The scallop IGF2 mRNA-binding protein gene <i>PyIMP</i> and association of a synonymous mutation with growth traits

Abstract

Insulin-like growth factor 2 mRNA-binding proteins (IMPs) function in localization, stability and translational control of their target RNAs. In this study, we identified an IMP gene (PyIMP) from Yesso scallop, Patinopecten yessoensis. The complete DNA sequence of PyIMP was 22,875 bp, consisting of seventeen exons and sixteen introns. The full-length cDNA sequence was 3,293 bp, with an open reading frame of 1,776 bp, encoding 592 amino acids. PyIMP exhibited characters typical of IMPs, namely two RNA recognition motifs and four hnRNP K homology domains. Real-time quantitative reverse transcription PCR analysis indicated that PyIMP was universally expressed, with higher expression levels in the gonad of adult scallops, and in gastrulae and trochophore larvae at developmental stages. A synonymous mutation SNP, c.852A>G, which showed significant associations with growth traits of Yesso scallop, was identified in this gene. Scallops with the AA genotype at this locus had significantly higher trait values than those with the GG genotype for shell length, shell height, body weight, soft tissue weight and striated muscle weight (P < 0.05). Meanwhile, the expression of PyIMP in AA type scallops was significantly higher than that in the GG type, implying a positive effect of PyIMP on scallop growth. PyIMP represents the first mRNA-binding protein gene characterized in mollusks, and SNP c.852A>G will be useful for a better understanding of the role of mRNA-binding proteins in bivalves and for scallop breeding.

Vital Role of Glutamate Dehydrogenase Gene in Ammonia Detoxification and the Association Between its SNPs and Ammonia Tolerance in Sinonovacula constricta

“…Increasing evidence has shown that some SNPs are associated with growth, disease resistance, and other economic traits of clams ( He et al, 2012 ; Siva et al, 2012 , 2016 ; Nie et al, 2015 ; Niu et al, 2015 ; Yao et al, 2020 ; Zhao et al, 2020 ). For instance, a synonymous mutation SNP c.852A > G of insulin-like growth factor 2 mRNA-binding protein gene in Patinopecten yessoensis showed a significant association with the growth traits ( Ning et al, 2018 ), while two SNPs HbIIA-E2-146 and HbIIB-E2-23 of the hemoglobin gene ( Hb ) in Tegillarca granosa were associated with the Vibrio parahaemolyticus resistance ( Bao et al, 2013 ). The +2248T/C and +2365T/C of interferon regulatory factor 2 ( IRF -2) gene in freshwater mussel Hyriopsis cumingii were significantly related to Aeromonas hydrophila resistance ( Wang et al, 2013 ).…”

Mutations in Growth-Related Genes Induced by EMS Treatment in Scallops

“…The bay scallop Argopecten irradians is a commercially important bivalve widely cultured in northern China ( Ning et al, 2018 ). The cultured population of the bay scallops originated from only a small number of brood stocks survived from the last attempt of 3 consecutive introductions in early 1980s ( Zhang et al, 1986 ).…”

Genome‐wide association study analysis to resolve the key regulatory mechanism of biomineralization in Pinctada fucata martensii

“…For example, insulin/ insulin‐like growth factor (IGF) homologues, molluscan insulin‐like peptides (MIPs), and related regulation systems were identified from mollusc species. The identified endocrine system was considered to significantly influence body growth and regulate cell proliferation, cell activity, and glycogen metabolism (Choi et al, 2018; Ning et al, 2018; Zhang & He, 2020). The insulin/IGF pathway exerts its GTP‐dependent stimulation on the adenylyl cyclase activity by activating the mitogen‐activated protein kinase (MAPK) and phosphatidylinositol 3‐kinase (PI3K) pathways.…”