The same but different: signaling pathways in control of endothelial cell migration

Hasan, Sana S.; Siekmann, Arndt F.

doi:10.1016/j.ceb.2015.07.009

Cited by 25 publications

(18 citation statements)

References 60 publications

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“…In a landmark study, Xu et al ( 2014 ) showed that regenerating vessels in the regenerating tail fin originate from vein-derived cells that acquire angiogenic potential. These cells migrate singly or collectively and organize into vessel in response to chemokine signaling (reviewed by Hasan and Siekmann, 2015 ). However, the applicability of this model to the study of vascular regeneration could be much more widely exploited.…”

Section: Zebrafish As a Model For Angiogenesis Researchmentioning

confidence: 99%

Zebrafish as an Emerging Model Organism to Study Angiogenesis in Development and Regeneration

Chávez¹,

et al. 2016

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Angiogenesis is the process through which new blood vessels are formed from preexisting ones and plays a critical role in several conditions including embryonic development, tissue repair and disease. Moreover, enhanced therapeutic angiogenesis is a major goal in the field of regenerative medicine and efficient vascularization of artificial tissues and organs is one of the main hindrances in the implementation of tissue engineering approaches, while, on the other hand, inhibition of angiogenesis is a key therapeutic target to inhibit for instance tumor growth. During the last decades, the understanding of cellular and molecular mechanisms involved in this process has been matter of intense research. In this regard, several in vitro and in vivo models have been established to visualize and study migration of endothelial progenitor cells, formation of endothelial tubules and the generation of new vascular networks, while assessing the conditions and treatments that either promote or inhibit such processes. In this review, we address and compare the most commonly used experimental models to study angiogenesis in vitro and in vivo. In particular, we focus on the implementation of the zebrafish (Danio rerio) as a model to study angiogenesis and discuss the advantages and not yet explored possibilities of its use as model organism.

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Section: Zebrafish As a Model For Angiogenesis Researchmentioning

confidence: 99%

Zebrafish as an Emerging Model Organism to Study Angiogenesis in Development and Regeneration

Chávez¹,

et al. 2016

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“…The emergence of these sprouts is guided by several factors (reviewed in Hasan and Siekmann, 2015), including attractive signals (e.g. Vegf-A) and repulsive signals (e.g.…”

Section: Tip Cell Selection and Functionmentioning

confidence: 99%

Cell behaviors and dynamics during angiogenesis

et al. 2016

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Vascular networks are formed and maintained through a multitude of angiogenic processes, such as sprouting, anastomosis and pruning. Only recently has it become possible to study the behavior of the endothelial cells that contribute to these networks at a single-cell level in vivo. This Review summarizes what is known about endothelial cell behavior during developmental angiogenesis, focusing on the morphogenetic changes that these cells undergo.

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“…Thirdly, VEGF-induced Rac1 activation in ECs is mainly mediated by Vav2 phosphorylation 28 , while so far there is no evidence showing that DOCK180 is directly stimulated by VEGF in mammalian cells. Moreover, DOCK180 deficiency results in vascular defects but has no impact on VEGF-induced EC migration (reviewed in) 8 , further supporting that DOCK180 is not directly linked to VEGFR signalling. Taken these together, we propose that stimulation of the DOCK180 pathway by PTP1B inhibitor, which does not require concomitant VEGFR2 activation as a prerequisite, may represent an important alternative mechanism of PTP1B inhibitor-stimulated EC motility (see Supplementary Fig.…”

Section: Discussionmentioning

confidence: 94%

“…Increased EC migration is also favourable for re-endothelialization of the denuded luminal surface of injured blood vessels, which is critical to prevent the development of intimal hyperplasia and stenosis following mechanical vessel injuries 4 5 . EC migration is coordinated by complex signalling mechanisms, of which those mediated by vascular endothelial growth factor receptors (VEGFRs) and the Rho family small GTPases have critical roles 6 7 8 . VEGF is one of the most important chemotactic factors that guide the directional movement of endothelial cells 6 .…”

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confidence: 99%

PTP1B inhibitor promotes endothelial cell motility by activating the DOCK180/Rac1 pathway

Wang

Yan

et al. 2016

Sci Rep

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Promoting endothelial cell (EC) migration is important not only for therapeutic angiogenesis, but also for accelerating re-endothelialization after vessel injury. Several recent studies have shown that inhibition of protein tyrosine phosphatase 1B (PTP1B) may promote EC migration and angiogenesis by enhancing the vascular endothelial growth factor receptor-2 (VEGFR2) signalling. In the present study, we demonstrated that PTP1B inhibitor could promote EC adhesion, spreading and migration, which were abolished by the inhibitor of Rac1 but not RhoA GTPase. PTP1B inhibitor significantly increased phosphorylation of p130Cas, and the interactions among p130Cas, Crk and DOCK180; whereas the phosphorylation levels of focal adhesion kinase, Src, paxillin, or Vav2 were unchanged. Gene silencing of DOCK180, but not Vav2, abrogated the effects of PTP1B inhibitor on EC motility. The effects of PTP1B inhibitor on EC motility and p130Cas/DOCK180 activation persisted in the presence of the VEGFR2 antagonist. In conclusion, we suggest that stimulation of the DOCK180 pathway represents an alternative mechanism of PTP1B inhibitor-stimulated EC motility, which does not require concomitant VEGFR2 activation as a prerequisite. Therefore, PTP1B inhibitor may be a useful therapeutic strategy for promoting EC migration in cardiovascular patients in which the VEGF/VEGFR functions are compromised.

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The same but different: signaling pathways in control of endothelial cell migration

Cited by 25 publications

References 60 publications

Zebrafish as an Emerging Model Organism to Study Angiogenesis in Development and Regeneration

Zebrafish as an Emerging Model Organism to Study Angiogenesis in Development and Regeneration

Cell behaviors and dynamics during angiogenesis

PTP1B inhibitor promotes endothelial cell motility by activating the DOCK180/Rac1 pathway

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