The Safety and Efficacy of 1-Monoeicosapentaenoin Isolated from the Trebouxiophyceae Micractinium on Anti-Wrinkle: A Split-Face Randomized, Double-Blind Placebo-Controlled Clinical Study

Abstract

The skin aging process is governed by intrinsic and extrinsic factors causing skin wrinkles, sagging, and loosening. The 1-monoeicosapentaenoin (1-MEST) is a component isolated from Micractinium, a genus of microalgae (Chlorophyta, Trebouxiophyceae). However, the anti-wrinkle effects of 1-MEST are not yet known. This study aimed to evaluate the anti-wrinkle effects of 1-MEST in vitro and in clinical trials. The cytotoxicity of 1-MEST was investigated in vitro using the MTS assay in human epidermal keratinocytes (HEKs). Expression of matrix metalloproteinase (MMP)-1 and MMP-9 was determined by ELISA in HEKs irradiated with UVB after treatment with 1-MEST. A split-face randomized, double-blind, placebo-controlled study was conducted to evaluate the safety and efficacy of 1-MEST. The study evaluated wrinkle parameters and visual assessment, self-efficacy and usability questionnaires, and adverse events. The study showed that the 1-MEST was not cytotoxic in HEKs, suppressed MMP-1 secretion and MMP-9 protein expression in HEKs irradiated with UVB. The wrinkle parameters and mean visual assessment score were significantly decreased in the test group after 12 weeks and differed from the control group. There were no significant differences in efficacy and usability. Adverse effects were also not observed. The 1-MEST showed anti-wrinkle properties to slow down or prevent skin aging.

A study of anti‐wrinkle functions and improvement of cream with Phaseolus angularis

“…In comparison, in the placebo group, R1 remained unchanged, R3 decreased, and R2, R4 and R5 increased at 12 weeks compared to before use. A recent similar clinical study using a face cream containing 1‐Monoeicosapentaenoin isolated from the Trebouxiophyceae Micractinium for 12 weeks showed a similar decrease value with PASE in R1, R2 and R3, but R4 and R5 had a lower decrease rate [30]. Moreover, after 12 weeks of PASE cream use, there was a significant level of wrinkle improvement in all parameters compared to placebo use ( p < 0.05; Table 6).…”

Protective role of melatonin in ultraviolet radiation- induced oxidative stress in human skin photoaging

“…(23,34) Premature skin aging as a result of photoaging is characterized by deep coarse wrinkles, mottled irregular hyperpigmentation, thin, lax, and leathery skin texture mainly in exposed areas such as the skin of the face, neck, and hands, accompanied by lentigines, telangiectasias, and sallowness. (17,18,59,60) Solar UVR consists of electromagnetic wavelengths between 100 and 400 nm that reach the terrestrial surface and has a direct effect on human health and terrestrial and aquatic ecosystems, as well as material degradation. (61)(62)(63)(64) It is divided into three components, depending on its transmission capability in the atmosphere and its biological effects: UVA (315-400 nm) makes up most of the UVR received on the terrestrial surface; UVB (280-315 nm) which is partially absorbed into the atmosphere, and UVC (100-280 nm) which is completely absorbed by stratospheric ozone.…”