The Saccharomyces cerevisiae CKS1 gene, a homolog of the Schizosaccharomyces pombe suc1+ gene, encodes a subunit of the Cdc28 protein kinase complex.

Hadwiger, Jeffrey A.; Wittenberg, Curt; Mendenhall, Michael J.; Reed, Steven I.

doi:10.1128/mcb.9.5.2034

Cited by 197 publications

(184 citation statements)

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“…Cloning CKS 1 homologs from human cells CKS1 from S. cerevisiae and sucl + from S. pombe are 67% identical at the amino acid level (Hadwiger et al 1989a). By comparison of the amino acid sequences of these proteins, two degenerate oligonucleotides were designed that could be used in the polymerase chain reaction (PCR)(C. Saiki et al 1988)to amplify a region of 198 bp from the S. cerevisiae CKS1 gene and from an intronless version of the S. pombe sucl + gene (obtained from J.…”

Section: Resultsmentioning

confidence: 99%

“…7B, lane 1; Hadwiger et al 1989a). When the Ckshs-Sepharose beads were incubated with extracts from a strain carrying a temperature-sensitive allele of CDC28, cdc28-4 (from which no Cdc28 activity is detected in the in vitro assay; Reed et al 1985;Mendenhall et al 1987), no significant activity was observed against histone H1 and no p40 phosphorylation was detectable.…”

Section: Ckshsl and Ckshs2 Bind To The Cdc2/cdc28 Protein Kinase Frommentioning

confidence: 99%

“…In S. pombe the sucl + gene product has been shown by genetic and biochemical approaches to interact with Cdc2 (Hayles et al 1986a, b;Brizuela et al 1987;Hindley et al 1987;Moreno et al 1989). Likewise, in S. cerevisiae the Cksl protein has been demonstrated to interact with Cdc28 (Hadwiger et al 1989a). A homolog of Cksl/Sucl has been reported to exist in human cells Draetta and Beach 1988;Bailly et al 1989;Riabowol et al 1989).…”

mentioning

confidence: 99%

“…There has been an accumulation of conflicting data based on work with fission yeast, budding yeast and vertebrates (Hayles et al 1986a;Hindley et al 1987;Dunphy et al 1988;Dunphy and Newport 1989;Hadwiger et al 1989a;Moreno et al 1989;Riabowol et al 1989;S.I. Reed, unpubl.).…”

mentioning

confidence: 99%

“…The S. pombe sucl + gene was isolated as a high-copy suppressor of temperature-sensitive mutations in cdc2 (Hayles et al 1986b). CKS1 was isolated from S. cerevisiae by a similar procedure (Hadwiger et al 1989a) and encodes a protein highly related at the amino acid level to the S. pombe sucl + gene product. In S. pombe the sucl + gene product has been shown by genetic and biochemical approaches to interact with Cdc2 (Hayles et al 1986a, b;Brizuela et al 1987;Hindley et al 1987;Moreno et al 1989).…”

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Human cDNAs encoding homologs of the small p34Cdc28/Cdc2-associated protein of Saccharomyces cerevisiae and Schizosaccharomyces pombe.

Richardson¹,

Stueland²,

Thomas³

et al. 1990

Genes Dev.

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181

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The Cksl protein is a component of the Cdc28 protein kinase in the budding yeast Saccharomyces cererisiae. This paper reports the cloning of two homologs of the S. cererisiae CKS1 gene from human cells. These homologs, CKShsl and CKShs2, both encode proteins of 79 amino acids that share considerable homology at the amino acid level with the products of CKS1 from S. cererisiae and sucl + from the fission yeast Schizosaccharomyces pombe. Both human homologs are capable of rescuing a null mutation of the S. cererisiae CKS1 gene when expressed from the S. cererisiae GALl promoter. S. pombe sucl + expressed from the GALl promoter is also capable of rescuing a S. cererisiae cksl null mutation. Ckshsl or Ckshs2 protein linked to Sepharose beads can bind the Cdc28/Cdc2 protein kinase from both S. cererisiae and human cells. The CKShsl and CKShs2 mRNAs are expressed in different patterns through the cell cycle in HeLa cells, which may reflect specialized roles for the encoded proteins.

