The Schistosoma Japonicum Egg Granuloma

Ks, Warren; Di, Grove; Rp, Pelley

doi:10.4269/ajtmh.1978.27.271

Cited by 58 publications

(35 citation statements)

References 3 publications

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“…This differs from the effect of route of injection of lyophilized eggs in the high responder strain, C57BL/6. In confirmation of previous results (Warren et al, 1975), a low level of sensitization has been used to demonstrate a difference between I.P. and S.C. injections in sensitization for lung AGH to eggs (Mitchell et al, 1982; Table 2).…”

Section: Variations In Lung Agh In Micementioning

confidence: 58%

“…Aspects explored include mouse strain variations in AGH expression (Mitchell et al, 1981a(Mitchell et al, , 1982, and, in mice of high or low responder status, the influence of route of injection of eggs or antigens with or without adjuvants for sensitization (Warren et al, 1975). A search for modulating effects of hyperimmune anti-egg antisera has also been performed (Olds et al, 1982).…”

Section: Introductionmentioning

confidence: 99%

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ATTEMPTS TO MODIFY LUNG GRANULOMATOUS RESPONSES TO SCHISTOSOMA JAPONICUM EGGS IN LOW AND HIGH RESPONDER MOUSE STRAINS

Mitchell¹,

Cruise²,

Garcia³

et al. 1983

Aust J Exp Biol Med

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Summary.A radioisotopic assay for acute granulomatous hypersensitivity (AGH) to lyophilized eggs of Schistosoma japonicum has been used to further examine responses to egg antigens in various inbred strains of mice. The ranking of responsiveness in mice from high (C57BL/6), intermediate (BALB/c) to low (CBA/H) was not influenced by high or low egg-sensitization regimes. However, the low responsiveness of responder mice sensitized with eggs by the intraperitoneal compared with the subcutaneous route of injection appears to be an egg dose-related phenomenon. The high AGH responsiveness of C57BL/6 mice can be increased further by sensitization with eggs in the presence of purified pertussigen from Bordetella pertussis but CBA/H mice treated identically remain low responders.The monoclonal anti-egg antibody, P.41, which produces a prominent blebtype circumoval precipitate with eggs, has been shown to be directed against major 'immunopathologic antigen(s)' of S. japonicum eggs. Thus, C57BL/6 mice were sensitized for AGH by injection of soluble extracted egg antigen (SEA) bound to an immunoabsorbent of P.41 antibody on Sepharose. No success has been achieved in modulating AGH in C57BL/6 mice by injection of hyperimmune antisera raised against lyophilized eggs in either high or low responder mouse strains. This failure is in line with previous results using antisera as well as monoclonal anti-egg antibodies. The consistent failure to demonstrate a modulating effect of antibodies in this compared with other laboratories may be related to the use of lyophilized rather than viable eggs. The data suggest that activities of antisera in granuloma modulation in murine schistosomiasis japonica result from egg destruction or inhibition of production of immunopathologic antigens by eggs rather than through effects on immunopathologic immune responses.Abbreviations used in this paper: AGH, acute granulomatous hypersensitivity; DTH, delayed-type hypersensitivity; EA, extracted egg antigen; FCA, Freund's complete adjuvant; FIA, Freund's incomplete adjuvant; FUdR, fluorodeoxyuridine; I.P., intraperitoneal(ly); IUdR, iododeoxyuridine; I.V. intra venous (ly); K, kidney; L, lung; NMS, normal mouse serum; NS, not significant; PBS, mouse tonicity phosphate buffered saline pH 7-3; P.41 and P.26, designations of two IgM anti-schistosome monoclonal antibodies; RIA, radioimmunoassay; S.C, subcutaneous(ly); SEA, soluble egg antigen; SPF, specific pathogen-free; T cell, thymusderived (influenced) cell.

