The safener isoxadifen does not increase herbicide resistance evolution in recurrent selection with fenoxaprop

Rigon, Carlos Alberto Gonsiorkiewicz; Cutti, Luan; Angonese, Paula Sinigaglia; Sulzbach, Estéfani; Markus, Catarine; Gaines, Todd A.; Merotto, Aldo

doi:10.1016/j.plantsci.2021.111097

Cited by 4 publications

(11 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast to the ineffectiveness of safeners in dicots, several examples of metabolic resistance to herbicides are known in dicot weeds, , indicating substantial differences between safening in crops and metabolic resistance in weeds. This is further corroborated by a recent study on herbicide resistance evolution in Echinochloa crus-galli, demonstrating that the safener isoxadifen does not increase the evolution of fenoxaprop resistance in recurrent selection …”

Section: Introductionsupporting

confidence: 71%

“…This is further corroborated by a recent study on herbicide resistance evolution in Echinochloa crus-galli, demonstrating that the safener isoxadifen does not increase the evolution of fenoxaprop resistance in recurrent selection. 22 We recently reported on the safening activity and metabolism of the safener cyprosulfamide (CSA) against the acetolactate synthase (ALS) inhibitor thiencarbazone-methyl (TCM). 23 These two compounds were co-developed for the control of perennial and other problem weeds for pre-and post-emergence application in maize.…”

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Spatially Resolved Investigation of Herbicide–Safener Interaction in Maize (Zea mays L.) by MALDI-Imaging Mass Spectrometry

Kubicki

Giannakopoulos

Lamshöft

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

Monitoring agrochemical distribution within plant tissues delivers significant insights into the adsorption, distribution, metabolism, and elimination of agrochemicals. Detection and imaging of the safener cyprosulfamide (CSA) and the herbicide thiencarbazone-methyl (TCM) after micro-droplet application on the surface of maize leaves (Zea mays L.) have been achieved using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI). The agrochemicals were deposited onto the adaxial surface of maize leaves on growing plants, and their uptake, distribution, and metabolism were investigated at four timepoints (3 h, 24 h, 4 days, and 7 days) to assess the influence of CSA treatment on TCM metabolism. MALDI MSI visualized significant changes for the metabolism of TCM after 24 h. Although TCM metabolism was detected neither in the control without the safener nor in the approach with CSA on the second leaf, the co-application on the same leaf showed significant metabolism of the herbicide by detecting the metabolite N-demethylated TCM. These findings suggest that safener protection against herbicide injury is a rapid process in which CSA and TCM need to be present in the same tissues. This study showcases the use of MALDI MSI to visualize and analyze indirect interactions of two substances in planta.

show abstract

Section: Introductionsupporting

confidence: 71%

Section: ■ Introductionmentioning

confidence: 99%

Spatially Resolved Investigation of Herbicide–Safener Interaction in Maize (Zea mays L.) by MALDI-Imaging Mass Spectrometry

Kubicki

Giannakopoulos

Lamshöft

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…The greatest reduction in control in the G2 progeny occurred in the resistant biotype (POP2-IR). This biotype has a genetic background of enhanced herbicide metabolism . We speculate that this characteristic may enable faster resistance evolution under recurrent selection.…”

Section: Discussionmentioning

confidence: 95%

“…In preliminary studies, the MOST biotype was characterized as susceptible to acetolactate synthase (ALS) and acetyl-CoA carboxylase (ACCase) inhibitors, hereafter referred to as POP1-S. The CAMAQ01 biotype was characterized as resistant to imazethapyr with a resistance factor between 8.1 and 14 due to both target- and non-target-site mechanisms . This biotype has an amino acid change from Trp to Leu in position 574 of the ALS gene and when P450 inhibitors (malathion and PBO) were applied prior to imazethapyr, plants were better controlled, indicating that P450 enzymes are likely involved in imazethapyr metabolism .…”

Section: Methodsmentioning

confidence: 99%

“…Reference genes evaluated were 18S , 28S , EIF4B , and GAPDH commonly used in herbicide studies and identified as the most stable after herbicide treatments. ,,, Primers for housekeeping genes were already designed for the E. crus-galli biotype. , The candidate genes chosen were CYP71AK2 , CYP72A122 , CYP72A258 , CYP81A12 , CYP81A14 , CYP81A21 , CYP81A22 , and GST1 with primer sequences from previously conducted work on gene expression in E. crus-galli biotypes .…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Recurrent Selection of Echinochloa crus-galli with a Herbicide Mixture Reduces Progeny Sensitivity

