The sanitizing action of essential oil-based solutions against Salmonella enterica serotype Enteritidis S64 biofilm formation on AISI 304 stainless steel

Abstract

a b s t r a c tBacterial adhesion and biofilm formation by Salmonella enterica serovar Enteritidis is a serious concern in the food processing industry; organism persistence in biofilms represents a continual source of contamination. Due to unsuccessful disinfection processes and emerging resistance, conventional control methods are rapidly becoming ineffective, necessitating the development of new control strategies. The following study evaluated the anti-biofilm effect of disinfectant solutions formulated with essential oils (EOs) of peppermint (Mentha piperita) and lemongrass (Cymbopogon citratus) against biofilm formation by S. enterica serotype Enteritidis S64 on stainless steel surface AISI 304 (#4) after 10, 20 and 40 min of contact. A minimum inhibitory concentration (MIC) of 7.8 mL/mL was found for both EOs and disinfectant solutions were formulated based on these MIC values. Ten minutes of sanitizing solution contact significantly reduced (p 0.05) adhered bacterial populations for both EOs tested. After 20 and 40 min of treatment, cell counts were not detected. Thus, M. piperita and C. citratus EOs can be considered convenient, quality alternatives to the application of conventional sanitizing agents in the food industry; further, use of these EOs addresses the increasing consumer demand for natural products.

Effect of cinnamon essential oil and cinnamaldehyde on Salmonella Saintpaul biofilm on a stainless steel surface

“…Recently, essential oils have been used to control bacterial biofilms (Budzyñska et al, 2011;Oliveira et al, 2010;Valeriano et al, 2012), but few studies have evaluated the action of cinnamon EO and cinnamaldehyde. The effects of these compounds against Listeria monocytogenes, Escherichia coli, Pseudomonas spp., and Staphylococcus spp.…”

Anti-biofilm Activities and Chemical Composition of Essential Oil from Burdock Leaf

“…Plant essential oils have been used for thousands of years all over the world (Ozkan et al, 2010;Valeriano et al, 2012). A great many of reports have suggested that some of the essential oils are useful in food preservation (Bendimerad et al, 2005;Mimica-Dukic et al, 2004) and also exhibit pharmacological properties such as antibacterial (Lo Cantore et al, 2009), spasmolytic (Olivier et al, 2009), antioxidant (Güllüce et al, 2003) and anticancer activities (Ashour, 2008).…”

Microbial Biofilms: The Challenge of Food Industry

“…Winkelstroter et al [11] and Winkelstroter et al [12] evaluated the use of antimicrobial molecules known as bacteriocins and that researchers found a potential use of them to influence Listeria monocytogenes biofilm formation. Valeriano et al [13] evaluated the anti-biofilm effect of with essential oils (EOs) of peppermint (Mentha piperita) and lemongrass (Cymbopogon citratus) against biofilm formation by S. enterica serotype Enteritidis S64.…”