence types of which eight are highly similar to the A. hispanicum S-allele sequences, and one to a very similar unpublished Antirrhinum S-like

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“…Our experimental system, the tuber-bearing wild potato species S. chacoense , involves two phenotypically distinct S alleles ( S 11 and S 13 ) whose mature proteins differ by only 10 amino acids (Saba-El-Leil et al, 1994), four of which are located in the hypervariable regions ( Figure 1B). We have shown that the substitution of all four of these amino acids in the hypervariable regions of an S 11 RNase with those of an S 13 RNase fully converts the S 11 phenotype into an S 13 phenotype (HVab S RNase; Matton et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…In the gametophytic system, several studies (Clark and Kao, 1991;Coleman and Kao, 1992;Saba-El-Leil et al, 1994;Matton et al, 1995Matton et al, , 1997Ishimizu et al, 1998) have suggested that point mutations rather than intragenic recombination (Fisher, 1961;Pandey, 1970;Ebert et al, 1989) are the primary source of S allele polymorphism. Phenotypically distinct yet highly similar S RNase sequences have been described in Solanum chacoense (Saba-El-Leil et al, 1994) and Pyrus pyriflora (Ishimizu et al, 1998). 1 To whom correspondence should be addressed.…”

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Production of an S RNase with Dual Specificity Suggests a Novel Hypothesis for the Generation of New S Alleles

et al. 1999

Self Cite

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Gametophytic self-incompatibility in plants involves rejection of pollen when pistil and pollen share the same allele at the S locus. This locus is highly multiallelic, but the mechanism by which new functional S alleles are generated in nature has not been determined and remains one of the most intriguing conceptual barriers to a full understanding of selfincompatibility. The S 11 and S 13 RNases of Solanum chacoense differ by only 10 amino acids, but they are phenotypically distinct (i.e., they reject either S 11 or S 13 pollen, respectively). These RNases are thus ideally suited for a dissection of the elements involved in recognition specificity. We have previously found that the modification of four amino acid residues in the S 11 RNase to match those in the S 13 RNase was sufficient to completely replace the S 11 phenotype with the S 13 phenotype. We now show that an S 11 RNase in which only three amino acid residues were modified to match those in the S 13 RNase displays the unprecedented property of dual specificity (i.e., the simultaneous rejection of both S 11 and S 13 pollen). Thus, S 12 S 14 plants expressing this hybrid S RNase rejected S 11 , S 12 , S 13 , and S 14 pollen yet allowed S 15 pollen to pass freely. Surprisingly, only a single base pair differs between the dual-specific S allele and a monospecific S 13 allele. Dual-specific S RNases represent a previously unsuspected category of S alleles. We propose that dualspecific alleles play a critical role in establishing novel S alleles, because the plants harboring them could maintain their old recognition phenotype while acquiring a new one. INTRODUCTIONAmong the cell-cell recognition phenomena present in living organisms, self-incompatibility (SI) plays a major evolutionary role because it constitutes an important mechanism for preventing inbreeding. SI is present in hermaphroditic animals such as tunicates (Grosberg, 1988), in fungi (Kronstad and Leong, 1990), and in many Angiosperm families (de Nettancourt, 1977). In the most widespread type of SI, gametophytic SI, the genotype of the haploid pollen determines its own incompatibility phenotype. For the Solanaceae, the gametophytic SI phenotype is specified by a highly multiallelic S locus (de Nettancourt, 1977(de Nettancourt, , 1997 whose only known product is a ribonuclease (S RNase; McClure et al., 1989) expressed in the transmitting tissue of the style (Anderson et al., 1986). Gain-of-function experiments have shown that expression of an S RNase transgene is sufficient to alter the SI phenotype of the pistil but not that of the pollen Murfett et al., 1994;Matton et al., 1997), and thus the identity of the pollen S gene (unknown to date) is likely to be different from that of the S RNase (Kao and McCubbin, 1997). RNase activity, although essential for expression of the SI phenotype , seems not to be involved in the specificity of the cell-cell recognition phenomenon. In closely related S RNases, such specificity has been shown to depend on the amino acid sequence at the two hypervariable ...

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The S11 and S13 self incompatibility alleles in Solanum chacoense Bitt. are remarkably similar

Cited by 64 publications

References 28 publications

Distribution of S-alleles in island populations of flowering cherry, Prunus lannesiana var. speciosa

Distribution of S-alleles in island populations of flowering cherry, Prunus lannesiana var. speciosa

Molecular variation at the self-incompatibility locus in natural populations of the genera Antirrhinum and Misopates

Production of an S RNase with Dual Specificity Suggests a Novel Hypothesis for the Generation of New S Alleles

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