The Salivary Microbiome and Oral Cancer Risk: A Pilot Study in Fanconi Anemia

Furquim, Camila Pinheiro; Soares, Geisla Mary Silva; Ribeiro, Lisandro; Azcarate‐Peril, M. Andrea; Butz, Natasha; Roach, Jeffrey; Moss, Kevin; Bonfim, Carmem; Torres‐Pereira, Cassius Carvalho; Teles, Flavia

doi:10.1177/0022034516678169

Cited by 49 publications

(35 citation statements)

References 39 publications

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“…In addition, the genus Streptococcus exhibited moderate classification potential. While normally harmless, these two genera ( Staphylococcus and Streptococcus ) are being growingly investigated in relationship to the development of cancer in different organ sites . These studies have shown conflicting descriptions of these colonizers in carcinogenesis.…”

Section: Discussionmentioning

confidence: 55%

“…While normally harmless, these two genera (Staphylococcus and Streptococcus) are being growingly investigated in relationship to the development of cancer in different organ sites. 18,[37][38][39][40][41][42][43] These studies have shown conflicting descriptions of these colonizers in carcinogenesis. Hosgood et al 18 reported enrichment of Streptococcus in lung cancer cases, consistent with our own findings, while Staphylococcus showed no significant differences between cases and controls.…”

Section: Discussionmentioning

confidence: 99%

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Difference of lower airway microbiome in bilateral protected specimen brush between lung cancer patients with unilateral lobar masses and control subjects

Liu

Tao

Zhang

et al. 2017

Intl Journal of Cancer

170

156

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The functional role of respiratory microbiota has attracted an accumulating attention recently. However, the role of respiratory microbiome in lung carcinogenesis is mostly unknown. Our study aimed to characterize and compare bilateral lower airway microbiome of lung cancer patients with unilateral lobar masses and control subjects. Protected bronchial specimen brushing samples were collected from 24 lung cancer patients with unilateral lobar masses (paired samples from cancerous site and the contralateral noncancerous site) and 18 healthy controls undergoing bronchoscopies and further analyzed by 16S rRNA amplicon sequencing. As results, significant decreases in microbial diversity were observed in patients with lung cancer in comparison to the controls, alpha diversity steadily declined from healthy site to noncancerous to cancerous site. Genus Streptococcus was significantly more abundant in cancer cases than the controls, while Staphylococcus was more abundant in the controls. The area under the curve of genus Streptococcus used to predict lung cancer was 0.693 (sensitivity = 87.5%, specificity = 55.6%). The abundance of genus Streptococcus and Neisseria displayed an increasing trend whereas Staphylococcus and Dialister gradually declined from healthy to noncancerous to cancerous site. Collectively, lung cancer-associated microbiota profile is distinct from that found in healthy controls, and the altered cancer-associated microbiota is not restricted to tumor tissue. The genus Streptococcus was abundant in lung cancer patients and exhibited moderate classification potential. The gradual microbiota profile shift from healthy site to noncancerous to paired cancerous site suggested a change of the microenvironment associated with the development of lung cancer.

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Difference of lower airway microbiome in bilateral protected specimen brush between lung cancer patients with unilateral lobar masses and control subjects

Liu

Tao

Zhang

et al. 2017

Intl Journal of Cancer

170

156

View full text Add to dashboard Cite

show abstract

“…Noteworthy, recent work has highlighted the critical role of metabolic products from specific gut microbiota such as lactobacilli in priming IL-22 dependent mucosal immune responses by innate lymphoid cells via the aryl hydrocarbon receptor, which is fundamental for protection against uncontrolled local Candida expansion [41]. Further, a shift in salivary microbiota has been linked to the risk of oral cancer in selected groups of patients [42]. We found significantly increased abundance of streptococci in APS-1 saliva compared to healthy controls and several streptococci were among the most abundant species in APS-1 saliva.…”