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Section: Variations In Lung Agh In Micementioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

ATTEMPTS TO MODIFY LUNG GRANULOMATOUS RESPONSES TO SCHISTOSOMA JAPONICUM EGGS IN LOW AND HIGH RESPONDER MOUSE STRAINS

Mitchell¹,

Cruise²,

Garcia³

et al. 1983

Aust J Exp Biol Med

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“…Approximately 200 million humans and a significant number of livestock are infected with schistosomes, and approximately 20 million patients suffer from severe complications 18 . Severe complications of schistosomiasis have been reported after only a few weeks of exposure [13][14][15] and these patients are at a high risk of developing chronic manifestations with irreversible fibrosis 17 . Despite the public health importance of schistosomiasis and the risk that the disease might spread and intensify further in the absence of wide-ranging measures to improve it, schistosomiasis has been neglected for decades as a result of many consistent factors 16 .…”

Section: Introductionmentioning

confidence: 99%

The Schistosomula Tegument Antigen as a Potential Candidate for the Early Serological Diagnosis of Schistosomiasis Mansoni

Grenfell

Martins

Silva-Moraes

et al. 2013

Rev. Inst. Med. trop. S. Paulo

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SUMMARYIf Schistosoma mansoni infection could be detected in its early stages, especially before the egg deposition in the host tissues, the development of severe pathologic lesions could be efficiently prevented. We therefore developed an indirect enzyme-linked immunosorbent assay based on the detection of specific IgG against schistosomula antigens (ELISA-SmTeg). The assay was applied in sera samples from non-infected and infected mice collected seven and 15 days post-infection. The results were compared to the number of adult worms obtained by perfusion of the murine hepatic system 50 days post-infection. The sensitivity and specificity of the ELISA-SmTeg were 100% (p = 0.0032 and 0.0048 respectively for seven and 15 days of infection) with a cutoff value of 0.15 (p = 0.0002). Our findings show a novel low-cost serological assay using antigens which are easy to obtain, which was able to detect all the infected mice as early as seven days post-infection.

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“…Eosinophils are the major constituent of the eggassociated granulomatous lesions in schistosomiasis japonica [Warren and Domingo, 1970;Warren et al, 1975], As to the mechanisms involved in the accumu lation of eosinophils around schistosome eggs, delayed-type hypersensitivity reaction seems to be im portant in schistosomiasis mansoni [Boros and War ren, 1970]. Thus, lymphokines such as eosinophil stim ulating promotor (ESP) [Colley, 1973;Lewis et al, 1977] was detected in the culture supernatant of spleen cells obtained from Schistosoma mansoni-in fected mice.…”

Section: Introductionmentioning

confidence: 99%

Eosinophil Chemotactic Lymphokine Produced by Spleen Cells of <i>Schistosoma japonicu</i><i>m</i>-lnfected Mice

Owhashi¹,

Nawa²

1987

Int Arch Allergy Immunol

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Eosinophil chemotactic factor (ECF) was detected in the cell-free supernatant of the culture of spleen cells obtained from Schistosoma japonicum-infected mice. ECF production by spleen cells was dependent on both the amount of soluble egg antigen (SEA) added and the number of cells in the culture. When sufficient amount of SEA was added to the culture, ECF production was detectable by 6 h after incubation, reached a plateau by 24 h and then rapidly decreased. ECF was produced by nonadherent, Thy 1.2-positive cells, indicating that it is a lymphokine. After Sephadex G-75 gel chromatography, estimated molecular weight of this ECF was 15,000–20,000 daltons. When eosinophils were preincubated with this ECF lymphokine, their chemotactic reactivity to S.japonicum egg-derived ECF was significantly enhanced, although this pretreatment caused neither the deactivation of chemotactic reactivity to homologous ECF nor the enhancement of the random migration of eosinophils. Thus, this ECF lymphokine seems to be important not only as a chemoattractant but also as an activator of eosinophilotaxis around deposited eggs in schistosomiasis japonica.

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The Schistosoma Japonicum Egg Granuloma

Cited by 58 publications

References 3 publications

ATTEMPTS TO MODIFY LUNG GRANULOMATOUS RESPONSES TO SCHISTOSOMA JAPONICUM EGGS IN LOW AND HIGH RESPONDER MOUSE STRAINS

ATTEMPTS TO MODIFY LUNG GRANULOMATOUS RESPONSES TO SCHISTOSOMA JAPONICUM EGGS IN LOW AND HIGH RESPONDER MOUSE STRAINS

The Schistosomula Tegument Antigen as a Potential Candidate for the Early Serological Diagnosis of Schistosomiasis Mansoni

Eosinophil Chemotactic Lymphokine Produced by Spleen Cells of <i>Schistosoma japonicu</i><i>m</i>-lnfected Mice

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