The Salmonella typhimurium uracil-sensitive mutation use is in argU and encodes a minor arginine tRNA

Lu, Chung‐Dar; Abdelal, Ahmed T.

doi:10.1128/jb.175.12.3897-3899.1993

Cited by 8 publications

(5 citation statements)

References 22 publications

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“…Indeed, it is possible that the proximity of the ribosome binding site to the rho-independent terminator of the carA transcript in P. aeruginosa obviates the requirement for a transcriptional pause site. Moreover, if pyrimidine control of carA in P. aeruginosa actually occurs through an attenuationtype mechanism, this would be in marked contrast to that observed for carAB in enteric bacteria, where (13,28,31). Pyrimidine-insensitive expression of a carA::lacZ fusion (pKA26 ; Table 3), which is still mediated by arginine availability in the cell, indicates that regulation of transcriptional initiation for this system in P. aeruginosa is exclusive to arginine control.…”

Section: Discussionmentioning

confidence: 94%

Structure and regulation of the carAB operon in Pseudomonas aeruginosa and Pseudomonas stutzeri: no untranslated region exists

Kwon

Walthall

et al. 1994

J Bacteriol

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The carAB operons from Pseudomonas aeruginosa PAO1 and Pseudomonas stutzeri JM300 were characterized by Southern and DNA sequence analyses. The results show that the previously reported sequence for carA (S. C. Wong and A. T. Abdelal, J. Bacteriol. 172:630-642, 1990) is derived from P. stutzeri and not P. aeruginosa, as originally reported. Therefore, the amino-terminal sequence of the purified carA product is identical to that derived from the nucleotide sequence in both organisms, P. stutzeri having four additional amino acids. The results also show that while carA and carB are contiguous in P. stutzeri, as is the case in other bacteria, they are surprisingly separated by an open reading frame (ORF) of 216 amino acids in P. aeruginosa. S1 nuclease mapping experiments with RNA extracted under a variety of growth conditions, as well as experiments using different lacZ fusions, indicate that the carA-ORF-carB operon of P. aeruginosa is transcribed from a single promoter. Moreover, these experiments demonstrate that expression of this single transcript is controlled by both arginine and pyrimidines and that variation in arginine levels specifically modulates transcriptional initiation, while pyrimidine regulation is exerted subsequent to transcriptional initiation. Modification of a rho-independent terminator-like structure, which is present upstream of carA in P. aeruginosa, removed all transcriptional sensitivity of a carA::lacZ fusion to pyrimidines. This result, when coupled with the finding that translation of an 18-amino-acid leader polypeptide (associated with this putative rho-independent terminator), is inversely proportional to pyrimidine concentration in the cell, strongly suggests that regulation of carA by pyrimidines is mediated through an attenuation-type mechanism in P. aeruginosa.

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Section: Discussionmentioning

confidence: 94%

Structure and regulation of the carAB operon in Pseudomonas aeruginosa and Pseudomonas stutzeri: no untranslated region exists

Kwon

Walthall

et al. 1994

J Bacteriol

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“…Plasmid insertion and rescue. The first stage of plasmid insertion and rescue involves cloning an appropriate fragment of pneumococcal DNA into pVA891 (which encodes chloramphenicol and erythromycin resistance but cannot replicate in S. pneumoniae [27]) and using this construct to transform Rxl-19F. Recombination between the pneumococcal DNA insert and the homologous region of the pneumococcal chromosome results in integration of the plasmid sequences.…”

Section: Materlals and Methodsmentioning

confidence: 99%

“…10. Alignment of the amino acid sequences of Cps19fF, B. subtilis TagA (BsTagA) (30), E. coli K-12 RffM (EcRffM) (8), and Salmonella typhimurium LT2 RffM (LT2RffM) (27), as determined using the default settings of the program CLUSTAL (20). Identical residues are boxed; similar residues are shown in boldface.…”

Section: Lt2rfbpmentioning

confidence: 99%

“…The cpsl9fF gene product has homology (31 to 33% identity) with three other proteins, namely, the B. subtilis tagA gene product which is needed for cell wall teichoic acid biosynthesis (30) and the E. coli K-12 and Salmonella enterica serovar typhimurium strain LT2 raM gene products, which are putative UDP-N-acetyl-D-mannosaminuronic acid transferases, involved in the synthesis of enterobacterial common antigen (8,27). An alignment of their amino acid sequences (Fig.…”

Section: Lt2rfbpmentioning

confidence: 99%

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Nucleotide sequence analysis of genes essential for capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 19F

et al. 1994

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“…1). In contrast, while the absence of pyrimidine control under high-copy conditions (Kilstrup et aL, 1988;Bouvier et aL, 1984) indicates that pyrimidine control is mediated by a titratable regulatory element, such an element has yet to be identified (Lu and Abdelal, 1993).…”

confidence: 99%

Participation of the purine repressor in control of the carbamoylphosphate synthetase operon in Salmonella typhimurium

Katzif

Abdelal³

1995

Molecular Microbiology

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Previous work has shown that the carAB operon of Salmonella typhimurium is transcribed from tandem promoters, P1 and P2, that are negatively controlled by pyrimidines and arginine, respectively. The results reported here show that purines also negatively control expression of carAB and that this effect is absent in a purR::Tn10 derivative. Primer-extension experiments established that the purine effect is exerted at P1, thus redefining this promoter as sensitive to both purines and pyrimidines. The results of gel-retardation experiments as well as DNase I and premethylation footprintings indicate that the purine repressor interacts with a PUR box 85 bp upstream of P1. Modification of this PUR box by site-directed mutagenesis abolishes the repression by purines in a carA::lacZ fusion, confirming that this box functions in vivo in purine control of carAB expression.

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The Salmonella typhimurium uracil-sensitive mutation use is in argU and encodes a minor arginine tRNA

Cited by 8 publications

References 22 publications

Structure and regulation of the carAB operon in Pseudomonas aeruginosa and Pseudomonas stutzeri: no untranslated region exists

Structure and regulation of the carAB operon in Pseudomonas aeruginosa and Pseudomonas stutzeri: no untranslated region exists

Nucleotide sequence analysis of genes essential for capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 19F

Participation of the purine repressor in control of the carbamoylphosphate synthetase operon in Salmonella typhimurium

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