The Saccharomyces cerevisiae MER3 gene, encoding a novel helicase-like protein, is required for crossover control in meiosis

Nakagawa, Takuro; Ogawa, Hideyuki

doi:10.1093/emboj/18.20.5714

Cited by 134 publications

(151 citation statements)

References 47 publications

Supporting

Mentioning

138

Contrasting

Order By: Relevance

“…The Mer1 protein binds directly to an element found in the regulated introns of target genes and in the absence of MER1 these genes are not spliced (Nandabalan et al 1993;Spingola and Ares 2000). Four direct targets of Mer1 have been identified: MER2, MER3, SPO22, and AMA1 (Engebrecht et al 1991;Nakagawa and Ogawa 1999;Cooper et al 2000;Davis et al 2000;Spingola and Ares 2000). SPO22 and MER3 are both early genes induced by Ume6/Ime1, while MER2 is constitutively transcribed, but unspliced, in vegetative cells (Engebrecht et al 1991;Munding et al 2010).…”

Section: Key Events In the Phases Of Sporulationmentioning

confidence: 99%

Sporulation in the Budding Yeast Saccharomyces cerevisiae

2011

View full text Add to dashboard Cite

In response to nitrogen starvation in the presence of a poor carbon source, diploid cells of the yeast Saccharomyces cerevisiae undergo meiosis and package the haploid nuclei produced in meiosis into spores. The formation of spores requires an unusual cell division event in which daughter cells are formed within the cytoplasm of the mother cell. This process involves the de novo generation of two different cellular structures: novel membrane compartments within the cell cytoplasm that give rise to the spore plasma membrane and an extensive spore wall that protects the spore from environmental insults. This article summarizes what is known about the molecular mechanisms controlling spore assembly with particular attention to how constitutive cellular functions are modified to create novel behaviors during this developmental process. Key regulatory points on the sporulation pathway are also discussed as well as the possible role of sporulation in the natural ecology of S. cerevisiae. TABLE OF CONTENTS Abstract 737Introduction 738

show abstract

Section: Key Events In the Phases Of Sporulationmentioning

confidence: 99%

Sporulation in the Budding Yeast Saccharomyces cerevisiae

2011

View full text Add to dashboard Cite

show abstract

“…Consistent with this view, none of the eight chromosome III disomes was recombinant. In the absence of Mlh1, Mlh3, Msh4, Msh5, Zip1, Zip2, and Mer3, crossing over is reduced twofold to threefold during meiosis, leading to abnormal reductional division (Ross and Roeder, 1994;Sym and Roeder, 1994;Hollingsworth et al, 1995;Hunter and Borts, 1997;Chua and Roeder, 1998;Nakagawa and Ogawa, 1999;Wang et al, 1999). Mlh1, Mlh3, Msh4, and Msh5 are homologues of mismatch repair proteins, and both Mlh1 and Mlh3, like Exo1, are also required for mismatch correction; thus, a close mechanistic correlation between mismatch repair and crossing over is suggested.…”

Section: Exo1 and Crossing Over During Meiosismentioning

confidence: 99%

“…Gene conversion has no significant role in segregation (Roeder, 1997). A mutation in any one of a group of genes (ZIP1, ZIP2, MSH4, MSH5, MLH1, MLH3, and MER3) reduces crossing over (Ross and Roeder, 1994;Sym and Roeder, 1994;Hollingsworth et al, 1995;Hunter and Borts, 1997;Chua and Roeder, 1998;Nakagawa and Ogawa, 1999;Wang et al, 1999). Among these, MSH4, MSH5, MLH1, and MLH3 are homologues of the mismatch repair proteins of E. coli, suggesting a mechanistic relationship between mismatch repair and crossing over .…”

Section: Introductionmentioning

confidence: 99%

Exo1 Roles for Repair of DNA Double-Strand Breaks and Meiotic Crossing Over inSaccharomyces cerevisiae

Tsubouchi

Ogawa

2000

MBoC

204

188

View full text Add to dashboard Cite

The MRE11, RAD50, and XRS2 genes of Saccharomyces cerevisiae are involved in the repair of DNA double-strand breaks (DSBs) produced by ionizing radiation and by radiomimetic chemicals such as methyl methanesulfonate (MMS). In these mutants, single-strand DNA degradation in a 5Ј to 3Ј direction from DSB ends is reduced. Multiple copies of the EXO1 gene, encoding a 5Ј to 3Ј double-strand DNA exonuclease, were found to suppress the high MMS sensitivity of these mutants. The exo1 single mutant shows weak MMS sensitivity. When an exo1 mutation is combined with an mre11 mutation, both repair of MMS-induced damage and processing of DSBs are more severely reduced than in either single mutant, suggesting that Exo1 and Mre11 function independently in DSB processing. During meiosis, transcription of the EXO1 gene is highly induced. In meiotic cells, the exo1 mutation reduces the processing of DSBs and the frequency of crossing over, but not the frequency of gene conversion. These results suggest that Exo1 functions in the processing of DSB ends and in meiotic crossing over.

show abstract

“…In budding yeast, SPO11 functions in the generation of DSBs [23,24]. MSH4, MSH5 and MER3 are required for normal crossover formation [25][26][27][28]. MER3 is a DNA helicase and is important for extension of the DNA heteroduplex in the second end capture (SEC) intermediate [29].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the distributions of the remaining chiasmata in the mutants are not statistically different from predicted Poisson distributions, suggesting that the formation of the remaining crossovers is not sensitive to interference. However, the wild-type chiasma distribution is dramatically different from the Poisson distribution, demonstrating that budding yeast and Arabidopsis both possess two genetically distinct pathways for crossover formation: a major interference-sensitive pathway dependent on MSH4/5 and MER3 proteins and a minor interference-insensitive pathway that is independent of these proteins [26,[37][38][39][40].…”

Section: Introductionmentioning

confidence: 99%

Functional conservation of the meiotic genes SDS and RCK in male meiosis in the monocot rice

2009

View full text Add to dashboard Cite

The Arabidopsis SDS (SOLO DANCERS) and RCK (ROCK-N-ROLLERS) genes are important for male meiosis, but it is still unknown whether they represent conserved functions in plants. We have performed phylogenetic analyses of SDS and RCK and their respective homologs, and identified their putative orthologs in poplar and rice. Quantitative real-time RT-PCR analysis indicated that rice SDS and RCK are expressed preferentially in young flowers, and transgenic RNAi rice lines with reduced expression of these genes exhibited normal vegetative development, but showed significantly reduced fertility with partially sterile flowers and defective pollens. SDS deficiency also caused a decrease in pollen amounts. Further cytological examination of male meiocytes revealed that the SDS deficiency led to defects in homolog interaction and bivalent formation in meiotic prophase I, and RCK deficiency resulted in defective meiotic crossover formation. These results indicate that rice SDS and RCK genes have similar functions to their Arabidopsis orthologs. Because rice and Arabidopsis, respectively, are members of monocots and eudicots, two largest groups of flowering plants, our results suggest that the functions of SDS and RCK are likely conserved in flowering plants.

show abstract

The Saccharomyces cerevisiae MER3 gene, encoding a novel helicase-like protein, is required for crossover control in meiosis

Cited by 134 publications

References 47 publications

Sporulation in the Budding Yeast Saccharomyces cerevisiae

Sporulation in the Budding Yeast Saccharomyces cerevisiae

Exo1 Roles for Repair of DNA Double-Strand Breaks and Meiotic Crossing Over inSaccharomyces cerevisiae

Functional conservation of the meiotic genes SDS and RCK in male meiosis in the monocot rice

Contact Info

Product

Resources

About