Section: Discussionmentioning

confidence: 99%

Oral microbiota in autoimmune polyendocrine syndrome type 1

Bruserud

Siddiqui

Marthinussen

et al. 2018

Journal of Oral Microbiology

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Background: Autoimmune polyendocrine syndrome type-1 (APS-1) is a rare, childhood onset disease caused by mutations in the Autoimmune Regulator gene. The phenotypic expression is highly variable and includes disease manifestations in the oral cavity, including mucocutaneous candidiasis. Increasing evidence suggests a potential role of the skin, oral and gut microbiotas in the pathogenesis of autoimmunity. To date, no information exists regarding the oral microbiota in APS-1. Objective: To assess the bacterial microbiota of whole saliva in APS-1 patients by using high throughput sequencing. Design: Whole unstimulated saliva was collected from 10 APS-1 patients and 17 healthy controls and examined by high throughput sequencing of the hypervariable region V1-V2 of 16S rRNA using the 454 GS Junior system. Metastats (http://cbcb.umd.edu/software/metastats) was used to analyse the pyrosequencing reads. Results: A reduction in the total number of bacterial genera and species was detected in APS-1 compared to healthy controls. The proportion of the major phyla Firmicutes was higher (60% vs 41%, p = 0.002) and Bacteroidetes lower (15% vs 28%, p = 0.007) in APS-1 compared to healthy controls. On the genus level, Streptococcus and Gemella were prevalent in APS-1. Conclusion: Our findings indicate a significantly altered oral microbiota in APS-1.

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“…The risk of HNSCC increases with age, but compared with the general population, patients with FA develop these tumors at much earlier ages, with a median age at diagnosis in the early 30s. Many hypotheses, including human papillomavirus infection, exposure to aldehydes, and chronic infections, have been investigated. However, to the best of our knowledge, the rationale for the 500‐fold to 700‐fold elevated risk of HNSCC in the FA population still has yet to be identified.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic accuracy of brush biopsy–based cytology for the early detection of oral cancer and precursors in Fanconi anemia

Velleuer

Dietrich²,

Pomjanski

et al. 2020

Cancer Cytopathology

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BACKGROUND: Individuals with Fanconi anemia (FA) have a 500-fold to 700-fold elevated risk, much earlier onset, and limited therapeutic options for oral squamous cell carcinoma (SCC) compared with the general population. The early detection of SCC, or preferably its precursors, is mandatory to retain curative therapeutic options. Due to frequent synchronic and metachronic oral lesions, tissue biopsies, as usually recommended by guidelines, often are not feasible. In the current study, an alternative strategy for early detection using oral brush biopsy-based cytology was validated regarding its diagnostic accuracy. METHODS: Over a 12-year period, the oral cavities of a large cohort of 713 individuals with FA were inspected systematically and brush biopsy-based cytology of 1233 visible oral lesions was performed. In cases of inconclusive cytology, analysis of DNA ploidy was performed whenever possible. The results were correlated to a long-term clinicopathological follow-up reference standard. RESULTS: A total of 737 lesions were suitable for statistical analysis, including 86 lesions with at least high-grade oral epithelial dysplasia in 30 patients. For cytology, the sensitivity and specificity were 97.7% and 84.5%, respectively. Additional analysis of DNA ploidy increased the sensitivity and specificity to 100% and 92.2%, respectively. CONCLUSIONS: Careful inspection of the oral cavity of individuals with FA followed by brush biopsy-based cytology appears to identify visible oral, potentially malignant and malignant lesions that warrant treatment. Approximately 63% of SCC and precursor lesions are detected at a noninvasive or early stage. Negative cytology or a lack of DNA aneuploidy can exclude high-grade oral epithelial dysplasia or SCC with high accuracy and thus reduce the need for invasive diagnostic biopsies. Cancer Cytopathol 2020;128:403-413.Attri butio n-NonCo mmerc ial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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The Salivary Microbiome and Oral Cancer Risk: A Pilot Study in Fanconi Anemia

Cited by 49 publications

References 39 publications

Difference of lower airway microbiome in bilateral protected specimen brush between lung cancer patients with unilateral lobar masses and control subjects

Difference of lower airway microbiome in bilateral protected specimen brush between lung cancer patients with unilateral lobar masses and control subjects

Oral microbiota in autoimmune polyendocrine syndrome type 1

Diagnostic accuracy of brush biopsy–based cytology for the early detection of oral cancer and precursors in Fanconi anemia

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