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Section: Resultsmentioning

confidence: 99%

Section: Ckshsl and Ckshs2 Bind To The Cdc2/cdc28 Protein Kinase Frommentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Human cDNAs encoding homologs of the small p34Cdc28/Cdc2-associated protein of Saccharomyces cerevisiae and Schizosaccharomyces pombe.

Richardson¹,

Stueland²,

Thomas³

et al. 1990

Genes Dev.

Self Cite

181

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ckshs expression is linked to cell proliferation in normal and malignant human lymphoid cells

Urbanowicz‐Kachnowicz

Baghdassarian

Nakache

et al. 1999

Int. J. Cancer

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Cyclin kinase sub‐units (CKS) are known to interact with cyclin‐dependent kinases (CDKs), but their functions are not completely understood and their expression in human tissues is not documented. For analyzing relationships of CKS with cell proliferation and/or with differentiation, we investigated the expression of ckshs1 and ckshs2 in normal and malignant human lymphoid cells. ckshs1 and ckshs2 expression appeared to be related to cell proliferation: (i) mRNAs increased with stimulation of normal peripheral‐blood lymphocytes, and from the G1 to the SG2M phase in elutriated cells; (ii) P9 proteins were also induced by lymphocyte stimulation and were localized in nucleus where phosphorylated forms of CDK1 were also found; (iii) in vitro, the phosphorylated forms of CDK1 and CDK2 were preferentially linked to CKS. Among 45 patients presenting acute or chronic lymphoid malignancy, ckshs1 and ckshs2 mRNAs varied in a similar way and were significantly correlated to cell proliferation (p < 0.0001). When analysis was restricted solely to acute lymphoblastic leukemia (ALL) this correlation was still found and ckshs1 and ckshs2 were significantly more expressed in T‐cell ALL than in B‐cell‐lineage ALL. These results confirm relationships between ckshs expression and cell proliferation, and pose the question of a link with cell differentiation. Int. J. Cancer 82:98–104, 1999. © 1999 Wiley‐Liss, Inc.

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Alterations in the expression of the cell cycle regulatory protein cyclin kinase subunit 1 in colorectal carcinoma

Shapira

Ben‐Izhak

Bishara

et al. 2004

Cancer

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BACKGROUND Low levels of p27Kip1 are associated with high aggressiveness and poor prognosis in various malignancies, including colorectal carcinoma. The authors showed that S phase kinase protein 2 (Skp2), the specific ubiquitin ligase subunit that targets p27Kip1 for degradation, was overexpressed and was inversely related to p27Kip1 levels in patients with colorectal carcinoma. The essential role of cyclin kinase subunit 1 (Cks1) in Skp2‐dependent p27 degradation was recently discovered, but its role in human malignancies is unknown. METHODS Quick‐frozen colorectal tumor samples from 30 patients were separated by electrophoresis on sodium dodecyl sulfate–polyacrylamide gels, transferred to nitrocellulose, and probed with highly specific monoclonal antibodies directed against Cks1, Skp2, and p27Kip1. The expression of Cks1 was also examined by immunohistochemistry using formalin‐fixed, paraffin‐embedded tissue sections from the same patients. RESULTS A strong correlation was found between Cks1 levels and Skp2 expression and loss of tumor differentiation. A significant inverse relation was also observed between levels of Cks1 and p27Kip1 and overall survival. CONCLUSIONS The results of the current study suggest that increased expression of Cks1 may have an important causative role in decreasing levels of p27 in patients with aggressive colorectal carcinoma. Cancer 2004. © 2004 American Cancer Society.

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The Saccharomyces cerevisiae CKS1 gene, a homolog of the Schizosaccharomyces pombe suc1+ gene, encodes a subunit of the Cdc28 protein kinase complex.

Cited by 197 publications

References 37 publications

Human cDNAs encoding homologs of the small p34Cdc28/Cdc2-associated protein of Saccharomyces cerevisiae and Schizosaccharomyces pombe.

Human cDNAs encoding homologs of the small p34Cdc28/Cdc2-associated protein of Saccharomyces cerevisiae and Schizosaccharomyces pombe.

ckshs expression is linked to cell proliferation in normal and malignant human lymphoid cells

Alterations in the expression of the cell cycle regulatory protein cyclin kinase subunit 1 in colorectal carcinoma

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