The Safety of Bone Allografts Used in Dentistry

Holtzclaw, Dan; Toscano, Nicholas; Eisenlohr, Lisa; Callan, Don

doi:10.14219/jada.archive.2008.0334

Cited by 60 publications

(42 citation statements)

References 7 publications

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“…Freeze-dried bone allografts undergo two size reduction phases (initial and secondary) in their manufacturing process (Holtzclaw et al 2008). Therefore, superior viability and differentiation in PDFDBA group with a wider range of particle sizes compared to the same type of allograft (from the same bank) but with smaller range of particle sizes in vitro may be related to the process of cutting and shaping of the particles and their effect on the quantity, quality, solubility and stability of osteoinductive water-soluble released materials.…”

Section: Discussionmentioning

confidence: 99%

In vitro effect of mineralized and demineralized bone allografts on proliferation and differentiation of MG-63 osteoblast-like cells

et al. 2015

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Due to the extensive use of bone allografts in bone reconstruction and periodontal therapy as suitable alternatives to autografts, they are now marketed under different commercial brands. Considering the controversial reports regarding the osteoinductive properties of bone allografts, this study sought to assess the effect of type (mineralized/demineralized), amount and particle size of several allografts on the proliferation and differentiation of MG-63 osteoblast-like cells. MG-63 cells (24-h culture) were exposed to 20 and 40 mg amounts of nine different commercially available freeze-dried bone allografts. After 24 and 72 h of incubation, the effect of water-soluble allograft released materials on cell viability and proliferation was assessed using methyl thiazol tetrazolium (MTT) assay after 24 and 72 h of exposure. Cell differentiation and mineralization was assessed by real-time quantitative reverse transcription PCR and alizarin red staining after 72 h of exposure. The amount and particle size of understudy allografts had significant effects on cell viability after 24 h of exposure (in contrast to 72 h). Higher rate of proliferation was seen in non-differentiated or slow-differentiated groups. The amount and particle size factors had no significant effect on the amount of calcified nodules or the expression of osteogenic marker genes in most groups. Faster and more distinct differentiation and mineralization was noted in mineralized compared to demineralized groups during the 3-day study period. Based on the results, the understudy mineralized (non-demineralized) bone allografts had greater effect on osteogenic differentiation of the MG-63 cells and showed more in vitro osteoinductive activity compared to partially demineralized and fully demineralized types.

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Section: Discussionmentioning

confidence: 99%

In vitro effect of mineralized and demineralized bone allografts on proliferation and differentiation of MG-63 osteoblast-like cells

et al. 2015

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“…O rigor nos protocolos de triagem de doadores e no processamento reduziu o risco de transmissão de doenças e a ocorrência de reações imunológicas (HOLTZCLAW et al, 2008;GROVER et al, 2011).…”

Section: Enxerto óSseo Alógenounclassified

“…A variabilidade do tempo de retirada do enxerto após a morte e as características do doador como a idade, o gênero, o local de retirada e as condições sistêmicas dificultam a padronização de sua qualidade, sendo uma desvantagem associada a esta técnica (KAO; SCOTT, 2007;MCALLISTER et al, 2007;HOLTZCLAW et al, 2008;ANNIBALI et al, 2011;GROVER et al, 2011).…”

Section: Enxerto óSseo Alógenounclassified

“…Em algumas áreas observaram-se áreas coradas levemente diferente do osso regenerado e lacuna osteocíticas vazias, indicando Ao trabalhar com enxertos de bancos de ossos é importante tomar o cuidado de conhecer e investigar cuidadosamente a origem dos produtos e o método de processamento utilizado. Devem-se priorizar os produtos de entidades registradas em agências de saúde e que seguem protocolos comprovadamente eficazes na prevenção de transmissão de doenças (HOLTZCLAW et al, 2008).…”

Section: Enxerto óSseo Alógenounclassified

See 1 more Smart Citation

Análise tomográfica da formação óssea em defeito segmentar na mandíbula de coelho preenchido com bloco de osso bovino liofilizado

Corrêa¹

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“…Assim, o osso é coletado de cadáver e submetido a vários tratamentos com o intuito de diminuir sua antigenicidade e preservar suas propriedades indutoras (Garbin Júnior, 2008). É tratado com medicamentos (como antibióticos), solventes e por criodesinfecção em câmara fria à -80ºC, podendo ainda ser submetido à liofilização, desmineralização ou irradiação (Salai et al, 2000;Nguyen et al, 2007;Holtzclaw et al, 2008). Esses enxertos são fornecidos por bancos de tecidos especialmente licenciados (Esposito et al, 2010;Esposito et al, 2014).…”

Section: Revisão De Literaturaunclassified

Avaliação microtomográfica e histomorfométrica comparativa de levantamento de seio maxilar em coelhos utilizando diferentes cerâmicas a base de fosfato de cálcio

Santos¹

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The Safety of Bone Allografts Used in Dentistry

Cited by 60 publications

References 7 publications

In vitro effect of mineralized and demineralized bone allografts on proliferation and differentiation of MG-63 osteoblast-like cells

In vitro effect of mineralized and demineralized bone allografts on proliferation and differentiation of MG-63 osteoblast-like cells

Análise tomográfica da formação óssea em defeito segmentar na mandíbula de coelho preenchido com bloco de osso bovino liofilizado

Avaliação microtomográfica e histomorfométrica comparativa de levantamento de seio maxilar em coelhos utilizando diferentes cerâmicas a base de fosfato de cálcio

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