Rigon

Cutti

Turra

et al. 2023

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Herbicide mixtures are used to increase the spectrum of weed control and to manage weeds with target-site resistance to some herbicides. However, the effect of mixtures on the evolution of herbicide resistance caused by enhanced metabolism is unknown. This study evaluated the effect of a fenoxaprop-p-ethyl and imazethapyr mixture on the evolution of herbicide resistance in Echinochloa crus-galli using recurrent selection at sublethal doses. The progeny from second generations selected with the mixture had lower control than parental plants or the unselected progeny. GR 50 increased 1.6-and 2.6-fold after two selection cycles with the mixture in susceptible (POP1-S) and imazethapyr-resistant (POP2-IR) biotypes, respectively. There was evidence that recurrent selection with this sublethal mixture had the potential to evolve cross-resistance to diclofop, cyhalofop, sethoxydim, and quinclorac. Mixture selection did not cause increased relative expression for a set of analyzed genes (CYP71AK2, CYP72A122, CYP72A258, CYP81A12, CYP81A14, CYP81A21, CYP81A22, and GST1). Fenoxaprop, rather than imazethapyr, is the main contributor to the decreased control in the progenies after recurrent selection with the mixture in low doses. This is the first study reporting the effect of a herbicide mixture at low doses on herbicide resistance evolution. The lack of control using the mixture may result in decreased herbicide sensitivity of the weed progenies. Using mixtures may select important detoxifying genes that have the potential to metabolize herbicides in patterns that cannot currently be predicted. The use of fully recommended herbicide rates in herbicide mixtures is recommended to reduce the risk of this type of resistance evolution.

show abstract

The safener isoxadifen‐ethyl confers fenoxaprop‐p‐ethyl resistance on a biotype of Echinochloa crus‐galli

Cutti

Rigon

Girelli

et al. 2022

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUND: Some herbicides are commercially formulated with safeners to increase crop selectivity. Fenoxaprop-p-ethyl is formulated with the safener isoxadifen-ethyl for Echinochloa crus-galli control in rice. Safeners act on crops by increasing herbicide metabolism, but this effect may also occur in weeds. The objective of this study was to investigate the effect of the safener isoxadifen-ethyl on the resistance to fenoxaprop-p-ethyl in a biotype of E. crus-galli.RESULTS: A screening of 52 biotypes identified lack of control in the biotype SANTPAT-R treated with the recommended dose of 69 g ha −1 of the commercial formulation of fenoxaprop-p-ethyl with the safener isoxadifen-ethyl. While this biotype survived doses greater than 2208 g ha −1 of the formulation fenoxaprop-p-ethyl + isoxadifen-ethyl, it was killed with 69 g ha −1 of fenoxaprop-p-ethyl without the safener. A glutathione-s-transferase (GST) enzymes inhibitor reduced the resistance factor in two dose-response curves. A minor effect of a CytP450 inhibitor was observed. The previous spraying of the safener isoxadifen-ethyl followed by fenoxaprop-p-ethyl induced survival in the resistant but not in the susceptible biotype. The GST1 and GSTF1 genes were up-regulated in the resistant biotype. ACCase gene mutations were not found, and no cross-resistance to other ACCase inhibitors was identified.CONCLUSION: The safener isoxadifen-ethyl present in the commercial herbicide formulation of fenoxaprop-p-ethyl is associated with resistance in the E. crus-galli SANTPAT-R biotype. This resistance is related with herbicide metabolization mediated by GST pathways. This is the first field-selected weed biotype with herbicide resistance due to safener presence in the sprayed formulation.

show abstract

The safener isoxadifen does not increase herbicide resistance evolution in recurrent selection with fenoxaprop

Cited by 4 publications

References 38 publications

Spatially Resolved Investigation of Herbicide–Safener Interaction in Maize (Zea mays L.) by MALDI-Imaging Mass Spectrometry

Spatially Resolved Investigation of Herbicide–Safener Interaction in Maize (Zea mays L.) by MALDI-Imaging Mass Spectrometry

Recurrent Selection of Echinochloa crus-galli with a Herbicide Mixture Reduces Progeny Sensitivity

The safener isoxadifen‐ethyl confers fenoxaprop‐p‐ethyl resistance on a biotype of Echinochloa crus‐galli

Contact Info

Product

Resources

About